Comparison of OspA Serotypes for <i>Borrelia burgdorferi</i> Sensu Lato from Japan, Europe and North America

Masuzawa, Toshiyuki; Wilske, Bettina; Komikado, Tetsuro; Suzuki, Hiroyuki; Kawabata, Hiroki; Sato, Nanao; Muramatsu, Ken; Sato, Nobutake; Isogai, Emiko; Isogai, Hiroshi; Johnson, Russell C.; Yanagihara, Yasutake

doi:10.1111/j.1348-0421.1996.tb01106.x

Cited by 37 publications

(43 citation statements)

References 41 publications

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“…garinii has been previously reported (6,20,(28)(29)(30). This heterogeneity might explain the wide vector tolerance of B. garinii for the two closely related tick species and I. persulcatus (6), and adaptation to birds and small rodents (22,23).…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Borrelia burgdorferi Sensu Lato Isolated in Far Eastern Russia

Masuzawa

Iwaki

Sato

et al. 1997

Microbiology and Immunology

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One-hundred and fifty-seven Borrelia isolated from adult ticks, Ixodes persulcatus, and wild rodents, Clethrionomys rufocanus and Apodemus peninsulae, in the far eastern part of Russia were charaeterized and identified by restriction fragment length polymorphism (RFLP) of the 5S-23S rRNA intergenic spacer. Some isolates showed unique RFLP patterns and were determined as Borrelia garinii on the basis of a sequence analysis of the intergenic spacer amplicon and reactivity with species-specific monoclonal antibodies (MAbs). 86.5 and 12.7% of the tick isolates, and 74.2 and 12.9% of the rodent isolates were determined as Borrelia garinii and Borrelia afzelii, respectively, but no Borrelia burgdorferi sensu stricto was detected. This finding is similar to the results obtained from Borrelia surveys of I. persulcatus and wild rodents in Hokkaido, Japan.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Borrelia burgdorferi Sensu Lato Isolated in Far Eastern Russia

Masuzawa

Iwaki

Sato

et al. 1997

Microbiology and Immunology

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“…Given the higher sensitivity and the possibility of storing the slides for several months, the direct or IFA can be considered as the method of choice to determine Borrelia infection in ticks. In principle, it allows the specific detection of Borrelia genospecies and detection of outer-surface protein expression when appropriate monoclonal antibodies are applied (Wilske et al, 1993Fingerle et al, 1995 ;Masuzawa et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Visualization of Borrelia burgdorferi sensu lato by fluorescence in situ hybridization (FISH) on whole-body sections of Ixodes ricinus ticks and gerbil skin biopsies

et al. 2001

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The objective of this study was to visualize borreliae directly in whole-body sections of Ixodes ricinus by fluorescence in situ hybridization (FISH). Borrelia afzelii mono-infected or Borrelia burgdorferi sensu stricto (ss)/B. afzelii doubleinfected nymphs were fixed, embedded in cold polymerizing resin and sectioned. The same sample processing was applied to skin biopsies taken from a Mongolian gerbil after an infectious tick-bite. FISH was carried out using 16S-rRNA-directed, fluorescence-labelled oligonucleotide probes specific for the genus Borrelia and specific within the group of Lyme borreliosisassociated genospecies B. afzelii, B. burgdorferi ss, Borrelia garinii and Borrelia valaisiana. Sensitivity and specificity of the newly designed probes were evaluated using PCR, dot-blot hybridizations and FISH. Despite significant autofluorescence of certain tick tissues (which allowed good histological orientation within the sections), borreliae showing the typical spirochaetal morphotype were clearly visible in five out of six putatively infected ticks. These findings were confirmed by electron microscopy of ticks from the same infected batch as used for FISH. Attempts to produce ticks infected by two different Borrelia genospecies were not successful. FISH on whole-body sections of resin-embedded ticks offers the possibility of visualizing and identifying borreliae within tick tissues. This technique has great potential for the investigation of the transmission of bacteria or other micro-organisms by arthropod vectors. Furthermore, clear visualization of single spirochaetes distributed along subcutaneous fat cell membranes in gerbil skin biopsies suggests that FISH might also be suitable for the detection of borreliae in clinical tissue specimens.

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“…However, the high degree of variability of OspA has been well documented. The OspA of strains belonging to B. burgdorferi sensu stricto, B. afzelii and B. japonica are homologous within species, but not among those of strains belonging to B. garinii isolated in Europe (22,30,32,33). On the other hand, it has been demonstrated that vaccination with recombinant OspC protected gerbils from experimental challenge with a Borrelia (26).…”

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“…We previously showed that Japanese isolates (OspA serotype J1 to J9) identified as B. garinii were antigenically different from European B. garinii isolates (OspA serotypes 3 to 7) by the OspA serotyping system (22,33). No cross-protection was obtained with a heterologous vaccine prepared using isolates from Europe and…”

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“…The borrelial strains used in this study (Table 1) were cultivated at 33 C in Barbour-Stoenner-Kelly II (BSK II) medium (3). Challenge strains used in this study were B. burgdorferi strain 297, B. afzelii strain PGau, B. garinii strains Ir210, HP1 and NP81 which had been maintained by passage in five-week-old ddY mice (SLC, Hamamatsu, Japan) and cultured in BSK II at 33 C. Strains used for vaccine preparation (Table 1) were chosen based on previously determined OspA serotypes (22,33). Cells were washed by centrifugation and resuspended in distilled water.…”

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Negative Finding in Cross‐Protective Activity of JapaneseBorreliaIsolates against Infection with Three Species of Lyme DiseaseBorreliain Outbred Mice

Masuzawa

Kurita

Yanagihara

1997

Microbiology and Immunology

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Outer surface protein A (OspA) is the most promising candidate for a component vaccine against Lyme disease caused by Borrelia burgdorferi sensu lato. Active cross-protection using a whole-cell vaccine prepared from strains belonging various OspA serotypes observed in Japan and worldwide was examined. No cross-protection was obtained by heterologous OspA-serotype vaccines. Since OspA is a highly variable protein expressed by Borrelia, this suggests that immunologically different OspA serotypes need to be combined for the development of an effective vaccine in Japan.

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Comparison of OspA Serotypes for Borrelia burgdorferi Sensu Lato from Japan, Europe and North America

Abstract: 1.B. burgdorferi sensu stricto has not been observed in Japan, 2. Japanese B. afzelii isolates are closely related to those from Europe, 3. B. garinii isolates from Japan are highly heterogeneous and apparently different from European B. garinii isolates.

Cited by 37 publications

References 41 publications

Genetic Diversity of Borrelia burgdorferi Sensu Lato Isolated in Far Eastern Russia

Genetic Diversity of Borrelia burgdorferi Sensu Lato Isolated in Far Eastern Russia

Visualization of Borrelia burgdorferi sensu lato by fluorescence in situ hybridization (FISH) on whole-body sections of Ixodes ricinus ticks and gerbil skin biopsies

Negative Finding in Cross‐Protective Activity of JapaneseBorreliaIsolates against Infection with Three Species of Lyme DiseaseBorreliain Outbred Mice

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