Comparison of lipid composition ofAedes aegypti andAedes albopictus cells obtained from logarithmic and stationary phases of growth

Abstract

The total lipids, total neutral lipids, and total phospholipids from Aedes aegypti and aedes albopictus cells cultivated in vitro in a medium containing fetal calf serum were analyzed. The mosquito cells were harvested in the logarithmic and stationary phases of growth. The fatty acid profiles of the lipids showed differences during the aging of the cells but not betweeen species. There was an increase in chain elongation and unsaturation of the fatty acids in the stationary phase when compared with the logarithmic phase of growth. The major components of the fatty acid profiles of the cells were 16:0, 16:1, and 18:1 fatty acids. Few similarities were found between the lipid analysis of the mosquito cells and the growth medium.

Neutral lipid composition ofCulex quinquefasciatus andCulex tritaeniorhynchus cells at two phases of growth

“…Methylation of the classes of neutral lipids, extraction of the esters, and fatty acid analysis by gas liquid chromatography followed the procedures previously reported (4).…”

“…All reagents were purified as described by Makino et al (14). Reference materials were obtained from sources previously listed (4). Gravimetric analyses were performed as previously described (14).…”

“…Mosquito cells, Aedes aegypti species, cultivated in vitro usually maintained an arbovirus infection without cell lysis in contrast to infected mammalian cells (2). Lipids extracted from two Aedes species of larval cells grown in a cell culture medium were examined for metabolic changes and/or a lipid-virus interaction (3,4).…”

Comparison of the lipid composition ofCulex quinquefasciatus andCulex tritaeniorhynchus cells obtained from the logarithmic and stationary phases of growth

“…The lipids of Diptera differ from those of mammalian cells in fatty acid profiles and phospholipid composition, and thus, with infection, changes in lipids between the two cells might be noted. However, changes have been observed in lipid composition of insects at different stages of metamorphosis (14) and of insect cells grown in cultures with aging (15). It would be necessary to determine the lipid composition of the cells grown in tissue culture at a particular phase of development and to observe changes that occurred during development before studying the effect of arbovirus infection on lipid content of the cells.…”

“…A. aegypti and A. albopictus larvae cells have been grown in spinner culture (15,16). The fatty acid profiles of the total lipid, total neutral lipids, and total phospholipids were determined at logarithmic and stationary phases of growth.…”