Comparison of multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis in molecular subtyping of Salmonella enterica serovars Paratyphi A

Tien, Yung-Yen; Wang, You-Wun; Tung, Shu-Chu; Liang, Shiu-Yun; Chiou, Chien-Shun

doi:10.1016/j.diagmicrobio.2010.08.012

Cited by 20 publications

(11 citation statements)

References 21 publications

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“…MLVA is a genotyping technique widely used in clinical microbiology (36) that has a high level of discriminatory power (52,54). This technique has been compared with other typing techniques and its suitability demonstrated for intrapathovar analysis (3, 40a, 55).…”

Section: Discussionmentioning

confidence: 99%

Housekeeping Gene Sequencing and Multilocus Variable-Number Tandem-Repeat Analysis To Identify Subpopulations within Pseudomonas syringae pv. maculicola and Pseudomonas syringae pv. tomato That Correlate with Host Specificity

Gironde

Manceau

2012

Appl Environ Microbiol

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ABSTRACTPseudomonas syringaepv. maculicola causes bacterial spot onBrassicaceaeworldwide, and for the last 10 years severe outbreaks have been reported in the Loire Valley, France.P. syringaepv. maculicola resemblesP. syringaepv. tomato in that it is also pathogenic for tomato and causes the same types of symptoms. We used a collection of 106 strains ofP. syringaeto characterize the relationships betweenP. syringaepv. maculicola and related pathovars, paying special attention toP. syringaepv. tomato. Phylogenetic analysis ofgyrBandrpoDgene sequences showed thatP. syringaepv. maculicola, which causes diseases inBrassicaceae, forms six genetic lineages within genomospecies 3 ofP. syringaestrains as defined by L. Gardan et al. (Int. J. Syst. Bacteriol. 49[Pt 2]:469–478, 1999), whereasP. syringaepv. tomato forms two distinct genetic lineages. A multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) conducted with eight minisatellite loci confirmed the genetic structure obtained withrpoDandgyrBsequence analyses. These results provide promising tools for fine-scale epidemiological studies on diseases caused byP. syringaepv. maculicola andP. syringaepv. tomato. The two pathovars had distinct host ranges; onlyP. syringaepv. maculicola strains were pathogenic forBrassicaceae. A subpopulation ofP. syringaepv. maculicola strains that are pathogenic for Pto-expressing tomato plants were shown to lackavrPto1andavrPtoBor to contain a disruptedavrPtoBhomolog. Taking phylogenetic and pathological features into account, our data suggest that the DC3000 strain belongs toP. syringaepv. maculicola. This study shows thatP. syringaepv. maculicola andP. syringaepv. tomato appear multiclonal, as they did not diverge from a single common ancestral group within the ancestralP. syringaegenomospecies 3, and suggests that pathovar specificity withinP. syringaemay be due to independent genetic events.

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Section: Discussionmentioning

confidence: 99%

Housekeeping Gene Sequencing and Multilocus Variable-Number Tandem-Repeat Analysis To Identify Subpopulations within Pseudomonas syringae pv. maculicola and Pseudomonas syringae pv. tomato That Correlate with Host Specificity

Gironde

Manceau

2012

Appl Environ Microbiol

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“…VNTRs can be rapidly characterized by PCRs with specific primers based on the flanking regions of the tandem repeats. MLVA based on a few highly variable VNTRs usually displays a high level of discriminatory power in distinguishing closely related isolates for the investigation of disease outbreaks and epidemiological studies (16)(17)(18)(19)(20)(21). Vauterin et al (3) suggested the investigation of hrp (hypersensitive reaction and pathogenicity) genes to distinguish the nonpathogenic Xanthomonas strains from the pathogenic ones.…”

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confidence: 99%

“…Thus, highly discriminative typing methods are needed for surveillance and outbreak studies. Multilocus variable-number tandem-repeat (VNTR) analysis (MLVA) has been successfully developed for many bacterial species (16)(17)(18)(19)(20)(21). It is a bacterial typing method involving amplification and fragment size analysis of polymorphic regions of DNA containing variable numbers of tandem repeat sequences.…”

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confidence: 99%

Phylogenetic and Variable-Number Tandem-Repeat Analyses Identify Nonpathogenic Xanthomonas arboricola Lineages Lacking the Canonical Type III Secretion System

Essakhi

Cesbron

Saux

et al. 2015

Appl Environ Microbiol

118

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bXanthomonas arboricola is conventionally known as a taxon of plant-pathogenic bacteria that includes seven pathovars. This study showed that X. arboricola also encompasses nonpathogenic bacteria that cause no apparent disease symptoms on their hosts. The aim of this study was to assess the X. arboricola population structure associated with walnut, including nonpathogenic strains, in order to gain a better understanding of the role of nonpathogenic xanthomonads in walnut microbiota. A multilocus sequence analysis (MLSA) was performed on a collection of 100 X. arboricola strains, including 27 nonpathogenic strains isolated from walnut. Nonpathogenic strains grouped outside clusters defined by pathovars and formed separate genetic lineages. A multilocus variable-number tandem-repeat analysis (MLVA) conducted on a collection of X. arboricola strains isolated from walnut showed that nonpathogenic strains clustered separately from clonal complexes containing Xanthomonas arboricola pv. juglandis strains. Some nonpathogenic strains of X. arboricola did not contain the canonical type III secretion system (T3SS) and harbored only one to three type III effector (T3E) genes. In the nonpathogenic strains CFBP 7640 and CFBP 7653, neither T3SS genes nor any of the analyzed T3E genes were detected. This finding raises a question about the origin of nonpathogenic strains and the evolution of plant pathogenicity in X. arboricola. T3E genes that were not detected in any nonpathogenic isolates studied represent excellent candidates to be those responsible for pathogenicity in X. arboricola. E ubacteria constitute a major component of the commensal microbiota, and the nature of their interaction with plants is still unknown (1). Xanthomonas strains living in close association with plants but causing no apparent disease symptoms on their host have been reported (2, 3). On the basis of amplified fragment length polymorphism (AFLP) analysis, Gonzalez et al. (2) showed that nonpathogenic Xanthomonas strains colonizing cassava were clearly distinguished from Xanthomonas axonopodis pv. manihotis strains that cause cassava bacterial blight. Although some of these nonpathogenic strains have been characterized genetically and phenotypically, little is known about their epidemiological or ecological importance.In previous studies, the genetic diversity and population structure of Xanthomonas have been investigated using DNA-DNA hybridization (4, 5), repetitive-sequence PCR (rep-PCR) (4-7), AFLP (4, 8), and fluorescent AFLP (9, 10). As an alternative to these methods, the comparative sequence analysis of protein-encoding genes has also been widely explored. For example, Parkinson et al. (11) used the gyrB gene, which encodes the subunit B protein of DNA gyrase, for establishing a phylogenetic relationship among 203 Xanthomonas pathotype strains. Young et al. (12, 13) used a multilocus sequence analysis (MLSA) based on four genes (dnaK, encoding the chaperone protein; fyuA, encoding one tonB-dependent transporter; gyrB and rpoD, encoding the R...

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“…As S. Paratyphi A is known to be a highly clonal organism that pulsed-field gel electrophoresis (PFGE) or multilocus sequence typing (MLST) techniques can hardly discriminate, the NRC focused microbiological characterisation on antimicrobial susceptibility testing and attempted to discriminate isolates by using sequencing of the Clustered Regularly Interspaced Palindromic Repeats (CRISPR) contents, as previously described [7,8]. We carried out antimicrobial susceptibility testing on all available S. Paratyphi A isolates by using the disk diffusion method, with a panel of 32 antimicrobial drugs (Bio-Rad, Marnes-La-Coquette, France).…”

Section: Investigation Of the Event And Resultsmentioning

confidence: 99%

Unusual increase in reported cases of Paratyphoid A fever among travellers returning from Cambodia, January to September 2013

et al. 2013

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From January to September 2013, a marked increase in notifications of Salmonella Paratyphi A infections among travellers returning from Cambodia occurred in France. An investigation revealed 35 cases without a common source: 21 in France, five in Germany, three in the Netherlands, one in Norway, one in the United Kingdom, four in New-Zealand. Data suggest an ongoing event that should trigger further investigation. Travellers to Cambodia should observe preventive measures including good personal hygiene and food handling practices.

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Comparison of multilocus variable-number tandem repeat analysis and pulsed-field gel electrophoresis in molecular subtyping of Salmonella enterica serovars Paratyphi A

Cited by 20 publications

References 21 publications

Housekeeping Gene Sequencing and Multilocus Variable-Number Tandem-Repeat Analysis To Identify Subpopulations within Pseudomonas syringae pv. maculicola and Pseudomonas syringae pv. tomato That Correlate with Host Specificity

Housekeeping Gene Sequencing and Multilocus Variable-Number Tandem-Repeat Analysis To Identify Subpopulations within Pseudomonas syringae pv. maculicola and Pseudomonas syringae pv. tomato That Correlate with Host Specificity

Phylogenetic and Variable-Number Tandem-Repeat Analyses Identify Nonpathogenic Xanthomonas arboricola Lineages Lacking the Canonical Type III Secretion System

Unusual increase in reported cases of Paratyphoid A fever among travellers returning from Cambodia, January to September 2013

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