Comparison of methods for the detection ofSalmonella in poultry

Langkabel, Nina; Klose, Alexander D.; Irsigler, Herlinde; Jaeger, Dorothea; Bräutigam, L.; Hafez, H. M.; Fries, R.

doi:10.3382/japr.2013-00885

Cited by 6 publications

(2 citation statements)

References 16 publications

(13 reference statements)

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“…Accurate assessment of the impact of in-feed preharvest interventions on the reduction of foodborne pathogens such as SE is complex and challenging due to a number of experimental and commercial considerations, particularly challenge dose and organism, administration or exposure routes (e.g., direct gavage, seeding models, and environmental seeding), production system type, sample type (e.g., feces, cloacal swab, ceca, and litter), animal age, temporal considerations, and laboratory analytical methodology (4,7,8,12,15,18,29,31,44). Challenge dose can influence colonization, concentrations, and transmission within various experimental models and subsequently the ability to detect and quantify Salmonella at various points in time from various sample types (7,17).…”

Section: Discussionmentioning

confidence: 99%

Effect of Feeding a Postbiotic Derived from Saccharomyces cerevisiae Fermentation as a Preharvest Food Safety Hurdle for Reducing Salmonella Enteritidis in the Ceca of Layer Pullets

Gingerich

Frana

Smith

et al. 2021

Journal of Food Protection

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Salmonella Enteritidis is responsible for a significant proportion of foodborne Salmonellosis in the United States and continues to be attributable to table eggs, despite increased federal oversight in recent years. Technologies, including feed additives, continue to be evaluated for pre-harvest application of their potential food safety benefits. Here, Diamond V Original XPC™, a Saccharomyces cerevisiae fermentation based postbiotic (SCFP), was evaluated for its effectiveness in the reduction of Salmonella Enteritidis (SE) colonization in young layer pullets. A total of forty, day-old Hy-Line W-36 layer pullets were equally divided and randomly assigned to one of two dietary treatments with SCFP, or without (PCON) and orally gavaged on Day 28 with 10 6 CFU/mL of SE. Another twenty, day-old layer pullets were fed the same control feed without SCFP and blank inoculated on Day 28 with 1 ml of sterile PBS to serve as a negative control (NCON). Qualitative and quantitative analyses of cecal Salmonella contents were performed for all birds on Day 32. The prevalence of SE in the ceca of all directly challenged birds was 100%, however, the SE concentration in birds fed SCFP diet (3.35 Log 10 CFU/g) was significantly lower ( P <0.0001) than that of the PCON birds not fed SCFP (4.49 Log 10 CFU/g). Moreover, the proportion of SE colonized individuals with enumerable SE concentrations was lower in SCFP fed birds (57.9%) when compared to the PCON (95.0%). These data suggest that inclusion of SCFP in the diet may aid in the reduction of SE within the ceca of commercial laying hens and could serve as an additional, pre-harvest food safety hurdle.

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Section: Discussionmentioning

confidence: 99%

Effect of Feeding a Postbiotic Derived from Saccharomyces cerevisiae Fermentation as a Preharvest Food Safety Hurdle for Reducing Salmonella Enteritidis in the Ceca of Layer Pullets

Gingerich

Frana

Smith

et al. 2021

Journal of Food Protection

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“…The sampling of hens occurs monthly or during outbreaks. However, it is well documented that cloacal swabs are not direct indicators of colonization ( Langkabel et al, 2014 ; Battersby et al, 2017 ). Therefore, by using a “spot check” strategy that looks directly at the egg and the GIT of embryonic chicks is valuable.…”

Section: Discussionmentioning

confidence: 99%

Semi-Quantification of Total Campylobacter and Salmonella During Egg Incubations Using a Combination of 16S rDNA and Specific Pathogen Primers for qPCR

et al. 2018

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Rapid molecular techniques that evaluate eggs for the presence of foodborne pathogens is an essential component to poultry food safety monitoring. Interestingly, it is not just table eggs that contribute to outbreaks of foodborne disease. Broiler layer production actively contributes to sustaining of foodborne pathogens within a flock. The surface contamination of production eggs with invasive pathogens such as Salmonella enterica, Campylobacter jejuni, and Listeria monocytogenes during embryogenesis results in gastrointestinal tract (GIT) colonization. Pathogens that secure a niche within the GIT during embryonic development are nearly impossible to eradicate from the food chain. Therefore, current monitoring paradigms are not comprehensive because they fail to capture the presence of invasive pathogens within the embryonic GIT rapidly. By developing tools to recognize the pathogens’ presence in the GIT during embryogenesis, producers are then able to spot evaluate broiler eggs for their potential risk as carriers of foodborne pathogens. In this study a novel qPCR assay was developed to semi-quantify pathogen load relative to total bacterial burden. Eggs sampled from three independent production broiler flocks of different ages were assayed for S. enterica (invA), C. jejuni (HipO), and L. monocytogenes (HlyA) against total microbial load (16s). The eggs were sampled at 1-day post-set within each flock, 2 weeks post-set, after vaccination (at 2.5 weeks) and 1-day post-hatch. The eggs were washed, and the yolk and embryonic chick GIT were collected. The DNA was extracted and subjected to a qPCR assay. The results confirm a novel technique for pathogen monitoring relative to total bacterial load and a unique method for monitoring the dynamics of foodborne pathogen invasion throughout broiler egg production.

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Prevalence and related factors of Salmonella spp. and Salmonella Typhimurium contamination among broiler farms in Kerman province, Iran

Firouzabadi

Saadati

Najimi

et al. 2020

Preventive Veterinary Medicine

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Comparison of methods for the detection ofSalmonella in poultry

Cited by 6 publications

References 16 publications

Effect of Feeding a Postbiotic Derived from Saccharomyces cerevisiae Fermentation as a Preharvest Food Safety Hurdle for Reducing Salmonella Enteritidis in the Ceca of Layer Pullets

Effect of Feeding a Postbiotic Derived from Saccharomyces cerevisiae Fermentation as a Preharvest Food Safety Hurdle for Reducing Salmonella Enteritidis in the Ceca of Layer Pullets

Semi-Quantification of Total Campylobacter and Salmonella During Egg Incubations Using a Combination of 16S rDNA and Specific Pathogen Primers for qPCR

Prevalence and related factors of Salmonella spp. and Salmonella Typhimurium contamination among broiler farms in Kerman province, Iran

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