Comparison of Montanide adjuvants, IMS 3012 (Nanoparticle), ISA 206 and ISA 35 (Emulsion based) alongwith incomplete Freund's adjuvant for hyperimmunization of equines used for production of polyvalent snake antivenom

Waghmare, A.B.; Deopurkar, R. L.; Salvi, Nitin; Khadilkar, M. V.; Kalolikar, Milind; Gäde, S.

doi:10.1016/j.vaccine.2008.11.103

Cited by 41 publications

(18 citation statements)

References 22 publications

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“…Further studies are warranted to improve the manufacturing process to provide better yield, considering recent reports, describing a new non-mineral adjuvant that can minimize burden on the horses (24,25) and describing a stabilizer for freeze-drying of antivenom (26).…”

Section: Discussionmentioning

confidence: 99%

Venom and Antivenom of the Redback Spider (<i>Latrodectus hasseltii</i>) in Japan. Part II. Experimental Production of Equine Antivenom against the Redback Spider

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Venom and Antivenom of the Redback Spider (<i>Latrodectus hasseltii</i>) in Japan. Part II. Experimental Production of Equine Antivenom against the Redback Spider

et al. 2017

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“…The Montanide ISA series of adjuvants are ready-to-use W/O, O/W or W/O/W emulsions, incorporating high-grade, injectable mineral or non-mineral oils. Proven benefits in veterinary medicine include the production of stable vaccine emulsions with low viscosity, ease of injection, reduced toxicity, and induction of a strong, long-lasting immune response [6], [7], [23], [24], [25], [26], [27]. Montanide ISA adjuvants appear to be ideally suited for use with vaccines of limited immunogenicity, such as profilin, an intracellular component that contributes to actin-dependent gliding motility and host cell invasion of apicomplexan parasites, including Toxoplasma gondii and Eimeria spp.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Montanide™ ISA 71 VG Adjuvant during Profilin Vaccination against Experimental Coccidiosis

et al. 2013

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Chickens were immunized subcutaneously with an Eimeria recombinant profilin protein plus Montanide™ ISA 70 VG (ISA 70) or Montanide™ ISA 71 VG (ISA 71) water-in-oil adjuvants, or with profilin alone, and comparative RNA microarray hybridizations were performed to ascertain global transcriptome changes induced by profilin/ISA 70 vs. profilin alone and by profilin/ISA 71 vs. profilin alone. While immunization with profilin/ISA 70 vs. profilin alone altered the levels of more total transcripts compared with profilin/ISA 71 vs. profilin alone (509 vs. 296), the latter was associated with a greater number of unique biological functions, and a larger number of genes within these functions, compared with the former. Further, canonical pathway analysis identified 10 pathways that were associated with genes encoding the altered transcripts in animals immunized with profilin/ISA 71 vs. profilin alone, compared with only 2 pathways in profilin/ISA 70 vs. profilin alone. Therefore, ISA 71 was selected as a candidate adjuvant in conjunction with profilin vaccination for in vivo disease protection studies. Vaccination with profilin/ISA 71 was associated with greater body weight gain following E. acervulina infection, and decreased parasite fecal shedding after E. maxima infection, compared with profilin alone. Anti-profilin antibody levels were higher in sera of E. maxima- and E. tenella-infected chickens vaccinated with profilin/ISA 71 compared with profilin alone. Finally, the levels of transcripts encoding interferon-γ, interleukin (IL)-2, IL-10, and IL-17A were increased in intestinal lymphocytes from E. acervulina-, E. maxima-, and/or E. tenella-infected chickens vaccinated with profilin/ISA 71 compared with profilin alone. None of these effects were seen in chickens injected with ISA 71 alone indicating that the adjuvant was not conferring non-specific immune stimulation. These results suggest that profilin plus ISA 71 augments protective immunity against selective Eimeria species in chickens.

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“…cobras or Naja naja , krait or Bungarus caeruleus , Russell's viper or Daboia russelli , and saw-scaled viper or Echis carinatus . [5] World health organization (WHO) has recognized snakebite as neglected and important public health problem in rural areas of tropical and subtropical countries situated in Asia, Africa, Oceania, and Latin America. [6] According to the same WHO report, the global annual incidence of envenoming and resulting deaths ranges from a minimum of 421,000 to a maximum of 1,841,000 and 20,000 to 94,000, respectively.…”

Section: Introductionmentioning

confidence: 99%

Clinical profile, species-specific severity grading, and outcome determinants of snake envenomation: An Indian tertiary care hospital-based prospective study

Saravu

Somavarapu

Shastry

et al. 2012

Indian Journal of Critical Care Medicine

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Objective:We undertook this study to assess the clinical profile and outcome determinants of different snake envenomation as well as to assign species-specific severity grade to different cases based on clinico – laboratory evidence scale.Materials and Methods:A prospective clinico – epidemiologic evaluation for outcome determinants of snakebite envenomation was carried out based on a clinico – laboratory severity grading scale, among 76 patients over a period of 2 years, in a tertiary care hospital in southern India.Results:Majority of patients were male agricultural workers (53.9%) followed by housewives (19.7%), and students (9.2%). Occurrence of viper snake envenomation with hemotoxic syndrome (73.68%) was highest followed by cobra and krait envenomation with neurotoxic (19.73%) and hemo – neurotoxic (5.3%) syndrome, respectively. On the contrary, maximum mortality and severity was seen in krait (60%) followed by cobra (13.33%) and viper (8.9%) envenomation. The average dose of anti-snake venom (ASV) administered varied from 9.83 (±7.22) to 20.25 (±4.92) vials throughout grade I to IV in all snake species envenomation. An increase in severity grade, ASV dose, and mortality were observed with the corresponding delay in ‘bite to needle time.’ Also, initial traditional treatments and krait species envenomation were significantly associated with higher grades of severity and mortality.Conclusion:There is an urgent need to spread awareness among the community for avoidance of traditional treatment and any delay in medical intervention in snakebite incidents.

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Comparison of Montanide adjuvants, IMS 3012 (Nanoparticle), ISA 206 and ISA 35 (Emulsion based) alongwith incomplete Freund's adjuvant for hyperimmunization of equines used for production of polyvalent snake antivenom

Cited by 41 publications

References 22 publications

Venom and Antivenom of the Redback Spider (<i>Latrodectus hasseltii</i>) in Japan. Part II. Experimental Production of Equine Antivenom against the Redback Spider

Venom and Antivenom of the Redback Spider (<i>Latrodectus hasseltii</i>) in Japan. Part II. Experimental Production of Equine Antivenom against the Redback Spider

Evaluation of Montanide™ ISA 71 VG Adjuvant during Profilin Vaccination against Experimental Coccidiosis

Clinical profile, species-specific severity grading, and outcome determinants of snake envenomation: An Indian tertiary care hospital-based prospective study

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