Complete Genome Sequence of the Giant Pseudomonas Phage Lu11

Adriaenssens, Evelien M.; Mattheus, Wesley; Cornelissen, Anneleen; Shaburova, O. V.; Крылов, В. Н.; Kropinski, Andrew M.; Lavigne, Rob

doi:10.1128/jvi.00641-12

Cited by 24 publications

(24 citation statements)

References 12 publications

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“…They are all phages that infect Pseudomonas spp , phage Lu11 [61], phage vB_PaeS_PMG1 (NC_016765.1) and phage D3 [62]; two of which encode predicted integrases.…”

Section: Discussionmentioning

confidence: 99%

What Does the Talking?: Quorum Sensing Signalling Genes Discovered in a Bacteriophage Genome

2014

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The transfer of novel genetic material into the genomes of bacterial viruses (phages) has been widely documented in several host-phage systems. Bacterial genes are incorporated into the phage genome and, if retained, subsequently evolve within them. The expression of these phage genes can subvert or bolster bacterial processes, including altering bacterial pathogenicity. The phage phiCDHM1 infects Clostridium difficile, a pathogenic bacterium that causes nosocomial infections and is associated with antibiotic treatment. Genome sequencing and annotation of phiCDHM1 shows that despite being closely related to other C. difficile myoviruses, it has several genes that have not been previously reported in any phage genomes. Notably, these include three homologs of bacterial genes from the accessory gene regulator (agr) quorum sensing (QS) system. These are; a pre-peptide (AgrD) of an autoinducing peptide (AIP), an enzyme which processes the pre-peptide (AgrB) and a histidine kinase (AgrC) that detects the AIP to activate a response regulator. Phylogenetic analysis of the phage and C. difficile agr genes revealed that there are three types of agr loci in this species. We propose that the phage genes belonging to a third type, agr3, and have been horizontally transferred from the host. AgrB and AgrC are transcribed during the infection of two different strains. In addition, the phage agrC appears not to be confined to the phiCDHM1 genome as it was detected in genetically distinct C. difficile strains. The discovery of QS gene homologs in a phage genome presents a novel way in which phages could influence their bacterial hosts, or neighbouring bacterial populations. This is the first time that these QS genes have been reported in a phage genome and their distribution both in C. difficile and phage genomes suggests that the agr3 locus undergoes horizontal gene transfer within this species.

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“…They are all phages that infect Pseudomonas spp , phage Lu11 [61], phage vB_PaeS_PMG1 (NC_016765.1) and phage D3 [62]; two of which encode predicted integrases.…”

Section: Discussionmentioning

confidence: 99%

What Does the Talking?: Quorum Sensing Signalling Genes Discovered in a Bacteriophage Genome

2014

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“…P. aeruginosa , at least in other bacteria; similar phages were not found for a long time. However, as has been shown, bacteriophages of similar morphotype, showing signs of kinship with phiKZ, can be found in other bacterial hosts [48,51,111,112,113]. It is possible too that phiKZ-like phages will be found among other large phages of distant bacterial species.…”

Section: Possibilities For the Emergence Of New Phages In The Courmentioning

confidence: 95%

A Genetic Approach to the Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections

Крылов

Shaburova

Krylov

et al. 2012

Viruses

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Pseudomonas aeruginosa is a frequent participant in wound infections. Emergence of multiple antibiotic resistant strains has created significant problems in the treatment of infected wounds. Phage therapy (PT) has been proposed as a possible alternative approach. Infected wounds are the perfect place for PT applications, since the basic condition for PT is ensured; namely, the direct contact of bacteria and their viruses. Plenty of virulent (“lytic”) and temperate (“lysogenic”) bacteriophages are known in P. aeruginosa. However, the number of virulent phage species acceptable for PT and their mutability are limited. Besides, there are different deviations in the behavior of virulent (and temperate) phages from their expected canonical models of development. We consider some examples of non-canonical phage-bacterium interactions and the possibility of their use in PT. In addition, some optimal approaches to the development of phage therapy will be discussed from the point of view of a biologist, considering the danger of phage-assisted horizontal gene transfer (HGT), and from the point of view of a surgeon who has accepted the Hippocrates Oath to cure patients by all possible means. It is also time now to discuss the possible approaches in international cooperation for the development of PT. We think it would be advantageous to make phage therapy a kind of personalized medicine.

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“…The genome sequences of approximately 1,000 phages, isolated from a broad range of bacterial hosts, have been deposited in public databanks, and it is clear that we have only begun to appreciate the enormous sequence diversity of the phage population as a whole. For some bacterial genera, a substantial number of phage genome sequences have been determined, including Escherichia , Lactococcus , Mycobacterium , Pseudomonas , Staphylococcus , and Streptococcus , and these also typically span a broad range of diversity (Adriaenssens et al, 2012; Brussow, 2001; Hatfull, 2012b; Kwan et al, 2005, 2006; Smith et al, 2012). However, for many such collections, the phages were not isolated on the same species or strain, the host ranges are not well-defined, and there are many examples where phages have preferences for different strains of the same bacterial species (Lobocka et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

On the nature of mycobacteriophage diversity and host preference

et al. 2012

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The complete genome sequences of over 220 mycobacteriophages reveal them to be highly diverse, with numerous types sharing little or no nucleotide sequence identity with each other. We have determined the preferences of these phages for M. tuberculosis and for other strains of M. smegmatis, and find there is a correlation between genome type (cluster, subcluster, singleton) and host range. For many of the phages, expansion of host range occurs at relatively high frequencies, and we describe several examples in which host constraints occur at early stages of infection (adsorption or DNA injection), and phages have the ability to expand their host range through mutations in tail genes. We present a model in which phage diversity is a function of both the ability of phages to rapidly adapt to new hosts and the richness of the diversity of the bacterial population from which those phages are isolated.

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Complete Genome Sequence of the Giant Pseudomonas Phage Lu11

Cited by 24 publications

References 12 publications

What Does the Talking?: Quorum Sensing Signalling Genes Discovered in a Bacteriophage Genome

What Does the Talking?: Quorum Sensing Signalling Genes Discovered in a Bacteriophage Genome

A Genetic Approach to the Development of New Therapeutic Phages to Fight Pseudomonas Aeruginosa in Wound Infections

On the nature of mycobacteriophage diversity and host preference

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