Subcutaneous Vaccination with Attenuated
 Salmonella enterica
 Serovar Choleraesuis C500 Expressing Recombinant Filamentous Hemagglutinin and Pertactin Antigens Protects Mice against Fatal Infections with both
 S. enterica
 Serovar Choleraesuis and
 Bordetella bronchiseptica

Zhao, Zhanqin; Xue, Yun; Wu, Bin; Tang, Xibiao; Hu, Ruiming; Xu, Yindi; Chen, Huanchun

doi:10.1128/iai.01495-07

Cited by 35 publications

(44 citation statements)

References 29 publications

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“…Niess and Sundquist have shown that, after oral vaccination, these attenuated bacteria could introduce the target DNA into the internal organs and lymph tissues, such as Peyers' patches, mesenteric lymph nodes, spleen, and liver . This strategy of bacterially delivered immunization has proven successful in inducing significant protection against infections . However, in our study, no evidence of integration after immunized inhibin vaccine was shown in any of investigated organs, suggesting the safety of this vaccine.…”

Section: Discussioncontrasting

confidence: 50%

“…Our previous studies have demonstrated that the attenuated crp À /asd À S. choleraesuis C500 strain could be utilized as a delivery system for foreign antigens by using the asd À balanced lethal host vector system [19]. The present study indicated that pVAX-asd-IS vector, containing recombinant inhibin a-subunit (1-32) fragment, could be used as potent enhancer of immunogenicity in mice.…”

Section: Discussionmentioning

confidence: 58%

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Oral vaccination with inhibin DNA delivered using attenuated Salmonella choleraesuis for improving reproductive traits in mice

Han

Zhen

Liang

et al. 2013

J. Basic Microbiol.

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The objective of this study was to examine the efficacy and safety of a novel inhibin vaccine containing inhibin α (1–32) fragments in mice. A recombinant plasmid pVAX‐asd‐IS was constructed by inserting recombinant inhibin α (1–32) and the hepatitis B surface antigen S into the plasmid in which the asd gene, rather than the kanamycin gene, was a selection marker. Ninety Kuming mice were divided into six groups consisting of 15 mice each. First group was (C1) injected with 200 µl of PBS, second (C2) received 1 × 1010 CFU of crp−/asd− C500/pVAX‐asd and served as vector control, third did not receive any treatment (C3), while fourth, fifth, and sixth group received 1 × 1010, 1 × 109, 1 × 108 CFU of the recombinant inhibin vaccine crp−/asd− C500/pVAX‐asd‐IS (group T1, T2, T3), respectively. Western blotting demonstrated that recombinant expressed inhibin protein possessed immune function and that this plasmid could replicate for up to 40 generations stably. Vaccination with this strain at a dose of 1 × 1010 CFU/200 µl per mouse induced high anti‐inhibin antibody levels, significantly increased large‐follicle production in T1 group (p < 0.05) and average litter size (p > 0.05) compared with control groups. Integration studies showed no evidence of inhibin fusion gene integrated into mice's genome 2‐month after immunization. These results suggest that the vaccine described in the present study may provide a safe method to improve reproductive traits in animals. A trend towards increased litter size and significant increase in large follicle population depict that this vaccine may have direct application in large animal industry.

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Section: Discussioncontrasting

confidence: 50%

Section: Discussionmentioning

confidence: 58%

Oral vaccination with inhibin DNA delivered using attenuated Salmonella choleraesuis for improving reproductive traits in mice

Han

Zhen

Liang

et al. 2013

J. Basic Microbiol.

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“…However, we had reason to believe that the ⌬asd mutant would be more effective than previous live attenuated strains. It has been shown that a more protective immune response can be achieved by increasing short-term vaccine persistence, which we attempted with the booster vaccine (5,23,45). Unfortunately, while vaccination with the ⌬asd mutant did indeed protect against acute melioidosis, the vaccine failed to protect against chronic melioidosis.…”

Section: Fig 6 (A)mentioning

confidence: 99%

The Burkholderia pseudomallei Δ asd Mutant Exhibits Attenuated Intracellular Infectivity and Imparts Protection against Acute Inhalation Melioidosis in Mice

et al. 2011

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Burkholderia pseudomallei, the cause of serious and life-threatening diseases in humans, is of national biodefense concern because of its potential use as a bioterrorism agent. This microbe is listed as a select agent by the CDC; therefore, development of vaccines is of significant importance. Here, we further investigated the growth characteristics of a recently created B. pseudomallei 1026b ⌬asd mutant in vitro, in a cell model, and in an animal model of infection. The mutant was typified by an inability to grow in the absence of exogenous diaminopimelate (DAP); upon single-copy complementation with a wild-type copy of the asd gene, growth was restored to wild-type levels. Further characterization of the B. pseudomallei ⌬asd mutant revealed a marked decrease in RAW264.7 murine macrophage cytotoxicity compared to the wild type and the complemented ⌬asd mutant. RAW264.7 cells infected by the ⌬asd mutant did not exhibit signs of cytopathology or multinucleated giant cell (MNGC) formation, which were observed in wild-type B. pseudomallei cell infections. The ⌬asd mutant was found to be avirulent in BALB/c mice, and mice vaccinated with the mutant were protected against acute inhalation melioidosis. Thus, the B. pseudomallei ⌬asd mutant may be a promising live attenuated vaccine strain and a biosafe strain for consideration of exclusion from the select agent list.

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“…One explanation to the higher IgG response after sc vaccination may be that bacteria trapped in skin are available to trigger the immune system, whereas part of bacteria via ig is killed by acidity in the stomach, or fail to bind PP and are washed away in feces. Similarly, Zhao and co-workers recently reported that the sc route provided higher protection than the oral route for mice vaccination with a live Salmonella vector producing Salmonella or Bordetella antigens [15].…”

Section: Resultsmentioning

confidence: 96%

Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine

Derbise

Guillas

Gerke

et al. 2019

Preprint

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A single oral inoculation to mice of the live attenuated Yersinia pseudotuberculosis VTnF1 strain producing an F1 pseudocapsule protects against bubonic and pneumonic plague.However oral vaccination can fail in humans exposed to frequent intestinal infections. We evaluated in mice the efficacy of subcutaneous vaccine injection as an alternative way to induce protective immunity, while reducing the dose and avoiding strain release in nature. A single subcutaneous dose of up to 10 8 CFU induced dose-dependent antibody production. At the dose of 10 7 CFU, i.e. 10 times less than via the oral route, it caused a modest skin reaction and protected 100% against bubonic and 80% against pneumonic plague, caused by high doses of Yersinia pestis. Bacteria migrating to lymph nodes and spleen, but not feces, were rapidly eliminated. Thus, subcutaneous injection of VTnF1 would represent a good alternative when dissemination in nature and human intestinal responsiveness are limitations.

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Subcutaneous Vaccination with Attenuated Salmonella enterica Serovar Choleraesuis C500 Expressing Recombinant Filamentous Hemagglutinin and Pertactin Antigens Protects Mice against Fatal Infections with both S. enterica Serovar Choleraesuis and Bordetella bronchiseptica

Abstract: Salmonella enterica serovar Choleraesuis strain C500 is a live, attenuated vaccine that has been used in China for over 40 years to prevent piglet paratyphoid. We compared the protective efficacies of subcutaneous (s.

Cited by 35 publications

References 29 publications

Oral vaccination with inhibin DNA delivered using attenuated Salmonella choleraesuis for improving reproductive traits in mice

Oral vaccination with inhibin DNA delivered using attenuated Salmonella choleraesuis for improving reproductive traits in mice

The Burkholderia pseudomallei Δ asd Mutant Exhibits Attenuated Intracellular Infectivity and Imparts Protection against Acute Inhalation Melioidosis in Mice

Subcutaneous vaccination with a live attenuated Yersinia pseudotuberculosis plague vaccine

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