Comparison of new giant bacteriophages OBP and Lu11 of soil pseudomonads with bacteriophages of the ϕKZ-supergroup of Pseudomonas aeruginosa

Shaburova, O. V.; Hertveldt, Kirsten; Cruz, D. M. A de la; Krylov, S. V.; Плетенева, Е. А.; Bourkaltseva, Maria; Lavigne, Rob; Volckaert, Guido; Крылов, В. Н.

doi:10.1134/s1022795406080059

Cited by 15 publications

(14 citation statements)

References 12 publications

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“…Shaburova et al (47) reported that the giant virulent bacteriophage OBP, isolated from a compost sample in the Tver district (Russia, 2001), forms small turbid plaques on its bacterial host, Pseudomonas fluorescens Pf 1.1. Using transmission electron microscopic imaging, they revealed that OBP belongs to morphogroup A1 of the family Myoviridae of double-stranded DNA bacteriophages (119-nm head; 191-nm contractile tail).…”

Section: Resultsmentioning

confidence: 99%

“…Unique to the KZ-related phages is the cylindrical inner body observed within the phage capsid (31)(32)(33)47). This inner body is thought to be a dense spiral structure around which DNA molecules are spooled.…”

Section: Figmentioning

confidence: 99%

“…seudomonas fluorescens phage OBP (vB_PflM_OBP) (30,47) is member of a growing group of giant phages, isolated, to date, only from Pseudomonas species and represented by the completely sequenced and well-studied Pseudomonas aeruginosa myovirus KZ (9, 18, 19, 31-33, 36, 42). Three other phages encoding many proteins with similarity to KZ proteins have been completely sequenced: EL (23), 2012-1 (52), and PA3 (43).…”

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confidence: 99%

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Complete Genome Sequence of the Giant Virus OBP and Comparative Genome Analysis of the Diverse ϕKZ-Related Phages

Cornelissen

Hardies

Shaburova

et al. 2012

J Virol

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The 283,757-bp double-stranded DNA genome of Pseudomonas fluorescens phage OBP shares a general genomic organization with Pseudomonas aeruginosa phage EL. Comparison of this genomic organization, assembled in syntenic genomic blocks interspersed with hyperplastic regions of the KZ-related phages, supports the proposed division in the "EL-like viruses," and the "phiKZ-like viruses" within a larger subfamily. Identification of putative early transcription promoters scattered throughout the hyperplastic regions explains several features of the KZ-related genome organization (existence of genomic islands) and evolution (multi-inversion in hyperplastic regions). When hidden Markov modeling was used, typical conserved core genes could be identified, including the portal protein, the injection needle, and two polypeptides with respective similarity to the 3=-5= exonuclease domain and the polymerase domain of the T4 DNA polymerase. While the N-terminal domains of the tail fiber module and peptidoglycan-degrading proteins are conserved, the observation of C-terminal catalytic domains typical for the different genera supports the further subdivision of the KZ-related phages. P seudomonas fluorescens phage OBP (vB_PflM_OBP) (30, 47) is member of a growing group of giant phages, isolated, to date, only from Pseudomonas species and represented by the completely sequenced and well-studied Pseudomonas aeruginosa myovirus KZ (9,18,19,[31][32][33]36,42). Three other phages encoding many proteins with similarity to KZ proteins have been completely sequenced: EL (23), 2012-1 (52), and PA3 (43). Krylov et al. (33) assigned 19 unsequenced Pseudomonas phages to this group. We refer to the group as KZ-related phages. Lavigne et al. (35) have argued that EL should be classified as a genus separate from KZ and 2012-1 based on its less extensive levels of similarity. By that criterion, PA3 belongs to the "phiKZ-like viruses" and the results reported here classify OBP as the second member of the possible genus EL-like viruses.OBP shares a number of definitive properties with the other KZ-related phages. Typically, KZ-related phages have a very large icosahedral head, ϳ122 nm in diameter, and a long (ϳ190-nm) contractile tail surrounded by fibers. A KZ-related phage head contains a proteinic inner body, which has been speculated to organize the packaged DNA (18, 32). The exceptionally large genomes of the KZ-related phages (between 211 and 317 kb of nonredundant sequence) are composed of circularly permuted and terminally redundant linear double-stranded DNAs as determined for KZ and 2012-1 (42, 52). The KZ-related genomes display a pronounced difference in GϩC content (between 36.8 and 48%) and all have markedly lower GϩC content than the chromosomes of their GC-rich Pseudomonas hosts (60 to 66% GϩC).This group of phages has numerous genes involved in nucleotide metabolism (e.g., thymidylate synthase, thymidylate kinase, ribonucleoside diphosphate reductase, subunit beta [NrdB], and dihydrofolate reductase) and at least six genes enco...

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Section: Resultsmentioning

confidence: 99%

Section: Figmentioning

confidence: 99%

mentioning

confidence: 99%

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Complete Genome Sequence of the Giant Virus OBP and Comparative Genome Analysis of the Diverse ϕKZ-Related Phages

Cornelissen

Hardies

Shaburova

et al. 2012

J Virol

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“…The closest relative of PaBG is Pseudomonas putida phage Lu11 (12, 13). One hundred twenty-five ORFs of these phages are similar on the protein level.…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequence of the Novel Giant Pseudomonas Phage PaBG

et al. 2014

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“…The host of this phage is Pseudomonas putida variant Manila, a common rhizosphere bacterium. We previously described the morphology and proteome of this virus (13). Briefly, Lu11 belongs to the Myoviridae family, with an icosahedral head of 124 nm and a contractile tail 200 nm in length and 20 nm wide.…”

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Complete Genome Sequence of the Giant Pseudomonas Phage Lu11

Adriaenssens

Mattheus

Cornelissen

et al. 2012

J Virol

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The complete genome sequence of the giant Pseudomonas phage Lu11 was determined, comparing 454 and Sanger sequencing. The double-stranded DNA (dsDNA) genome is 280,538 bp long and encodes 391 open reading frames (ORFs) and no tRNAs. The closest relative is Ralstonia phage RSL1, encoding 40 similar proteins. As such, Lu11 can be considered phylogenetically unique within the Myoviridae and indicates the diversity of the giant phages within this family.

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Comparison of new giant bacteriophages OBP and Lu11 of soil pseudomonads with bacteriophages of the ϕKZ-supergroup of Pseudomonas aeruginosa

Cited by 15 publications

References 12 publications

Complete Genome Sequence of the Giant Virus OBP and Comparative Genome Analysis of the Diverse ϕKZ-Related Phages

Complete Genome Sequence of the Giant Virus OBP and Comparative Genome Analysis of the Diverse ϕKZ-Related Phages

Complete Genome Sequence of the Novel Giant Pseudomonas Phage PaBG

Complete Genome Sequence of the Giant Pseudomonas Phage Lu11

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