Comparison of manganese oxide nanoparticles and manganese sulfate with regard to oxidative stress, uptake and apoptosis in alveolar epithelial cells

Frick, R.; Müller‐Edenborn, Björn; Schlicker, A.; Rothen‐Rutishauser, Barbara; Raemy, David; Günther, Detlef; Hattendorf, Bodo; Stark, Wendelin J.; Beck‐Schimmer, Beatrice

doi:10.1016/j.toxlet.2011.05.1037

Cited by 63 publications

(59 citation statements)

References 50 publications

(55 reference statements)

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“…The production of reactive oxygen species (ROS) by BFO HNPs in cell cultures was assessed using two fluorescence assays: dihydroethidium (DHE) and carboxydichlorodihydrofluorescein diacetate (DCFH-DA). 16,17 We could detect a dose-dependent increase of ROS, more pronounced in A549 cells than in HTB-182 ( Fig. 2C1 and C2), which remains however low compared to that induced by other metal-based NPs.…”

mentioning

confidence: 80%

Deep UV generation and direct DNA photo-interaction by harmonic nanoparticles in labelled samples

et al. 2014

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A novel bio-photonics approach based on the nonlinear optical process of second harmonic generation by non-centrosymmetric nanoparticles is presented and demonstrated on malignant human cell lines. The proposed method allows to directly interact with DNA in absence of photosensitizing molecules, to enable independent imaging and therapeutic modalities switching between the two modes of operation by simply tuning the excitation laser wavelength, and to avoid any risk of spontaneous activation by any natural or artificial light source.

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mentioning

confidence: 80%

Deep UV generation and direct DNA photo-interaction by harmonic nanoparticles in labelled samples

et al. 2014

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“…The composition of nanoparticle-containing medium solutions was characterized by inductively coupled plasma optical emission spectroscopy (ICP OES, Horiba Ultra 2). A detailed description of the nanoparticle preparation is given elsewhere 11 . More detailed information is enclosed in the supporting information.…”

Section: Methodsmentioning

confidence: 99%

“…We demonstrated that the effects in MAC are trolox-sensitive, but only can speculate that ROS-induced modified activity of several protein kinases regulating inflammatory pathways might cause the measured changes in MCP-1 expression 41 . It has certainly to be mentioned that the influence of antioxidants already present in cells and epithelial lining fluid (gluthatione, ascrobate, uric acid) might play an important role in the modification of the inflammatory response 11 . In addition, MAC and AEC were observed under in vitro conditions using rat cell lines, which do not accurately mimic the conditions in a living organism.…”

Section: Ros-mediated Decrease In Viability Of Macrophages Only Margmentioning

confidence: 99%

“…The particles are comparable regarding morphology, size, shape, and degree of agglomeration, but differ in redox activity and intracellular solubility 10 . The particle concentration in the experiments was set within the range of 1 to 6 µg per cm 2 cell surface (corresponds to 5 to 20 mg * kg -1 cell culture media), in analogy to previous published data with these particles 11 . The chosen amounts of particles may simulate an exposure scenario during particle synthesis 12 .…”

Section: Introductionmentioning

confidence: 99%

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Inflammatory Response of Lung Macrophages and Epithelial Cells after Exposure to Redox Active Nanoparticles: Effect of Solubility and Antioxidant Treatment

Urner

Schlicker

Z’graggen

et al. 2014

Environ. Sci. Technol.

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The effects of an exposure to three mass-produced metal oxide nanoparticles-similar in size and specific surface area but different in redox activity and solubility-were studied in rat alveolar macrophages (MAC) and epithelial cells (AEC). We hypothesized that the cell response depends on the particle redox activity and solubility determining the amount of reactive oxygen species formation (ROS) and subsequent inflammatory response. MAC and AEC were exposed to different amounts of Mn3O4 (soluble, redox-active), CeO2 (insoluble, redox-active), and TiO2 (insoluble, redox-inert) up to 24 h. Viability and inflammatory response were monitored with and without coincubation of a free-radical scavenger (trolox). In MAC elevated ROS levels, decreased metabolic activity and attenuated inflammatory mediator secretion were observed in response to Mn3O4. Addition of trolox partially resolved these changes. In AEC, decreased metabolic activity and an attenuated inflammatory mediator secretion were found in response to CeO2 exposure without increased production of ROS, thus not sensitive to trolox administration. Interestingly, highly redox-active soluble particles did not provoke an inflammatory response. The data reveal that target and effector cells of the lung react in different ways to particle exposure making a prediction of the response depending on redox activity and intracellular solubility difficult. ABSTRACTThe effects of an exposure to three mass-produced metal oxide nanoparticles -similar in size and specific surface area, but different in redox activity and solubility -were studied in rat alveolar macrophages (MAC) and epithelial cells (AEC). We hypothesized that the cell response depends on the particle redox activity and solubility determining the amount of reactive oxygen species formation (ROS) and subsequent inflammatory response. MAC and AEC were exposed to different amounts of Mn 3 O 4 (soluble, redox-active), CeO 2 (insoluble, redox-active), and TiO 2 (insoluble, redox-inert) up to 24 hours. Viability and inflammatory response were monitored with and without co-incubation of a free-radical scavenger (trolox).In MAC elevated ROS levels, decreased metabolic activity and attenuated inflammatory mediator secretion were observed in response to Mn 3 O 4 . Addition of trolox partially resolved these changes. In AEC, decreased metabolic activity and an attenuated inflammatory mediator secretion were found in response to CeO 2 exposure without increased production of ROS, thus not sensitive to trolox administration. Interestingly, highly redox-active soluble particles did not provoke an inflammatory response. The data reveal that target and effector cells of the lung react in different ways to particle exposure making a prediction of the response depending from redox activity and intracellular solubility difficult.

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“…[48] Within cells, ceria nanoparticles tend to agglomerate. [48,49] Cytotoxic effects of nanoceria can be greatly reduced by preventing the internalization of the NPs into cells, for example by encapsulating them into a hydrogel. Weaver and Stabler [50] demonstrated that their alginate hydrogel prevented the phagocytosis of dextran-coated nanoceria and thus reduced their cytotoxicity, while preserving the antioxidative and protective properties of these particles.…”

Section: Toxicity Of Nanoceriamentioning

confidence: 99%

Toxicity and Protective Effects of Cerium Oxide Nanoparticles (Nanoceria) Depending on Their Preparation Method, Particle Size, Cell Type, and Exposure Route

Gagnon

Fromm

2015

Eur J Inorg Chem

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Nanoceria (cerium oxide nanoparticles) toxicity is currently a concern because of its use in motor vehicles in order to reduce carbon monoxide (CO), nitrogen oxides (NOx), and hydrocarbons in exhaust gases. In addition, many questions arise with respect to its biomedical applications exploiting its potential to protect cells against irradiation and oxidative stress. Indeed, toxicology studies on nanoceria report results that seem contradictory, demonstrating toxic effects in some studies, protective effects in others, and sometimes little or no effect at all. The variability in the experimental setups and particle characterization makes these studies difficult to compare and the toxicity of newly developed nanoceria materials challenging to predict. This microreview aims to compare the toxicity of nanoceria in terms of preparation method, particle size, concentration, host organism, and exposure method.

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Comparison of manganese oxide nanoparticles and manganese sulfate with regard to oxidative stress, uptake and apoptosis in alveolar epithelial cells

Cited by 63 publications

References 50 publications

Deep UV generation and direct DNA photo-interaction by harmonic nanoparticles in labelled samples

Deep UV generation and direct DNA photo-interaction by harmonic nanoparticles in labelled samples

Inflammatory Response of Lung Macrophages and Epithelial Cells after Exposure to Redox Active Nanoparticles: Effect of Solubility and Antioxidant Treatment

Toxicity and Protective Effects of Cerium Oxide Nanoparticles (Nanoceria) Depending on Their Preparation Method, Particle Size, Cell Type, and Exposure Route

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