Application of capillary zone electrophoresis to the screening of some angiotensin II receptor antagonists

Abstract

A capillary zone electrophoretic (CZE) method was optimized for the separation of five angiotensin II receptor antagonists (Losartan, Irbesartan, Valsartan, Telmisartan and Eprosartan) and two of their metabolites (EXP 3174 and Candesartan M1) by means of experimental design methodologies. The aim of this study was to define rapidly experimental conditions under which the analytes can be resolved for quantitation. The effects of the buffer (pH, concentration and composition), the organic modifier and voltage were studied. Critical factors were identified in a screening design (fractional factorial design) and sequentially an optimization design (central composite design) was used to choose optimal conditions for separation. The most favorable electrophoretic conditions were found by setting the resolution at a threshold value (Rs < or = 1.5) and minimizing, if possible, analysis time. Successful results were obtained with a 50 mM potassium dihydrogen phosphate:boric acid (25:75 v/v) buffer at pH 5.5 in the presence of 5% methanol and application of a 25 kV voltage. Analysis time was 8 min in a conventional fused-silica capillary (50 cm effective length) in a normal cationic mode (anode at the inlet and cathode at the outlet) after hydrostatical sample injection for 30 s.

Losartan

“…González et al [48] reported a capillary zone electrophoretic (CZE) method for the separation of five AIIRAs (losartan, irbesartan, valsartan, telmisartan, and eprosartan) and two of their metabolites (EXP3174 and Candesartan M1) by means of experimental design methodologies. The aim of this study was to define rapidly experimental conditions under which the analytes can be resolved for quantitation.…”

Development and Validation of HPLC Method for Simultaneous Determination of Amlodipine, Valsartan, Hydrochlorothiazide in Dosage Form and Spiked Human Plasma

“…However, there is very few analytical methods reported for the simultaneous analysis of these drugs in a combined dosage formulation [3,4]. The reported methods are UV spectrophotometric [5][6][7][8][9], Spectrofluorimetric [10] HPLC [11][12][13][14][15][16][17][18][19][20][21][22][23][24], HPTLC [25,26], capillary electrophoresis [27][28][29][30] and electrochemical [31][32][33][34] methods.…”

Rapid determination of telmisartan in pharmaceuticals and serum by the parallel catalytic hydrogen wave method

“…TE yielded a stable and well-defined reduction wave in supporting electrolytes such as KH 2 PO 4 -Na 2 HPO 4 , Na 2 B 4 O 7 -KH 2 PO 4 , and NH 3 .H 2 O-NH 4 Cl buffers. However, the best-defined reduction peak was obtained in NH 3 .H 2 O-NH 4 Cl buffer. Therefore, this work selected the NH 3 .H 2 O-NH 4 Cl buffer as supporting electrolyte.…”

“…Among analytical techniques for the determination of TE, capillary zone electrophoretic (CZE) methods [2,3,4] and a micellar electrophoretic (MEKC) method [5] have been described. However, these methods need expensive equipment, are time-consuming, and require rigid experimental conditions, such as the amount of reagent, reaction temperature, and reaction time.…”