Comparison of Osteogenic Potential Between Apatite-Coated Poly(Lactide-Co-Glycolide)/Hydroxyapatite Particulates and Bio-Oss

Kim, Sang Soo; Kim, Byung Soo

doi:10.4012/dmj.27.368

Cited by 24 publications

(16 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Skeletally mature rats (We use inbred Fisher 344 rats, 11–12 week old, male, Harlan, catalog number F344/NHsd; however, inbred Lewis 34 and outbred Sprague-Dawley 28,30,35 and Wistar 25 rats have been used. )…”

Section: Methodsmentioning

confidence: 99%

Evaluation of bone regeneration using the rat critical size calvarial defect

et al. 2012

View full text Add to dashboard Cite

Animal models that are reliably reproducible, appropriate analogues to the clinical condition they are used to investigate, and that offer minimal morbidity and periprocedural mortality to the subject are the keystone to the preclinical development of translational technologies. For bone tissue engineering, a number of small animal models exist. Here we describe the protocol for one such model, the rat calvarial defect. This versatile model allows for evaluation of biomaterials and bone tissue engineering approaches within a reproducible, nonload-bearing orthotopic site. Critical steps to ensure appropriate experimental control and troubleshooting tips learned through extensive experience with this model are provided. The surgical procedure itself takes approximately 30 minutes to complete with approximately 2 hours of perioperative care, and tissue harvest is generally performed 4 to 12 weeks postoperatively. Several analytical techniques are presented, which evaluate the cellular and extracellular matrix components, functionality and mineralization, including histological, mechanical and radiographic methods.

show abstract

Section: Methodsmentioning

confidence: 99%

Evaluation of bone regeneration using the rat critical size calvarial defect

et al. 2012

View full text Add to dashboard Cite

show abstract

“…This same study also showed that the nanocrystal hydroxyapatite easily adhered to the fibroblasts and osteoblasts of the periodontal ligament (17). Porosity, surface structure, particle size and chemical features have are key characteristics that dictate the regenerative and osteoconductive potential of bone substituents (18). Thus, the greater treatment efficacy of Biohapel that we observed was most likely a consequence of the specific features of the material.…”

Section: Discussionmentioning

confidence: 57%

Evaluaton of the Use of Bone Implants as a Therapy for Deep Defects in the Parodoncium

Stevanović

Ciric

2014

Serbian Journal of Experimental and Clinical Research

View full text Add to dashboard Cite

Reconstruction of infrabony defects created by the chronic inflammatory processes of parodontopathy represents a major clinical problem in paradontology. The objective of this research was to compare and evaluate the efficiency of two bone substituents at reversing the deep infrabony defects in the paradentium after the application of a new “Biohapel” material consisting of biphasic calcium phosphate/poly DL-lactide-co-glycolide. This study included 12 patients of both genders with an average age of 49.7 years who were suffering from clinically manifested parodontopathy. The main criteria for selecting patients were the presence of six similar infrabony periodontal defects that were at least 4 mm deep and in the lower side of the teeth on both sides of the jaw. After applying a basic causal parodontopathy treatment, defects were divided into two groups, including an experimental group (n=72) in which defects were reconstructed using the “Biohapel” bone substituent (on one side of the lower jaw) and a control group (n=72) in which infrabony defects were reconstructed using β tricalcium phosphate (βTCP) (CerasorbR), a preparation very commonly applied in regenerative parodontopathy treatment (on the other side of the lower jaw). Markers to assess treatment efficacy were determined before surgery and 6 months after surgery by measuring the depth of periodontal pocket and the level of the junctional epithelium. We observed statistically significant differences in the periodontal regeneration markers between the experimental and control groups (p<0.05). Application of “Biohapel” during the surgical treatment of infrabony defects in the paradentium significantly improves the bone regeneration of the paradentium when compared with the standard application of β-tricalcium phosphate .

show abstract

“…The initially higher relative expression of Spp1 in the B group could be attributed to the osteoconductive nature of Bio-Oss Ò : it 'attracts' resident progenitors, [29] thus building a 'framework' for blood vessels and bone-forming cells. [30] However, the relative expression of Spp1 was more sustainable in EPB implants compared to PB and B ones, which might be related to interaction that occurs between secreted osteopontin and ECs. Soluble osteopontin has an anti-apoptotic effect on adherent ECs [31] and it can, therefore, be presumed that an impact on sustainable vascularization and osteogenic process in EPB implants can be achieved through this mechanism.…”

Section: Mm_vcam1_1_sg Qt00128793mentioning

confidence: 92%

Effects of bone tissue engineering triad components on vascularization process: comparative gene expression and histological evaluation in an ectopic bone-forming model

Najdanović

Cvetković

Stojanović

et al. 2016

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Vascular development has a great significance in the osteogenic process and in bone tissue engineering (BTE). BTE is based on various combinations of three principal types of components: biomaterials as scaffolds, regulatory signals and cells. The aim of our study was to evaluate, at gene expression and histological level, the effect of BTE triad components on the vascularization process in an ectopic bone-forming model in mice. Bone mineral matrix (BMM) was used as a scaffold and a carrier, platelet-rich plasma (PRP) as a source of regulatory signals and adipose stem cells (ASCs) as a source of cells for endothelial differentiation, in order to show how and to what extent the biological enrichment of BMM influences the outcome of the osteogenic process and its key precondition, vascularization. Implants composed of BMM, PRP and ASCs in vitro induced into endothelial cells (EPB implants) and implants composed of BMM and PRP (PB implants) were compared with implants composed of BMM only (B implants). More pronounced endothelialrelated gene expression and stronger VCAM-1 (vascular cell adhesion molecule-1) immunoexpression were observed in EPB implants in comparison with PB and B ones at later time points of the in vivo experimental period. Osteopontin gene expression and immunoexpression of osteopontin were significantly higher in EPB compared to PB and B implants. Therefore, addition of ASCs combined with PRP to BMM improved the vascularization process in the ectopic boneforming model, which makes this BTE composition the most favourable among the examined types of implants for application in BTE.

show abstract

Comparison of Osteogenic Potential Between Apatite-Coated Poly(Lactide-Co-Glycolide)/Hydroxyapatite Particulates and Bio-Oss

Cited by 24 publications

References 24 publications

Evaluation of bone regeneration using the rat critical size calvarial defect

Evaluation of bone regeneration using the rat critical size calvarial defect

Evaluaton of the Use of Bone Implants as a Therapy for Deep Defects in the Parodoncium

Effects of bone tissue engineering triad components on vascularization process: comparative gene expression and histological evaluation in an ectopic bone-forming model

Contact Info

Product

Resources

About