Comparison of metabolic effects of EGF, TGF-α, and TGF-β1 in primary culture of fetal bovine myoblasts and rat L6 myoblasts

Chemotaxis of skeletal muscle satellite cells

“…One possibility is that the anti-mitotic effects of TGF-b are diminished at higher concentrations, as has been demonstrated for bovine satellite cells (Blachowski et al, 1993). Alternatively, because the response of myogenic cells to TGF-b depends upon the presence of other peptide growth factors (Allen and Boxhorn, 1989;Zentella and Massagú e, 1992;Blachowski et al, 1993;Cook et al, 1993), the migrating cells may encounter a pro-mitotic environment as they approach the site of injury. According to this model (Fig.…”

“…Both rat and pig satellite cells exhibit 50-60% inhibition of proliferation at concentrations of TGF-b in the 0.1-0.5 ng/ml range (Allen and Boxhorn, 1987;Pampusch et al, 1990;Cook et al, 1993). Bovine satellite cells are highly sensitive to TGF-b; maximum inhibition occurs at 0.001 ng/ml, and mitotic inhibition diminishes at higher concentrations (Blachowski et al, 1993). In these studies, the cells were already in the cell cycle after stimulation by serum mitogens, but TGF-b is also able to prevent the G 0 = G 1 transition of quiescent satellite cells.…”

TGF-alpha, EGF, and their cognate EGF receptor are co-expressed with desmin during embryonic, fetal, and neonatal myogenesis in mouse tongue development

“…Several studies indicate that growth factors are involved at successive stages of the myogenic cell lineage program (Lim and Hauschka, 1984;Smith et al, 1989;Rosa et al, 1988;Paterno et al, 1989). EGF and TGFa have been found to be potent mitogens for gastric smooth muscles (Yuan et al, 1993), whereas TGFa significantly inhibits DNA synthesis in primary cultures of fetal bovine skeletal muscle cells and rat L6 myoblasts (Blachowski et al, 1993). Transgenic mice over-expressing TGFa under the control of the metallothionein promoter resulted in a significant reduction in the size and weight of skeletal muscle (Luetteke et al, 1993a).…”

Relationships between Pork Quality Traits and Growth Factor Concentrations in Serum and Longissimus dorsi Muscle before and at Slaughter in Female Market Pigs

“…In contrast to IGFs, transforming-growth factor-β (TGF-β) family peptides suppress myogenesis (McCroskery et al, 2003;White, 2008 Kollias andMcDermott, 2008). Epidermal growth factor (EGF), which stimulates proliferation and protein synthesis of myogenic cells usually in synergy with IGF in vitro (Blachowski et al, 1993Mau et al, 2008, is known to suppress somatic growth in vivo (Chernausek et al, 1991;Chan and Wong, 2000;Xian, 2007). Besides these, several other regulatory peptides including fibroblast growth factor and platelet-derived growth factor are known to be involved in regulation of proliferation and differentiation of myogenic cell lineages (Florini et al, 1991;Doumit et al, 1993).…”