Comparison of ovalbumin and ovalbumin epitope peptide for transdermal delivery and vaccination mediated by the photothermal effect of gold nanorods

Abstract

Transdermal protein delivery is a powerful and attractive method for protein therapy and dermal vaccination compared with other administrations. However, this delivery method is restricted by the low permeability of the stratum corneum (SC), a hydrophobic barrier that restricts the entry of hydrophilic molecules such as proteins. In this study, we developed an improved gel patch system carrying ovalbumin and ovalbumin epitope peptide, and then compared their permeability into the skin. First, the gel patch was placed on mouse skin to allow contact with the polymer coated gold nanorods and then irradiated by a continuous-wave laser. Thermal ablation of the SC improved the permeability and translocation of ovalbumin and the peptide. Fluorescence images showed the translocation was enhanced when the skin was treated with the FITC-modified ovalbumin epitope peptide. However, induction of anti-OVA IgG production after treatment with the FITC-modified ovalbumin epitope peptide was lower than that with FITC-OVA.

Research progress of physical transdermal enhancement techniques in tumor therapy

“…and carbon NPs have been used for TDD because of their advantages of nearinfrared (NIR) absorption, adjustable size, large surface area-to- volume ratio, easy synthesis, and surface functionalization. [92][93][94][95][96] Haine et al demonstrated the feasibility of transdermal delivery of ovalbumin (OVA) and OVA epitope peptides mediated by the photothermal effect of PTET in Au nanorods. 94 Similarly, PTET in hollow CuS NPs developed by Lu's group 97 achieved good results.…”

“…[92][93][94][95][96] Haine et al demonstrated the feasibility of transdermal delivery of ovalbumin (OVA) and OVA epitope peptides mediated by the photothermal effect of PTET in Au nanorods. 94 Similarly, PTET in hollow CuS NPs developed by Lu's group 97 achieved good results. Some metal-doped NPs, such as NPs with a metal framework and NPs derived from polymerization of small molecules (PDA, 2D phosphorene materials), also have high photothermal conversion efficiency and disease treatment ability, which are expected to be successfully used in PTET.…”