Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds

Moradi, Sadegh Lotfalah; Golchin, Ali; Hajishafieeha, Zahra; Khani, Mohammad‐Mehdi; Ardeshirylajimi, Abdolreza

doi:10.1002/jcp.26606

Cited by 73 publications

(45 citation statements)

References 114 publications

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“…84,85 This technique has been applied to prepare biomimetic and basic scaffolds from a variety of natural and synthetic biomaterials, such as Col, CTS, cellulose, HA, PLA, PC, and PCL/PLA blend solution. 84,86 It has been shown that biomimetic electrospun HA/Col/CTS nanofibers could promote the osteogenic differentiation and bone regeneration in mouse cranial bone defect models. This biomimetic nanofiber system was prepared by first making a HA/CTS (30:70, w/w) nanocomposite via the coprecipitation, and then doping it with Col to provide multicomponent solution for electrospinning.…”

Section: Electrospinningmentioning

confidence: 99%

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

2020

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Natural bone is a mineralized biological material, which serves a supportive and protective framework for the body, stores minerals for metabolism, and produces blood cells nourishing the body. Normally, bone has an innate capacity to heal from damage. However, massive bone defects due to traumatic injury, tumor resection, or congenital diseases pose a great challenge to reconstructive surgery. Scaffold-based tissue engineering (TE) is a promising strategy for bone regenerative medicine, because biomaterial scaffolds show advanced mechanical properties and a good degradation profile, as well as the feasibility of controlled release of growth and differentiation factors or immobilizing them on the material surface. Additionally, the defined structure of biomaterial scaffolds, as a kind of mechanical cue, can influence cell behaviors, modulate local microenvironment and control key features at the molecular and cellular levels. Recently, nano/micro-assisted regenerative medicine becomes a promising application of TE for the reconstruction of bone defects. For this reason, it is necessary for us to have in-depth knowledge of the development of novel nano/micro-based biomaterial scaffolds. Thus, we herein review the hierarchical structure of bone, and the potential application of nano/micro technologies to guide the design of novel biomaterial structures for bone repair and regeneration.

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Section: Electrospinningmentioning

confidence: 99%

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

2020

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“…1 One of the most important parts of the tissue engineering is the scaffold, which can simulate ECM during stem cell differentiation process in vitro and in vivo. 3,4 Among the tens scaffolds that have been introduced for use in tissue engineering for more than two decades, polycaprolactone (PCL) and poly (L-lactic acid) (PLLA) are the most appropriate of them for bone tissue engineering. The mechanical property of the scaffolds is a very important criterion.…”

Section: Introductionmentioning

confidence: 99%

Promoting osteogenic differentiation of human‐induced pluripotent stem cells by releasing Wnt/β‐catenin signaling activator from the nanofibers

Hosseini

Soleimanifar

Khojasteh

et al. 2018

J of Cellular Biochemistry

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Implants that can enhance the stem cells differentiation in the absence of the chemical osteogenic growth factors will attract the great interest of orthopedic scientists. Inorganic polyphosphate (poly‐P), as a ubiquitous biological polymer, is one of the factors that can be an alternative for osteogenic growth factors via activating Wnt/β‐catenin signaling. In this study, poly‐P was incorporated at the blend of polycaprolactone (PCL)/poly (l‐lactic acid) (PLLA) electrospun nanofibers and then osteogenic differentiation potential of human‐induced pluripotent stem cells (iPSCs) was investigated by the important bone markers. 3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5 diphenyl tetrazolium bromide (MTT) and scanning electron microscopy results confirmed the biocompatibility of the fabricated nanofibers, while higher proliferation rate of iPSCs was detected in PCL‐PLLA(poly‐P) group compared with the PCL‐PLLA and tissue culture plate groups. Alkaline phosphatase activity, calcium content, and gene expression results demonstrated that osteogenic differentiation of iPSCs was increased when cultured on PCL‐PLLA(poly‐P) in comparison with other groups. According to the results, PCL‐PLLA(poly‐P) could be considered as a promising candidate for use as bone implants.

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“…Many different cell types including primary human bone and periosteal cells and stem cells play a crucial role in the bone regeneration process. Especially, mesenchymal stem cells (MSCs), which have osteogenic differentiation capability, are promising candidates for bone regeneration therapies due to their easy isolation from different tissues, rapid proliferation, unique immunomodulatory properties [18][19][20]. Researchers have developed a range of MSCs-based electrospun scaffolds possessing highly interconnected pore architecture with the nano-and microscale size, which provide good interaction between the MSCs and fibrous scaffolds.…”

Section: Introductionmentioning

confidence: 99%

Stem cells combined 3D electrospun nanofibrous and macrochannelled matrices: a preliminary approach in repair of rat cranial bones

İşoğlu

Bölgen

Korkusuz

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Repair of cranial bone defects is an important problem in the clinical area. The use of scaffolds combined with stem cells has become a focus in the reconstruction of critical-sized bone defects. Electrospinning became a very attracting method in the preparation of tissue engineering scaffolds in the last decade, due to the unique nanofibrous structure of the electrospun matrices. However, they have a limitation for three dimensional (3D) applications, due to their two-dimensional structure and pore size which is smaller than a cellular diameter which cannot allow cell migration within the structure. In this study, electrospun poly(e-caprolactone) (PCL) membranes were spirally wounded to prepare 3D matrices composed of nanofibers and macrochannels. Mesenchymal stromal/stem cells were injected inside the scaffolds after the constructs were implanted in the cranial bone defects in rats. New bone formation, vascularisation and intramembranous ossification of the critical size calvarial defect were accelerated by using mesenchymal stem cells combined 3D spiral-wounded electrospun matrices.

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Bone tissue engineering: Adult stem cells in combination with electrospun nanofibrous scaffolds

Cited by 73 publications

References 114 publications

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

Effect of the nano/microscale structure of biomaterial scaffolds on bone regeneration

Promoting osteogenic differentiation of human‐induced pluripotent stem cells by releasing Wnt/β‐catenin signaling activator from the nanofibers

Stem cells combined 3D electrospun nanofibrous and macrochannelled matrices: a preliminary approach in repair of rat cranial bones

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