Comparison of liquid chromatography, capillary electrophoresis and super-critical fluid chromatography in the determination of Losartan Potassium drug substance in Cozaar® tablets

Williams, Reed C.; Alasandro, Mark; Fasone, V.L.; Boucher, Robert; Edwards, J. F.

doi:10.1016/0731-7085(96)01740-2

Cited by 70 publications

(17 citation statements)

References 11 publications

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“…The use of water as a solvent in chemical analysis laboratories has many advantages in terms of environmental aspects, cost and safety compared with the use of an organic solvent. Therefore, considering the losartan potassium aqueous solubility of 3.3 mg mL 1 (Lastra et al, 2003;Williams et al, 1996) and the factors mentioned above, the use of distilled water as solvent was deemed adequate for our validation purposes, making the methods simple and convenient.…”

Section: Resultsmentioning

confidence: 99%

“…The literature has described several analytical methods for losartan potassium determination in tablets, when used as a single active principle or in combination with hydrochlorothiazide, using high performance liquid chromatography (HPLC), capillary electrophoresis, super critical fluid chromatography, high performance thin layer chromatography and derivative ultraviolet spectrophotom etry (Ansari et al, 2004; Erk, 2001;Lastra et al, 2003;Mccarthy et al, 1998;Williams et al, 1996). For losartan potassium determination in pharmaceutical capsules, two HPLC methods have been reported (Bonfilio et al, 2009;Maio, Dias, Bergold, 2005).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…However, its adverse effects are different, mainly regarding coughing, whose incidence is significantly lower compared with ACE inhibitors (Oigman, 1996). Losartan potassium is a light yellow solid with a mo lecular weight of 461; melting point of 183.5 184.5 ºC; aqueous solubility of 3.3 mg mL 1 at pH 7.8 and pKa value of 4.9 (Lastra et al, 2003;Williams et al, 1996).The literature has described several analytical methods for losartan potassium determination in tablets, when used as a single active principle or in combination with hydrochlorothiazide, using high performance liquid chromatography (HPLC), capillary electrophoresis, super critical fluid chromatography, high performance thin layer chromatography and derivative ultraviolet spectrophotom etry (Ansari et al, 2004; Erk, 2001;Lastra et al, 2003;Mccarthy et al, 1998;Williams et al, 1996). For losartan potassium determination in pharmaceutical capsules, two HPLC methods have been reported (Bonfilio et al, 2009;Maio, Dias, Bergold, 2005).…”

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Comparative study of analytical methods by direct and first-derivative UV spectrophotometry for evaluation of losartan potassium in capsules

Bonfílio

Favoretto

Pereira

et al. 2010

Braz. J. Pharm. Sci.

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Losartan potassium is an antihypertensive non peptide agent, which exerts its action by specific blockade of angiotensin II receptors. The aim of the present study was the validation and application of analytical methods for the quality control of losartan potassium 50 mg in pharmaceutical capsules, using direct and first derivative UV spectrophotometry. Based on losartan potassium spectrophotometric characteristics, a signal at 205 nm of the zero order spectrum and a signal at 234 nm of the first derivative spectrum, were found adequate for quantification. The results were used to compare these instrumental techniques. The linearity between the signals and concentrations of losartan potassium in the ranges of 3.0 7.0 mg L 1 and 6.0 14.0 mg L 1 for direct and first derivative spectrophotometry in aqueous solutions, respectively, presented a correlation coefficient (r) of 0.9999 in both cases. The methods were applied for losartan potassium in capsule dosage obtained from local pharmacies, and were shown to be efficient, easy to apply and low cost. These methods do not use polluting reagents and require relatively inexpensive equipment.Uniterms: Losartan potassium. Direct UV spectrophotometry. First derivative UV spectrophotometry.O losartano potássico é um agente anti hipertensivo não peptídico, que exerce sua ação por bloqueio específico dos receptores da angiotensina II. Este trabalho propôs a validação e aplicação de métodos analíticos orientados ao controle de qualidade de losartano potássico 50 mg na forma farmacêutica cápsula, utilizando a espectrofotometria direta e derivada de primeira ordem na região do UV. Baseado nas características espectrofotométricas de losartano potássico, um sinal a 205 nm do espectro de ordem zero e um sinal a 234 nm do espectro de primeira derivada foram adequados para a quantificação. Os resultados foram usados para comparar essas duas técnicas instrumentais. O coeficiente de correlação entre as respostas e as concentrações de losartano potássico na faixa de 3,0 7,0 mg L 1 e 6,0 14,0 mg L 1 para espectrofotometria direta e derivada de primeira ordem em solução aquosa, respectivamente, foi de (r) of 0,9999 para ambos os casos. Os métodos foram aplicados para quantificação de losartano potássico em cápsulas obtidas de farmácias de manipulação locais e demonstraram ser eficientes, fáceis de aplicar e de baixo custo. Além disso, não necessitam de reagentes poluentes e requerem equipamentos economicamente viáveis.Unitermos: Losartano potássico. Espectrofotometria direta. Espectrofotometria derivada de primeira ordem. INTRODUCTIONLosartan potassium (Figure 1) is an imidazole derivative, chemically described as 2 butyl 4 chloro 1 [p-(o-1H-tetrazol 5 ylphenyl)benzyl]imidazole 5 methanol monopotassium salt. Losartan was developed by DuPont Merck laboratories as a potent non peptide angiotensin II receptor (type AT 1 ) antagonist for hyper tension treatment (Barreiro, Fraga, 2001;Korolkovas, 2007). Losartan is administered in its active form and R. Bonfilio, L. B. Favoretto, G. R. Perei...

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Comparative study of analytical methods by direct and first-derivative UV spectrophotometry for evaluation of losartan potassium in capsules

Bonfílio

Favoretto

Pereira

et al. 2010

Braz. J. Pharm. Sci.

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“…However, several methods have been described for the determination of Losartan potassium drug substance in tablets. Various methods developed are HPLC [21] [22], spectrophotometric [23] [24], capillary electrophoresis (CE) [25]- [28], HPTLC [29] [30] voltamatric [31] and liquid chromatography electrospray ionization tandem mass spectrometry [32].…”

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UPLCMS Method Development and Validation of Amlodipine, Hydrochlorthiazide and Losartan in Combined Tablet Dosage Form

Tengli¹,

Shivakumar²,

Gurupadayya³

2015

AJAC

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A simple, rapid, sensitive and specific UPLCMS method was developed and validated following ICH guidelines for simultaneous estimation of tablet dosage form containing amlodipine (AMLO) hydrochlorothiazide (HCT) and losartan (LOSAT) using telmisartan (TELMI) as an internal standard (IS). The separation was achieved using Waters ACQUITY BEH C18 (1.7 µm, 2.1 × 50 mm) column with gradient mode, mobile phase containing acetonitrile (A) & 1% ammonium acetate (B) pH adjusted to 2.8 with trifluoro acetic acid with gradient mode. The flow rate was 0.4 mL•mL −1 and the injection volume 2 µl. The retention time for amlodipine, hydrochlorothiazide and losartan was found to be 3.7, 2.5 and 3.9 min, respectively. The developed method was found to be linear over the concentration range of 50 -300 ng•mL −1 , 125 -750 ng•mL −1 and 500 -3000 ng•mL −1 for AMLO, HCT and LOSAT respectively. The signal intensities obtained in ion mode for amlodipine, hydrochlorothiazide, losartan and telmisartan (IS) they were found to be much higher positive ion mode (M+)− parent ion at m/z, 409.02, 297.97, 422.91 and 515.03, respectively, in QUATTROZQ full scan mass spectra.

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Application of capillary zone electrophoresis to the screening of some angiotensin II receptor antagonists

González¹,

Akesolo²,

Jiménez³

et al. 2002

Electrophoresis

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A capillary zone electrophoretic (CZE) method was optimized for the separation of five angiotensin II receptor antagonists (Losartan, Irbesartan, Valsartan, Telmisartan and Eprosartan) and two of their metabolites (EXP 3174 and Candesartan M1) by means of experimental design methodologies. The aim of this study was to define rapidly experimental conditions under which the analytes can be resolved for quantitation. The effects of the buffer (pH, concentration and composition), the organic modifier and voltage were studied. Critical factors were identified in a screening design (fractional factorial design) and sequentially an optimization design (central composite design) was used to choose optimal conditions for separation. The most favorable electrophoretic conditions were found by setting the resolution at a threshold value (Rs < or = 1.5) and minimizing, if possible, analysis time. Successful results were obtained with a 50 mM potassium dihydrogen phosphate:boric acid (25:75 v/v) buffer at pH 5.5 in the presence of 5% methanol and application of a 25 kV voltage. Analysis time was 8 min in a conventional fused-silica capillary (50 cm effective length) in a normal cationic mode (anode at the inlet and cathode at the outlet) after hydrostatical sample injection for 30 s.

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Comparison of liquid chromatography, capillary electrophoresis and super-critical fluid chromatography in the determination of Losartan Potassium drug substance in Cozaar® tablets

Cited by 70 publications

References 11 publications

Comparative study of analytical methods by direct and first-derivative UV spectrophotometry for evaluation of losartan potassium in capsules

Comparative study of analytical methods by direct and first-derivative UV spectrophotometry for evaluation of losartan potassium in capsules

UPLCMS Method Development and Validation of Amlodipine, Hydrochlorthiazide and Losartan in Combined Tablet Dosage Form

Application of capillary zone electrophoresis to the screening of some angiotensin II receptor antagonists

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