Comparison of osteogenic differentiation potential of human adult stem cells loaded on bioceramic‐coated electrospun poly (L‐lactide) nanofibres

Ardeshirylajimi, Abdolreza; Mossahebi-Mohammadi, Majid; Vakilian, Saeid; Langroudi, Ladan; Seyedjafari, Ehsan; Atashi, Amir; Soleimani, Masoud

doi:10.1111/cpr.12156

Cited by 57 publications

(54 citation statements)

References 42 publications

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“…BFPSCs in comparison to ADSCs from other sources have shown similar efficacy in differentiation and adherence to collagen membrane and polyglycolic acid . Moreover, similar expression level of RUNX‐2, osteopontin, osteocalcin, and ALP activity have been detected in BFPSCs, compared to bone marrow MSC . Our in vitro findings also confirmed BFPSCs' osteogenic potential.…”

Section: Discussionsupporting

confidence: 80%

Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

Khojasteh

Hosseinpour

Rad

et al. 2019

Clin Implant Dent Rel Res

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Background Application of adipose‐derived stem cells originated from buccal fat pad (BFP) can simplify surgical procedures and diminish clinical risks compared to large autograft harvesting. Purpose This study sought to evaluate and compare the efficacy of buccal fat pad‐derived stem cells (BFPSCs) in combination with anorganic bovine bone mineral (ABBM) for vertical and horizontal augmentation of atrophic posterior mandibles. Materials and Methods Fourteen patients with atrophic posterior mandible were elected for this prospective exploratory study. BFP (3‐5 mL) was harvested and BFPSCs were isolated and combined with ABBM at 50% ratio. The vertical and horizontal alveolar deficiencies were augmented by 50% mixture of ABBM with either BFPSCs (group 1) or particulated autologous bone (group 2). Titanium mesh was contoured to the desired 3D shape of the alveolar ridge and fixated to the host sites over the graft material of the two groups. At first, the amount of new bone areas was calculated by quantitative analysis of cone beam computed tomography (CBCT) images that were taken 6 months postoperatively according to regenerative techniques (group 1 vs group 2 without considering the type of bone defects). Second, these amounts were calculated in each group based on the type of defects. Results Quantitative analysis of CBCT images revealed the areas of new bone formation were 169.5 ± 5.90 mm2 and 166.75 ± 10.05 mm2 in groups 1 and 2, respectively. The area of new bone formation for vertical defects were 164.91 ± 3.74 mm2 and 169.36 ± 12.09 mm2 in groups 1 and 2, respectively. The area of new bone formation for horizontal deficiencies were 170.51 ± 4.54 mm2 and 166.98 ± 9.36 mm2 in groups 1 and 2, respectively. There were no statistically significant differences between the two groups in any of the pair‐wise comparisons (P > 0.05). Conclusions The findings of the present study demonstrated lack of difference in bone volume formation between BFPSCs and autologous particulate bone in combination with ABBM. If confirmed by future large‐scale clinical trial, BFPSCs may provide an alternative to autogenous bone for reconstruction of alveolar ridge defects.

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Section: Discussionsupporting

confidence: 80%

Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

Khojasteh

Hosseinpour

Rad

et al. 2019

Clin Implant Dent Rel Res

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“…Bio-Oss is a deproteinized bovine bone mineral used to treat bone defects, dental implant therapy and healing for alveolar process defects. We also showed, in two previous studies, that Bio-Oss could significantly increase osteogenic differentiation potential of MSCs in vitro and bone regeneration in vivo (23,46). BioOss has a highly porous structure (75% -80 % of the total volume) that increases surface area of the scaffold (47).…”

Section: Discussionsupporting

confidence: 68%

Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic-Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats

Sanchooli

Norouzian

Ardeshirylajimi

et al. 2017

Iran Red Crescent Med J

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Background: Tissue engineering is an available treatment for large bone defects. The therapeutic effects of mesenchymal stem cell (MSC) are mostly attributed to secretion of many cytokines and growth factors. Many factors of MSC secretions accumulate in a conditioned medium and these factors recruit native cells into a defect site to generate new bone tissues. Objectives: The aim of this study was to evaluate the influence of adipose tissue derived MSCs-conditioned medium (ADMSC-CM) on bone repair of rats with critical -size calvarial defect. Methods: This experimental study was performed at Shahid Beheshti University of Medical Sciences, Tehran, Iran, from 2016 to 2017. Conditioned medium was collected from healthy rat adipose tissue derived MSC (ADMSC) at passage four. Calvarial bone defect was created in hypothyroid rats using a dental bur. Sampling was taken by the linear-mono-gram method to determine sample size (n = 6 per group). The rats were divided randomly into four groups based on graft material as follows: empty defect, scaffold (Bio-Oss / type I collagen gel), scaffold / ADMSCs, scaffold /ADMSC-CM. Evaluations were made at 4 and 8 weeks after surgery using stereological analysis. Results: Histological analysis at 8 weeks indicated that the newly regenerated tissue almost covered the defect in the ADMSC-CM group. Stereological analysis showed that ADMSC-CM increased regenerated bone and numbers of osteocytes and osteoblasts compared with the defect and scaffold groups (P < 0.05). Also, bone regeneration was more effective in animals treated with ADMSC-CM than in those received ADMSC. Conclusions: These results suggest an important role for ADMSC-CM in bone regeneration, through trophic impact of its cytokines and growth factors that induce native cell proliferation and migration into the defect. Thus, ADMSC-CM seems to have good potential for application in bone tissue regeneration, in the cases of hypothyroidism.

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“…The oral cavity in humans encompasses a mass of especial fatty tissue, the buccal fat pad (BFP), which is another source of stem cells (Farre‐Guasch, Marti‐Page, Hernadez‐Alfaro, Klein‐Nulend, & Casals, ). Recently new type of MSCs derived from BFP, which is an adipose‐encapsulated mass of the oral cavity (Ardeshirylajimi, Mossahebi‐Mohammadi, et al, ). Different studies have investigated the potential of MSC derived‐BFP in the in vitro and in vivo condition for cell therapy and tissue applications.…”

Section: Stem Cellsmentioning

confidence: 99%

Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds

Moradi

Golchin

Hajishafieeha

et al. 2018

Journal Cellular Physiology

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Since bone tissue lesions caused by several reasons and has global outbreak without any attentions to the modernity level of the countries. In the other hands treatment of patients with this problem faced to the several limitations, in this because the future of the bone lesions treatments is related to the future of the bone tissue engineering. This review tries to cover the most suitable stem cells and materials from either natural or synthetic sources for bone tissue engineering. These understanding points would help researchers to further uncover the application of different adult stem cell sources in electrospinning scaffolds, promotion of nanofibrous composite construct design and adult stem cell type selection to enhance cell function and bone tissue engineering, and link laboratory investigations to clinical applications.

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Comparison of osteogenic differentiation potential of human adult stem cells loaded on bioceramic‐coated electrospun poly (L‐lactide) nanofibres

Cited by 57 publications

References 42 publications

Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

Adipose Derived Stem Cells Conditioned Media in Combination with Bioceramic-Collagen Scaffolds Improved Calvarial Bone Healing in Hypothyroid Rats

Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds

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