Chemical Biology for Understanding Matrix Metalloproteinase Function

Knapinska, Anna M.; Fields, Gregg B.

doi:10.1002/cbic.201200298

Cited by 50 publications

(45 citation statements)

References 132 publications

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“…The bacterial collagen was stabilized by charge clusters (as Pro was not modified to Hyp), and as presented herein, charge clustering can also modulate MMP activity. MMP substrates have been developed for in vivo analysis of MMP activity (77,78). One of the key considerations for these probes is the selectivity of the substrate (77,78).…”

Section: Nomentioning

confidence: 99%

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The Role of Collagen Charge Clusters in the Modulation of Matrix Metalloproteinase Activity

Lauer

Bhowmick

Tokmina‐Roszyk

et al. 2014

Journal of Biological Chemistry

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Background:The role of charged clusters in modulating MMP hydrolysis of collagen is unknown. Results: Triple-helical peptides containing charged motifs were as good or better substrates than the parent (non-chargeclustered) peptide. Conclusion: MMP-2 and MMP-9 greatly favored the presence of charged residues. Significance: The lack of Gly-Asp-Lys clusters in native collagens may diminish potential MMP-2 and MMP-9 collagenolytic activity.

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Section: Nomentioning

confidence: 99%

“…MMP substrates have been developed for in vivo analysis of MMP activity (77,78). One of the key considerations for these probes is the selectivity of the substrate (77,78). Our group has previously described a THP fluorescence resonance energy transfer substrate that is selective for MMP-2, MMP-9, and MMP-12 (58,79).…”

Section: Nomentioning

confidence: 99%

The Role of Collagen Charge Clusters in the Modulation of Matrix Metalloproteinase Activity

Lauer

Bhowmick

Tokmina‐Roszyk

et al. 2014

Journal of Biological Chemistry

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“…Recent work has revealed the important role of NF-κB in macrophage migration [20] . Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of the extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis, intracerebral hemorrhage, and metastasis [18,21] . Symptomatological and histological evidence from recent work has shown that the activity of MMP-9 increases in the unstable carotid plaques in atherogenesis [22] .…”

Section: Introductionmentioning

confidence: 99%

Exendin-4 ameliorates oxidized-LDL-induced inhibition of macrophage migration in vitro via the NF-κB pathway

Chen

Yin

et al. 2013

Acta Pharmacol Sin

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Aim: To investigate the effects of the glucagon-like peptide-1 (GLP-1) receptor agonist exendin-4 on oxidized low-density lipoprotein (ox-LDL)-induced inhibition of macrophage migration and the mechanisms underlying the effects of exendin-4. Methods: Primary peritoneal macrophages were extracted from the peritoneal cavity of mice treated with 3% thioglycollate (2 mL, ip). Migration of the macrophages was examined using a cell migration assay. Macrophage migration-related factors including leptin-like ox-LDL receptor (LOX-1), cyclooxygenase 2 (COX-2), tumor necrosis factor (TNF)-α, interleukin-1 (IL-1)β, matrix metalloproteinase-2 (MMP-2), intercellular adhesion molecule (ICAM)-1 and macrophage migration inhibitory factor (MIF) were measured using semiquantitative RT-PCR. Expression of MIF and ICAM-1 proteins was examined with ELISA. Gelatin zymography was used to evaluate the activity of MMP-9. Activation of the NF-κB pathway was determined by confocal laser scanning microscopy. Results: Treatment of the macrophages with ox-LDL (50 μg/mL) markedly suppressed the macrophage migration. Furthermore, ox-LDL treatment substantially increased the expression of the macrophage migration-related factors, the activity of MMP-9 and the translocation of the NF-κB p65 subunit. These effects of ox-LDL were significantly ameliorated by pretreatment with the specific NF-κB inhibitor ammonium pyrrolidine dithiocarbamate (100 μmol/L). These effects of ox-LDL were also significantly ameliorated by pretreatment with exendin-4 (25 and 50 nmol/L). Conclusion: Exendin-4 ameliorates the inhibition of ox-LDL on macrophage migration in vitro, via suppressing ox-LDL-induced expression of ICAM-1 and MIF, which is probably mediated by the NF-κB pathway.

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“…Matrix metalloproteases are a family of extracellular zinc-dependent endopeptidases that are capable of destroying all types of extracellular matrix proteins [35]. At present, about 30 enzymes of this family are isolated.…”

Section: Discussionmentioning

confidence: 99%

Ontogenetic Approach to the Study of Mechanisms of Copper-Induced Liver Fibrosis

Божков¹,

Klimova²,

Nikitchenko³

et al. 2017

AAR

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On the model of Cu-induced liver fibrosis, the relationship between the activity of prooxidant-antioxidant system, immune system parameters, liver morphology and several physiological parameters (body temperature and performance ability of the animals, taking into account their ages) was investigated. Classical biochemical, immunological, histological and physiological methods of investigation were used. The subjects of the study were male Wistar rats of 3-month (young) and 20-month (old) age. For the induction of liver fibrosis, experimental animals were successively injected with copper sulfate (three times at intervals of 24 hours at a dose corresponding to 33% of the lethal one). It was shown that after five days of sulfate copper administration inflammatory reactions in the liver, damage of the vessel epithelium, an increase in collagen content, and other morphological changes were detected. At this time, the content of lipid hydroperoxides in the liver and blood serum was increased, the activity of a number of antioxidant enzymes was reduced, and the activity of aconitase was two times less compared to values in the control group. These changes correlated with a decrease in the amount and activity of phagocytic cells in the blood of experimental animals. Inhibition of the general metabolism was accompanied by a decrease in body temperature, loss of body weight and performance ability. The relationship between a specific metabolic pattern in animals with Cu-induced fibrosis was age-dependent. The formed specific adaptive metabolic pattern is unstable, and in the future it can be realized in one of three possible adaptive strategies, the choice of which is influenced by age.

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Chemical Biology for Understanding Matrix Metalloproteinase Function

Cited by 50 publications

References 132 publications

The Role of Collagen Charge Clusters in the Modulation of Matrix Metalloproteinase Activity

The Role of Collagen Charge Clusters in the Modulation of Matrix Metalloproteinase Activity

Exendin-4 ameliorates oxidized-LDL-induced inhibition of macrophage migration in vitro via the NF-κB pathway

Ontogenetic Approach to the Study of Mechanisms of Copper-Induced Liver Fibrosis

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