Evaluation of Montanide™ ISA 71 VG Adjuvant during Profilin Vaccination against Experimental Coccidiosis

Abstract

Chickens were immunized subcutaneously with an Eimeria recombinant profilin protein plus Montanide™ ISA 70 VG (ISA 70) or Montanide™ ISA 71 VG (ISA 71) water-in-oil adjuvants, or with profilin alone, and comparative RNA microarray hybridizations were performed to ascertain global transcriptome changes induced by profilin/ISA 70 vs. profilin alone and by profilin/ISA 71 vs. profilin alone. While immunization with profilin/ISA 70 vs. profilin alone altered the levels of more total transcripts compared with profilin/ISA 71 vs. profilin alone (509 vs. 296), the latter was associated with a greater number of unique biological functions, and a larger number of genes within these functions, compared with the former. Further, canonical pathway analysis identified 10 pathways that were associated with genes encoding the altered transcripts in animals immunized with profilin/ISA 71 vs. profilin alone, compared with only 2 pathways in profilin/ISA 70 vs. profilin alone. Therefore, ISA 71 was selected as a candidate adjuvant in conjunction with profilin vaccination for in vivo disease protection studies. Vaccination with profilin/ISA 71 was associated with greater body weight gain following E. acervulina infection, and decreased parasite fecal shedding after E. maxima infection, compared with profilin alone. Anti-profilin antibody levels were higher in sera of E. maxima- and E. tenella-infected chickens vaccinated with profilin/ISA 71 compared with profilin alone. Finally, the levels of transcripts encoding interferon-γ, interleukin (IL)-2, IL-10, and IL-17A were increased in intestinal lymphocytes from E. acervulina-, E. maxima-, and/or E. tenella-infected chickens vaccinated with profilin/ISA 71 compared with profilin alone. None of these effects were seen in chickens injected with ISA 71 alone indicating that the adjuvant was not conferring non-specific immune stimulation. These results suggest that profilin plus ISA 71 augments protective immunity against selective Eimeria species in chickens.

Evaluation of vaccine delivery systems for inducing long-lived antibody responses to Dermanyssus gallinae antigen in laying hens

“…Montanide™ ISA 71 VG has been used previously to immunize hens with Eimeria recombinant profilin protein because of the enhanced immune repertoire generated by this adjuvant (Jang et al, 2013). In the current study, this method of antigen administration proved to be the most superior for inducing antigenspecific serum IgY.…”

Protection against infectious bronchitis virus by spike ectodomain subunit vaccine

“…Furthermore, a peptide near the amino terminal end of S2 has been shown to induce a protective cell-mediated response [37]. The adjuvant used has been reported to stimulate both antibody and cell-mediated immune responses [38][39][40]. The addition of the HA2 domain of the influenza hemagglutinin has also been demonstrated to increase the immunogenicity and protective capacity of IBV S1, possibly by increasing thermostability [41].…”

Montanide ISA 71 VG is Advantageous to Freund's Adjuvant in Immunization Against S. aureus Infection of Mice

“…As Jang et al . reported in their vaccination studies with poultry infected with different Eimeria strains, ISA 71 VG adjuvant combined with their antigen of interest provided superior humoral and cellular immune responses. Recent observations with IFN‐ γ ‐ and IL‐17‐deficient mice lead to the notion that the stimulation of a cellular response seems necessary to generate a protective vaccine against S. aureus .…”

“… have demonstrated that adjuvants direct isotype‐switching processes via inducing a certain cytokine pattern. FA and the Montanide ™ are known to increase the production of IFN‐ γ via Th1 stimulation, leading to a subclass switching towards IgG2a and should therefore be equally potent in inducing a protective immune response against gram‐positive bacteria .…”