Comparison of modified Chicago sky blue stain and potassium hydroxide mount for the diagnosis of dermatomycoses and onychomycoses

Zhong, Linda L. D.; Sheng, Ping; Yang, Yanping; Wen, Li; Huang, Wenming; Wang, Jie-Di; Fan, Yi‐Ming

doi:10.1016/j.mimet.2015.02.013

Cited by 10 publications

(12 citation statements)

References 5 publications

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“…[9] Instantaneous results in less than 5 min can be obtained by a slight modification of the staining technique by flame heating the CSB stained slide. [6]…”

Section: Discussionmentioning

confidence: 99%

“…Molecular methods such as polymerase chain reaction are expensive and not available for general use. [56]…”

Section: Introductionmentioning

confidence: 99%

“…Chicago Sky Blue (CSB) is a novel contrast stain that highlights the fungal hyphae blue in color against a purplish background of cellular debris and it was found to be more sensitive than KOH mount in diagnosis of pityriasis versicolor and dermatomycoses. [5678] There is a paucity of Indian studies on the utility of this stain in fungal infections. Here, we compared the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of CSB stain and KOH mount in the diagnosis of dermatophytoses.…”

Section: Introductionmentioning

confidence: 99%

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A novel contrast stain for the rapid diagnosis of dermatophytoses: A cross-sectional comparative study of chicago sky blue 6b stain, potassium hydroxide mount and culture

Baddireddy

Poojary

2019

Indian J Dermatol

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Background: The current upsurge of complicated dermatophytosis in India and other parts of the world has necessitated the development of rapid and accurate diagnostic techniques. Conventional methods such as potassium hydroxide (KOH) mount lack color contrast and require a trained eye, whereas fungal culture is laborious and costly. Chicago sky blue 6B (CSB) a new contrast stain promises to be a reliable and rapid diagnostic method. It contains 1% CSB stain and is used with 10% KOH as clearing agent. Aims and Objectives: The objective of this study is to compare the efficacy of a novel contrast stain (CSB) and KOH mount in the diagnosis of dermatophytoses with culture as the reference method. Methods: The infected skin scrapings, nail clippings, and hair specimens from 100 patients, with clinically diagnosed dermatophytoses, were subjected to KOH mount, CSB stain, and culture on Sabouraud's dextrose agar. Using fungal culture as the reference standard, sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of CSB stain and KOH mount were determined. An inter-rater reliability analysis was performed using the Cohen's κ statistic to determine agreement amongst the different diagnostic modalities. Results: Of the 100 cases, the CSB stain and KOH mount were positive in 85 (85%) and 70 (70%) cases, respectively. Cultures on Sabouraud's agar yielded growth in 59 (59%) cases. The sensitivity, specificity, PPV, NPV, and accuracy of CSB stain were 98%, 34%, 68%, 93%, and 72%, respectively. The same for KOH mount were 71%, 32%, 60%, 43%, and 55%, respectively. Conclusion: CSB stain is a simple, rapid, sensitive, accurate, and inexpensive office-based method with qualitatively superior demonstration of dermatophytes compared to KOH mount.

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“…[9] Instantaneous results in less than 5 min can be obtained by a slight modification of the staining technique by flame heating the CSB stained slide. [6]…”

Section: Discussionmentioning

confidence: 99%

“…Molecular methods such as polymerase chain reaction are expensive and not available for general use. [56]…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel contrast stain for the rapid diagnosis of dermatophytoses: A cross-sectional comparative study of chicago sky blue 6b stain, potassium hydroxide mount and culture

Baddireddy

Poojary

2019

Indian J Dermatol

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“…Diagnosis of superficial mycoses can be confirmed by a modified Chicago sky blue stain and potassium hydroxide mount. 5 Bromhidrosis is characterised by sweat that has an offensive odour. Rather than follicular involvement, excessive secretions from either apocrine or eccrine glands become malodorous on bacterial breakdown.…”

Section: Answermentioning

confidence: 99%

Doctor, my armpit hair has turned yellow! Malodorous yellowish axillary hair in an adolescent patient

2018

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“…The sensitivity of CSB stain is superior than KOH. 7 There is various of diagnostic result from some previous study so we conduct the diagnostic test of sensitivity and specificity of CSB compared to ink Parker TM blue-black KOH in dermatophytoses patients. In this study using CSB solution consist of KOH 10% + Chicago Sky Blue 6B 1%.…”

Section: Introductionmentioning

confidence: 99%

A comparative study of Chicago Sky Blue and ParkerTM ink blue black potassium hidroxide in the diagnosis of dermatophytes

Sukmawati¹,

Rahmadewi²,

Ervianti³

2019

Dermatol Reports

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Parker ink blue black potassium hydroxide (KOH) is a regular stain for the diagnosis of dermatophytoses. KOH giving less color contrast to the fungal element. The Chicago Sky Blue (CSB) solution is a new contrast have various sensitivity and specificity values. This is analytical observational,cross sectional design study conducted at Dermatomycology Division of Outpatient Clinic, Dr. Soetomo General Hospital. 40 samples from lesion and perilesional infected area, diagnosed dermatophytoses with culture positive was include in this study. All two tests were performed examined by KOH and CSB. The slides were examined after incubation in x 10 and x 40 magnifications by experienced observer. The sensitivity, specificity and McNemar test of CSB and KOH was calculated using culture as the standard test. The sensitivities, specificities, PPV, NPV of the Chicago sky blue and KOH respectively are 95% and 100%, 95,24% and 100%, 100% and 100%, with McNemar test p = 1 and Kappa score is 0,950. The Chicago Sky Blue stain provides a good color contrast and IT could be a alternative staining for the dermatophytoses.

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Comparison of modified Chicago sky blue stain and potassium hydroxide mount for the diagnosis of dermatomycoses and onychomycoses

Cited by 10 publications

References 5 publications

A novel contrast stain for the rapid diagnosis of dermatophytoses: A cross-sectional comparative study of chicago sky blue 6b stain, potassium hydroxide mount and culture

A novel contrast stain for the rapid diagnosis of dermatophytoses: A cross-sectional comparative study of chicago sky blue 6b stain, potassium hydroxide mount and culture

Doctor, my armpit hair has turned yellow! Malodorous yellowish axillary hair in an adolescent patient

A comparative study of Chicago Sky Blue and ParkerTM ink blue black potassium hidroxide in the diagnosis of dermatophytes

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