Comparison of metalloproteinase protein and activity profiling

Giricz, Orsi; Lauer, J.; Fields, Gregg B.

doi:10.1016/j.ab.2010.09.040

Cited by 10 publications

(7 citation statements)

References 68 publications

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“…In vitro studies on skin cancer revealed that most melanoma cell lines, but not normal melanocytes, express MMP8 mRNA [57]. Yet at the protein level, MMP8 was only present in half of the nine melanoma cell lines examined by Giricz et al [115]. Similarly, MMP8 mRNA expression in keratinocytes of varying tumorigenic potential was detected in vitro but protein expression varied [116].…”

Section: Resultsmentioning

confidence: 99%

The Role of MMP8 in Cancer: A Systematic Review

Juurikka

Butler

Salo

et al. 2019

IJMS

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Matrix metalloproteinases (MMPs) have traditionally been considered as tumor promoting enzymes as they degrade extracellular matrix components, thus increasing the invasion of cancer cells. It has become evident, however, that MMPs can also cleave and alter the function of various non-matrix bioactive molecules, leading to both tumor promoting and suppressive effects. We applied systematic review guidelines to study MMP8 in cancer including the use of MMP8 as a prognostic factor or as a target/anti-target in cancer treatment, and its molecular mechanisms. A total of 171 articles met the inclusion criteria. The collective evidence reveals that in breast, skin and oral tongue cancer, MMP8 inhibits cancer cell invasion and proliferation, and protects patients from metastasis via cleavage of non-structural substrates. Conversely, in liver and gastric cancers, high levels of MMP8 worsen the prognosis. Expression and genetic alterations of MMP8 can be used as a prognostic factor by examination of the tumor and serum/plasma. We conclude, that MMP8 has differing effects on cancers depending on their tissue of origin. The use of MMP8 as a prognostic factor alone, or with other factors, seems to have potential. The molecular mechanisms of MMP8 in cancer further emphasize its role as an important regulator of bioactive molecules.

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Section: Resultsmentioning

confidence: 99%

The Role of MMP8 in Cancer: A Systematic Review

Juurikka

Butler

Salo

et al. 2019

IJMS

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“…MMP‐3 activity was found to be significantly higher in synovial fluid from patients suffering from rheumatoid arthritis (RA) compared with control subjects. Our laboratory recently described an in situ MMP cell‐based assay with FRET peptide substrates in which no transfer of reagents is needed 19. MMP activity assays with three different FRET substrates were utilized to determine whether protein production correlated with function for melanoma WM‐266‐4 and fibroblast BJ cell lines.…”

Section: Introductionmentioning

confidence: 99%

Chemical Biology for Understanding Matrix Metalloproteinase Function

Knapinska¹,

Fields²

2012

ChemBioChem

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The matrix metalloproteinase (MMP) family has long been associated with normal physiological processes such as embryonic implantation, tissue remodeling, organ development, and wound healing, as well as multiple aspects of cancer initiation and progression, osteoarthritis, inflammatory and vascular diseases, and neurodegenerative diseases. The development of chemically designed MMP probes has advanced our understanding of the roles of MMPs in disease in addition to shedding considerable light on the mechanisms of MMP action. The first generation of protease-activated agents has demonstrated proof of principle as well as providing impetus for in vivo applications. One common problem has been a lack of agent stability at nontargeted tissues and organs due to activation by multiple proteases. The present review considers how chemical biology has impacted the progress made in understanding the roles of MMPs in disease and the basic mechanisms of MMP action.

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“…Secreted MMPs are produced in inactive forms and must be activated extracellularly to perform their functions, which is then regulated by protease inhibitors. Therefore, activity assays may be more representative of the role of MMPs in pathogenic states than protein production analysis 48 . Thus, we quantitatively measured MMP activity using zymography in the current study.…”

Section: Discussionmentioning

confidence: 99%

Differential expression of aqueous humor microRNAs in central retinal vein occlusion and its association with matrix metalloproteinases: a pilot study

Hong

Hwang

et al. 2022

Sci Rep

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The aim of this study is to investigate the differential expression of microRNAs (miRNAs) in the aqueous humor (AH) of patients with central retinal vein occlusion (CRVO), and their association with AH matrix metalloproteinase (MMP) activity. Eighteen subjects, including 10 treatment naïve patients with CRVO and 8 control subjects, scheduled for intravitreal injection and cataract surgery, respectively, were included. AH samples were collected at the beginning of the procedure. A microarray composed of 84 miRNAs was performed to identify differentially expressed miRNAs in CRVO AH, which were further analyzed using bioinformatic tools to identify directly related cytokines/proteins. Eight miRNAs (hsa-mir-16-5p, hsa-mir-142-3p, hsa-mir-19a-3p, hsa-mir-144-3p, hsa-mir-195-5p, hsa-mir-17-5p, hsa-mir-93-5p, and hsa-mir-20a-5p) were significantly downregulated in the CRVO group. Bioinformatic analysis revealed a direct relationship among downregulated miRNAs, CRVO, and the following proteins: MMP-2, MMP-9, tumor necrosis factor, transforming growth factor beta-1, caspase-3, interleukin-6, interferon gamma, and interleukin-1-beta. Activities of MMP-2 and -9 in AH were detected using gelatin zymography, showing significant increase in the CRVO group compared to the control group (p < 0.01). This pilot study first revealed that MMP-2 and -9 were directly related to downregulated miRNAs and showed significant increase in activity in AH of patients with CRVO. Therefore, the relevant miRNAs and MMPs in AH could serve as potential biomarkers or therapeutic targets for CRVO.

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Comparison of metalloproteinase protein and activity profiling

Cited by 10 publications

References 68 publications

The Role of MMP8 in Cancer: A Systematic Review

The Role of MMP8 in Cancer: A Systematic Review

Chemical Biology for Understanding Matrix Metalloproteinase Function

Differential expression of aqueous humor microRNAs in central retinal vein occlusion and its association with matrix metalloproteinases: a pilot study

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