1997
DOI: 10.1164/ajrccm.156.4.97-03017
|View full text |Cite
|
Sign up to set email alerts
|

Comparison of Murine Nasal-associated Lymphoid Tissue and Peyer's Patches

Abstract: The nasal mucosal is the first site of contact with inhaled antigens. However, the nature of local immune responses and the role of nasal-associated lymphoid tissue (NALT) in those responses have rarely been studied. To characterize the cells involved in mucosally derived immune responses, NALT and Peyer's patch (PP) cells from normal mice, and mice immunized intragastrically or intranasally with cholera toxin (CT), were isolated and analyzed. Compared with PP cells, unstimulated NALT cells contained a higher … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

7
108
2
4

Year Published

2002
2002
2018
2018

Publication Types

Select...
8
1

Relationship

0
9

Authors

Journals

citations
Cited by 122 publications
(121 citation statements)
references
References 30 publications
(24 reference statements)
7
108
2
4
Order By: Relevance
“…In contrast, orally inoculated mice had only a short persistence of vaccine organisms, which might be not sufficient to stimulate antibody responses in the lungs, and this likely accounted partially for the poor antibody responses against the heterologous antigen in this local tissue. The stronger persistence of vaccine organisms in the Peyer's patches may result from the environment, which is less hostile than that in either the lungs or the spleen of mice based on tissue specificity (13). Simultaneously, large numbers of organisms were isolated from the lungs, spleen, and Peyer's patches of mice following s.c. inoculation, suggesting that the s.c. inoculum may establish a reservoir in the lymph nodes, where Salmonella readily spreads throughout the body via the lymph stream and becomes systemic (5,35), finally reaching the lungs and other tissues for the generation of systemic and local immune responses.…”
Section: Discussionmentioning
confidence: 99%
“…In contrast, orally inoculated mice had only a short persistence of vaccine organisms, which might be not sufficient to stimulate antibody responses in the lungs, and this likely accounted partially for the poor antibody responses against the heterologous antigen in this local tissue. The stronger persistence of vaccine organisms in the Peyer's patches may result from the environment, which is less hostile than that in either the lungs or the spleen of mice based on tissue specificity (13). Simultaneously, large numbers of organisms were isolated from the lungs, spleen, and Peyer's patches of mice following s.c. inoculation, suggesting that the s.c. inoculum may establish a reservoir in the lymph nodes, where Salmonella readily spreads throughout the body via the lymph stream and becomes systemic (5,35), finally reaching the lungs and other tissues for the generation of systemic and local immune responses.…”
Section: Discussionmentioning
confidence: 99%
“…22 To obtain lymphoid cells without bone debris, NALTs were gently pushed off the palates and teased in medium with syringe needles and then the cells were passed through a 40-m Falcon cell strainer (BD Biosciences, Bedford, MA), isolated by Percoll (Amersham Biosciences, Piscataway, NJ), and washed with phosphate-buffered saline (PBS).…”
Section: Nalt Isolation and Cell Preparationmentioning
confidence: 99%
“…NALT cells were recovered for flow cytometric analysis and proliferation assays as previously described (5,21,24). Briefly, after exsanguination of the mice and isolation of cLN and spleen, the head and fore teeth were cut off.…”
Section: Nalt and Cell Preparationmentioning
confidence: 99%
“…However, there are no data available concerning the NALT. In contrast, the comparison between Peyer's patches and NALT demonstrated various differences, such as the fact that NALT contains a major population of naive T cells (1,21). An important question to be addressed is whether differences between NALT, bronchial-associated lymphoid tissues, and Peyer's patches reflect differences in the mechanisms involved in either the maintenance of local tolerance or the generation of immune responses.…”
mentioning
confidence: 99%