Comparison of lipopolysaccharide structures of Bordetella pertussis clinical isolates from pre- and post-vaccine era

Albitar-Nehme, Sami; Basheer, Soorej M.; Njamkepo, Elisabeth; Brisson, Jean‐Robert; Guiso, Nicole; Caroff, Martine

doi:10.1016/j.carres.2013.05.002

Cited by 23 publications

(15 citation statements)

References 45 publications

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“…The B. holmesii lipid A structures established in the present study differ from one to the other:Presence of free amino GlcN on the phosphate groups of lipid A structure: in Bho1 as in ATCC 51541, the failure of BvgA to function leads to a weaker substitution than in the two other isolates.Presence of 10:0(3-OH) at C-3, which we already observed in other Bordetella species such as B. pertussi s [31], is thought to be the consequence of the lack of activity of the 3 O -deacylase PagL, even if genomic explanation could not be found to support this hypothesis.The 14:0(2-OH) and 12:0(2-OH) fatty acids found in all the presented structures were previously shown to be important structural features for the infection of human macrophages [42]. …”

Section: Discussioncontrasting

confidence: 45%

“…Moreover, other two small peaks ( m / z 1925 and 1869) in FR4020 and FR4101 lipid A mass spectra gave the same mass difference with m / z 1764 and 1708, suggesting that these lipid A molecular species were carrying 2 GlcN, one at each phosphate groups. This modification is described here for the fifth time in lipid A species of the Bordetella genus [27,31,32,33,34]. Analogous to the FR4020 mass spectrum with the exception of peaks related to GlcN addition at m / z 1538, 1869 and 1925 (Figure 2) the mass spectrum for lipid A from Bho1 displayed the same peaks as FR4020 mass spectrum.…”

Section: Resultsmentioning

confidence: 60%

“…The two clinical isolates FR4020 and FR4101 displayed a higher degree of modification compared to Bho1 and the ATCC strain. Such an hexosamine modification has been described in other Bordetellae species such as B. pertussis , B. bronchiseptica and B. avium and was attributed to a lgm locus encoding a glycosyl transferase ArnT [31,35,39]. An orthologous sequence corresponding to lgm locus was identified in the genome of the three clinical isolates as in the ATCC 51541 reference-strain genome with no sequence difference between them.…”

Section: Discussionmentioning

confidence: 63%

“…Presence of 10:0(3-OH) at C-3, which we already observed in other Bordetella species such as B. pertussi s [31], is thought to be the consequence of the lack of activity of the 3 O -deacylase PagL, even if genomic explanation could not be found to support this hypothesis.…”

Section: Discussionmentioning

confidence: 68%

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Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541

Bouchez

Albitar-Nehme

Новиков

et al. 2017

IJMS

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Bordetella holmesii can cause invasive infections but can also be isolated from the respiratory tract of patients with whooping-cough like symptoms. For the first time, we describe the lipid A structure of B. holmesii reference strain ATCC 51541 (alias NCTC12912 or CIP104394) and those of three French B. holmesii clinical isolates originating from blood (Bho1) or from respiratory samples (FR4020 and FR4101). They were investigated using chemical analyses, gas chromatography–mass spectrometry (GC–MS), and matrix-assisted laser desorption ionization–mass spectrometry (MALDI–MS). The analyses revealed a common bisphosphorylated β-(1→6)-linked d-glucosamine disaccharide with hydroxytetradecanoic acid in amide linkages. Similar to B. avium, B. hinzii and B. trematum lipids A, the hydroxytetradecanoic acid at the C-2′ position are carrying in secondary linkage a 2-hydroxytetradecanoic acid residue resulting of post-traductional biosynthesis modifications. The three clinical isolates displayed characteristic structural traits compared to the ATCC 51541 reference strain: the lipid A phosphate groups are more or less modified with glucosamine in the isolates and reference strain, but the presence of 10:0(3-OH) is only observed in the isolates. This trait was only described in B. pertussis and B. parapertussis strains, as well as in B. petrii isolates by the past. The genetic bases for most of the key structural elements of lipid A were analyzed and supported the structural data.

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Section: Discussioncontrasting

confidence: 45%

Section: Resultsmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 68%

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Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541

Bouchez

Albitar-Nehme

Новиков

et al. 2017

IJMS

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“…Although lacking the O antigen, B. pertussis' LOS displays immunomodulatory and antigenic activities and remains pyrogenic, toxic and can activate and induce tumor necrosis factor production in macrophages [40]. Recent studies have also demonstrated that this endotoxin is stable at the structural level in B. pertussis clinical isolates [37]. Toxins are however not sufficient on their own to promote a successful colonization and B. pertussis also relies on the production of molecular anchors named adhesins.…”

Section: The B Pertussis Los Endotoxinmentioning

confidence: 99%

Bordetella pertussisand pertactin-deficient clinical isolates: lessons for pertussis vaccines

Hégerlé

Guiso²

2014

Expert Review of Vaccines

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Bordetella pertussis causes whooping cough in humans, a highly transmissible respiratory disease life threatening for unvaccinated infants. Vaccination strategies were thus introduced worldwide with great success in developed countries reaching high vaccine coverage with efficacious vaccines. In the late 20th/early 21st century, acellular pertussis vaccines replaced whole cell pertussis vaccines but B. pertussis still circulates and evolves in humans, its only known reservoir. The latest transformation of this pathogen, and of its close relative Bordetella parapertussis, is the loss of pertactin production, a virulence factor included in different acellular pertussis vaccines. The real impact of this evolution on acellular pertussis vaccines efficacy and effectiveness should be assessed through standardized surveillance and isolation of B. pertussis and B. parapertussis worldwide.

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Chemical Synthesis and Immunological Evaluation of a Pentasaccharide Bearing Multiple Rare Sugars as a Potential Anti‐pertussis Vaccine

et al. 2020

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With the infection rate of Bordetella pertussis at a 60‐year high, there is an urgent need for new anti‐pertussis vaccines. The lipopolysaccharide (LPS) of B. pertussis is an attractive antigen for vaccine development. With the presence of multiple rare sugars and unusual glycosyl linkages, the B. pertussis LPS is a highly challenging synthetic target. In this work, aided by molecular dynamics simulation and modeling, a pertussis‐LPS‐like pentasaccharide was chemically synthesized for the first time. The pentasaccharide was conjugated with a powerful carrier, bacteriophage Qβ, as a vaccine candidate. Immunization of mice with the conjugate induced robust anti‐glycan IgG responses with IgG titers reaching several million enzyme‐linked immunosorbent assay (ELISA) units. The antibodies generated were long lasting and boostable and could recognize multiple clinical strains of B. pertussis, highlighting the potential of Qβ‐glycan as a new anti‐pertussis vaccine.

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Comparison of lipopolysaccharide structures of Bordetella pertussis clinical isolates from pre- and post-vaccine era

Cited by 23 publications

References 45 publications

Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541

Bordetella holmesii: Lipid A Structures and Corresponding Genomic Sequences Comparison in Three Clinical Isolates and the Reference Strain ATCC 51541

Bordetella pertussisand pertactin-deficient clinical isolates: lessons for pertussis vaccines

Chemical Synthesis and Immunological Evaluation of a Pentasaccharide Bearing Multiple Rare Sugars as a Potential Anti‐pertussis Vaccine

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