Comparison of Pea Enation Mosaic Virus (PEMV) Isolates: Sequence of Coat Protein and Aphid Transmission Protein Genes of a European Isolate

Salgueiro, S.; Hull, Roger

doi:10.1111/j.1439-0434.1999.tb03839.x

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…aphids for PEMV) or wounding to invade the cells of their hosts (Salgueiro & Hull 1999). There is growing evidence that even tiny wounding caused by aphids has a deleterious effect on plant metabolism (Reymond et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus

Kyseláková¹,

Sedlářová²,

Kubala³

et al. 2013

Plant Prot. Sci.

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Kyseláková H., Sedlářová M., Kubala M., Nožková V., Piterková J., Luhová L., Novák O., Ilík P. (2013): Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus. Plant Protect. Sci., 49: 105-119.The physiological responses of pea plants during 40 days of compatible interaction with Pea enation mosaic virus (PEMV) were evaluated. PEMV induces systemic changes in the concentration of phytohormones. At 5 days post inoculation (dpi), a simultaneous increase in abscisic acid (ABA) level and a decrease in salicylic acid (SA) level was observed, which is known to be involved in the suppression of hypersensitive reaction. In our pathosystem it preceded the virus presence in the systemic leaves. PEMV induces the accumulation of ABA, SA and Hsp70 and enhances peroxidase (POX) activity at 15 dpi, when it is already transmitted throughout the plant. The formation of enations was preceded by a local accumulation of nitric oxide, which was followed by the appearance of reactive oxygen species, mostly in the vicinity of leaf veins. Such heterogeneity suggests involvement of these molecules in the control of hyperplasia and tissue differentiation.

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Section: Discussionmentioning

confidence: 99%

Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus

Kyseláková¹,

Sedlářová²,

Kubala³

et al. 2013

Plant Prot. Sci.

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The readthrough domain of pea enation mosaic virus coat protein is not essential for virus stability in the hemolymph of the pea aphid

et al. 2009

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A fraction of the coat protein (CP) subunits in virions of members of the family Luteoviridae contain a C-terminal extension called the readthrough domain (RTD). The RTD is necessary for persistent aphid transmission, but its role is unknown. It has been reported to be required for virion stability in the hemolymph. Here, we tested whether this was the case for pea enation mosaic virus (PEMV) virions in the pea aphid (Acyrthosiphon pisum) using RNA1Delta, a natural deletion mutant lacking the middle portion of the RTD ORF, and CPDeltaRTD, in which the entire RTD ORF was deleted. In infected plants, RNA1Delta virions were as abundant and stable as wild-type (WT) virions, while CPDeltaRTD virions were unstable. No RTD of any size was translated from artificial subgenomic mRNA of CPDeltaRTD or RNA1Delta in vitro. Thus, only the major CP was present in the mutant virions. Using real-time RT-PCR to detect virion RNA, no significant differences in the concentration or stability of WT and RNA1Delta virions were detected in the aphid hemolymph at much longer times than are necessary for virus transmission. Thus, the RTD is not necessary for stability of PEMV RNA in the aphid hemolymph, and it must play another role in aphid transmission.

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Genetic variability of the Czech Pea enation mosaic virus isolates

Šafářová¹,

Navrátil²

2014

CZECH J GENET PLANT

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AbstractŠafářová D., Navrátil M. (2014): Genetic variability of the Czech Pea enation mosaic virus isolates. Czech J. Genet. Plant Breed., 50: 100-104.The Pea enation mosaic virus is an example of symbiogenesis of taxonomically unrelated PEMV-1 and PEMV-2 viruses. Partial RNA sequences, PEMV-1 coat protein, PEMV-2 movement protein gene and satellite RNA from seventeen isolates were compared with the aim of enlarging the knowledge of PEMV variability. The isolates showed genetic variability based on the nucleotide sequences in both RNA1 and RNA2, with 98-94% identity within coat protein gene sequences, and 95-96% identity within the movement protein. The phylogenetic analyses showed different evolution of both symbiotic viruses and differences in the European isolates. Most of the differences seen were synonymous without changes in the structure of proteins. The analysis of satellite RNA positive isolates implies a possible correlation between the structure of coat protein gene and presence of RNA3.

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Comparison of Pea Enation Mosaic Virus (PEMV) Isolates: Sequence of Coat Protein and Aphid Transmission Protein Genes of a European Isolate

Cited by 3 publications

References 14 publications

Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus

Reactive oxygen and nitrogen species and hormone signalling in systemic infection of pea by Pea enation mosaic virus

The readthrough domain of pea enation mosaic virus coat protein is not essential for virus stability in the hemolymph of the pea aphid

Genetic variability of the Czech Pea enation mosaic virus isolates

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