Comparison of motility and membrane integrity to assess rat sperm viability

Vetter, Christine; Miller, Judith E.; Crawford, Lisa M.; Armstrong, Michael J.; Clair, James St.; Conner, Michael W.; Wise, L. David; Skopek, Thomas R.

doi:10.1016/s0890-6238(97)00155-x

Cited by 28 publications

(22 citation statements)

References 28 publications

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“…Sperm abnormalities were present in the 1250-mg/kg dosage group on days 14 and 28 post treatment. In contrast to these ndings by Linder et al (1994aLinder et al ( , 1994b, Vetter et al (1998) identi ed a single DBA dosage of 1200 mg/kg/day to be a NOAEL for sperm abnormalities.…”

Section: Male Reproductive Effectsmentioning

confidence: 81%

Oral (Drinking Water) Two-Generation Reproductive Toxicity Study of Dibromoacetic Acid (DBA) in Rats

et al. 2002

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In a two-generation study of dibromoacetic acid (DBA), Crl SD rats (30 rats/sex/group/generation) were provided DBA in drinking water at 0 (reverse osmosis-deionized water), 50, 250, and 650 ppm (0, 4.4 to 11.6, 22.4 to 55.6, and 52.4 to 132.0 mg/kg/day, respectively; human intake approximates 0.1 microg/kg/day [0.0001 mg/kg/day]). Observations included viability, clinical signs, water and feed consumption, body and organ weights, histopathology, and reproductive parameters (mating, fertility, abortions, premature deliveries, durations of gestation, litter sizes, sex ratios and viabilities, maternal behaviors, reproductive organ weights, sperm parameters and implantation sites, sexual maturation). Histopathological evaluations were performed on at least 10 P and F1 rats/sex at 0 and 650 ppm (gross lesions, testes, intact epididymis; 10 F1 dams at 0, 250, and 650 ppm for primordial follicles). Developmental observations included implantations, pup numbers, sexes, viabilities, body weights, morphology, and reproductive performance. At 50 ppm and higher, both sexes and generations had increased absolute and relative liver and kidneys weights, and female rats in both generations had reduced absolute and relative adrenal weights; adrenal changes were probably associated with physiological changes in water balance. The livers and kidneys (10/sex/group/generation) had no histopathological changes. Other minimal effects at 50 ppm were reduced water consumption and a transient reduction in body weight. At 250 and 650 ppm, DBA reduced parental water consumption, body weight gains, body weights, feed consumption, and pup body weights. P and F1 generation male rats at 250 and 650 ppm had altered sperm production (retained step 19 spermatids in stages IX and X tubules sometimes associated with residual bodies) and some epididymal tubule changes (increased amounts of exfoliated spermatogenic cells/residual bodies in epididymal tubules, atrophy, and hypospermia), although inconsistently and at much lower incidences. Unilateral abnormalities of the epididymis (small or absent epididymis) at 650 ppm in four F1 generation male rats were considered reproductive tract malformations. The no-observable-adverse-effect level (NOAEL) and reproductive and developmental NOAELs for DBA were at least 50 ppm (4.5 to 11.6 mg/kg/day), 45,000 to 116,000 times the human adult exposure level. Reproductive and developmental effects did not occur in female rats exposed to DBA concentrations as high as 650 ppm. Based on the high multiples of human exposure required to produce effects in male rats, DBA should not be identified as a human reproductive or developmental risk.

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Section: Male Reproductive Effectsmentioning

confidence: 81%

Oral (Drinking Water) Two-Generation Reproductive Toxicity Study of Dibromoacetic Acid (DBA) in Rats

et al. 2002

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“…Plasma membrane integrity in culture medium with a normal K + and Na + ratio needs a supply of intracellular ATP. Vetter et al (1998) reported that membrane integrity is a necessary, although insufficient, criterion for predicting motility. In this study, a similar percentage number of sperm from transgenic or non-transgenic male rabbits stained for motility and SYBR-14 viability.…”

Section: Discussionmentioning

confidence: 99%

Viability and apoptosis in spermatozoa of transgenic rabbits

Chrenek

Makarevich

Simon

2010

Zygote

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The aim of our study was to compare the viability of sperm cells from transgenic (mWAP-hFVIII gene) or non-transgenic (normal) rabbit males as assessed by viability (SYBR-14/PI) and apoptosis (annexin V) tests. These results were evaluated using female conception rates following insemination with the respective sperm samples. No significant differences were found in concentration and motility between transgenic and non-transgenic spermatozoa. Spermatozoa from both transgenic (63.05 ± 20.05%) or non-transgenic (65.75 ± 22.15%) males, stained with SYBR-14 (green), were found to be morphologically normal. In both groups, the highest proportion of annexin V-positive sperm staining was found in the post-acrosomal part of the sperm head (8.66 and 27.53%). The percentage of sperm that stained with SYBR-14/PI or with annexin V/DAPI was correlated with liveborn in transgenic rabbits (R2 = 0.6118 and R2 = 0.2187, respectively) or non-transgenic rabbits (R2 = 0.671 and R2 = 0.3579, respectively). These data indicate that there was no difference in the viability of rabbit transgenic and non-transgenic spermatozoa when determined by both fluorescence assays.

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“…Sperm cell progressive movement is caused by the tail and is necessary to move towards the ovum for fertilization to take place (MORTIMER 1997). Sperm motility depends mainly on tail membrane properties (VETTER et al 1998). In our experiment, the best semen quality was observed in 8 to 10 week old males.…”

Section: Sexual Maturation In Common Vole Malesmentioning

confidence: 99%

Sexual Maturation in Common Vole (<I>Microtus arvalis</I>) Males Raised under Laboratory Conditions

Miska

Kruczek

Kapusta

2014

folia biol (krakow)

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MISKA A., KRUCZEK M., KAPUSTA J. 2014. Sexual maturation in Common vole (Microtus arvalis) males raised under laboratory conditions. Folia Biologica (Kraków) 62: 135-142.The common vole is one of the most numerous rodents in Europe and Asia but its reproductive biology is not fully described. It is thought that females reach reproductive abilities at a very young age, however, there is no data concerning male sexual maturation. The aim of the present study was to determine the rate of sexual maturation of males of the common vole. Research was carried out on 4, 6, 8 and 10 week-old animals. Body, testes and accessory sex glands weights were compared to evaluate morphological development. Epididymal sperm quality was assessed by a motility test, sperm tail membrane integrity, viability, maturity and sperm head morphology. Moreover, the number of sperm cells in 1 mm ! of semen was evaluated. The largest body weights were observed in 8 and 10 week-old males, which also possessed the highest relative weights of gonads and accessory sex glands. These groups of males produce about 2 times more gametes than 6 week-old individuals. The highest seamen quality was noted in 8 and 10 week-old males. Based on the obtained results, it is concluded that among the investigated age groups the most appropriate male age for fertile copulation is between 8 and 10 weeks.

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Comparison of motility and membrane integrity to assess rat sperm viability

Cited by 28 publications

References 28 publications

Oral (Drinking Water) Two-Generation Reproductive Toxicity Study of Dibromoacetic Acid (DBA) in Rats

Oral (Drinking Water) Two-Generation Reproductive Toxicity Study of Dibromoacetic Acid (DBA) in Rats

Viability and apoptosis in spermatozoa of transgenic rabbits

Sexual Maturation in Common Vole (<I>Microtus arvalis</I>) Males Raised under Laboratory Conditions

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