Comparison of lawsone contents among Lawsonia inermis plant parts and neurite outgrowth accelerators from branches

Oda, Yoshimi; Nakashima, Souichi; Kondo, Erina; Nakamura, Seikou; Yano, Mamiko; Kubota, Chisa; Masumoto, Yusuke; Hirao, Minami; Ogawa, Yuki; Matsuda, Hisashi

doi:10.1007/s11418-018-1221-y

Cited by 24 publications

(16 citation statements)

References 21 publications

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“…inermis contained 2-hydroxy-1,4-naphthoquinone (lawsone). HPLC analysis showed that the extracts of Lawsonia inermis flowers, leaves and branches contained 116.9, 486.2 and 5.4 μg/g lawsone [31].…”

Section: Lawsoniamentioning

confidence: 99%

A Review on Lawsonia Inermis: A Potential Medicinal Plant

Al-Snafi

2019

Int J Curr Pharm Sci

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Lawsonia inermis (Family: Lythraceae) contained carbohydrates, phenolic, flavonoids, saponins, proteins, alkaloids, terpenoids, quinones, coumarins, xanthones, fat, resin and tannins. It also contained 2-hydroxy-1,4-naphthoquinone (lawsone). Many alkaloids, naphthoquinone derivatives, phenolics and flavonoids were isolated from different parts of Lawsonia inermis. The pharmacological studies showed that Lawsonia inermis showed antibacterial, antifungal, antiparasitic, molluscicidal, antioxidant, hepatoprotective, central nervous, analgesic, anti-inflammatory, antipyretic, wound and burn healing, immunomodulatory, antiurolithiatic, antidiabetic, hypolipidemic, antiulcer, antidiarrhoeal, diuretic, anticancer and many other pharmacological effects. The current review will highlight the chemical constituents and pharmacological effects of Lawsonia inermis.Lawsonia inermis Linn (Family: Lythraceae) which is commonly known as henna, mainly present in subtropical and tropical areas and is used in all over the world. It was used for over 9000 y for its cosmetic values as a dye. The phytochemical analysis of Lawsonia inermis revealed the presence of carbohydrates, phenolic, flavanoids, saponins, proteins, alkaloids, terpenoids, quinones, coumarins, xanthones, fat, resin and tannins. It also contained 2-hydroxy-1,4naphthoquinone (lawsone). Many alkaloids, napthoquinone derivatives, phenolics and flavonoids were isolated from different parts of Lawsonia inermis. The pharmacological studies showed that Lawsonia inermis showed antibacterial, antifungal, antiparasitic, molluscicidal, antioxidant, hepatoprotective, central nervous, analgesic, anti-inflammatory, antipyretic, wound and burn healing, immunomodulatory, antiurolithiatic, antidiabetic, hypolipidemic, antiulcer, antidiarrhoeal, diuretic, anticancer and many other pharmacological effects. The current review will highlight the chemical constituents and pharmacological effects of Lawsonia inermis. Plant profile SynonymsAlcanna spinosa, Leaves of Lawsonia inermis provide an important cosmetic dye. Henna leaves were extensively used for centuries in the Middle East, the Far East and Northern Africa as dye for nails, hands, hair and textile. Henna was also used in treating skin problems, headache, jaundice, amebiasis and enlargement of the spleen [20,21]. Parts used medicinallyWhole plant, roots, fruits, stem, leaves, barks, inflorescence, rhizome, bulbs, latex, seeds, flowers and oil were used in different ailments [20]. Physiochemical characteristicsPhysiochemical investigation of leaf showed that the total ash was (14.60 %), acid insoluble ash (4.50 %), water soluble ash (3.0 %), loss on drying (4.5 %), alcohol soluble extractive value (3.8 % w/w) and aqueous extractive value (5.0 % w/w) [22]. Chemical constituentsThe preliminary phytochemical analysis of the aqueous extract of Lawsonia inermis revealed the presence of carbohydrates, phenolic compounds, flavonoids, saponins, proteins, alkaloids, terpenoids, 2 quinones, coumarins, xanthones, 6% fat, 2-3% resin and 7-8%...

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Section: Lawsoniamentioning

confidence: 99%

A Review on Lawsonia Inermis: A Potential Medicinal Plant

Al-Snafi

2019

Int J Curr Pharm Sci

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“…Lawsone is a characteristic compound in the leaves and flowers of Lawsonia inermis L. [25]. An intermediate, 1,4-naphthoquinone can be found, for example, in black walnut leaves.…”

Section: Introductionmentioning

confidence: 99%

Effects of Naphthazarin (DHNQ) Combined with Lawsone (NQ-2-OH) or 1,4-Naphthoquinone (NQ) on the Auxin-Induced Growth of Zea mays L. Coleoptile Segments

Rudnicka

Ludynia

Karcz

2019

IJMS

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Naphthoquinones, plants secondary metabolites are known for their antibacterial, antifungal, anti-inflammatory, anti-cancer and anti-parasitic properties. The biological activity of naphthoquinones is connected with their ability to generate reactive oxygen species and to modify biological molecules at their nucleophilic sites. In our research, the effect of naphthazarin (DHNQ) combined with 2-hydroxy-1,4-naphthoquinone (NQ-2-OH) or 1,4-naphthoquinone (1,4-NQ) on the elongation growth, pH changes of the incubation medium, oxidative stress and redox activity of maize coleoptile cells were investigated. This paper describes experiments performed with maize (Zea mays L.) coleoptile segments, which is a classical model system to study plant cell elongation growth. The data presented clearly demonstrate that lawsone and 1,4-naphthoquinone combined with naphthazarin, at low concentrations (1 and 10 nM), reduced the endogenous and IAA-induced (Indole-3-Acetic Acid) elongation growth of maize coleoptile segments. Those changes in growth correlated with the proton concentration in the incubation medium, which suggests that the changes in the growth of maize coleoptile segments observed in the presence of naphthoquinones are mediated through the activity of PM H+-ATPase. The presence of naphthoquinones induced oxidative stress in the maize coleoptile tissue by producing hydrogen peroxide and causing changes in the redox activity. Moreover, the incubation of maize segments with both naphthoquinones combined with naphthazarin resulted in lipid peroxidation and membrane damage. The regulation of PM H+-ATPase activity, especially its inhibition, may result from two major types of reaction: first, a direct interaction between an enzyme and naphthoquinone, which leads to the covalent modification of the protein thiols and the generation of thioethers, which have been found to alter the activity of the PM H+-ATPases; second, naphthoquinones induce reactive oxygen species (ROS) production, which inhibits PM H+-ATPases by increasing cytosolic Ca2+. This harmful effect was stronger when naphthazarin and 1,4-naphthoquinone were added together. Taking these results into account, it can be suggested that by combining naphthoquinones in small quantities, an alternative to synthetic pesticides could be developed.

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“…Therefore, we expected that L. inermis flowers and branches could be used for health maintenance or diseases treatment with beneficial their effects without dyeing. As a beneficial effect, we reported accelerative effects on neurite outgrowth in PC12 cells [13,14].…”

mentioning

confidence: 86%

“…Dried branches of L. inermis were obtained as described in previous report [14]. A voucher of the plant is available in our laboratory [KPU-N.T.H LIB].…”

Section: Plant Materialmentioning

confidence: 99%

Anti-invasive Activity of Lawsonia inermis Branch and Its Potential Target Protein

Nakashima

Oda

Kishimoto

et al. 2018

Natural Product Communications

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The methanolic extract from the branch of Lawsonia inermis was found to inhibit the invasion of HT1080 cells strongly. The extract and its EtOAc-and 1-BuOH-soluble fractions strongly inhibited the enzyme activities and the secretions of MMPs from HT1080 cells. We, therefore, tried to examine and estimate the active constituents among the constituents isolated. However, there are no active compound among the isolated compounds. Next, we tried to identify the selective binding protein of whole constituents of their extracts. As a result, ribosomal protein S18 (RPs S18, human), 60S ribosomal protein L18 isoform 1 (60S RPs L18 i1, human) and eukaryotic elongation factor 2 (eEF2, human) were detected as their selective binding and potential target proteins. To confirm that the proteins are target proteins or not, we examined the effects of their proteins on synthesis of MMPs using siRNA method. With the treatment of siRNA for RPs S18, 60S RPs L18 i1 and eEF2 proteins, the MMP-2 protein synthesis were downregulated in HT1080 cells. MMP-9 productions were also downregulated with the treatment of siRNA for RPs S18 and eEF2. From the results, we reached a conclusion that the extract was considered to have the potential to inhibit the production of MMPs proteins via inhibition of RPs S18 and eEF2 proteins, leading to inhibit the invasion in HT1080 cells.

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Comparison of lawsone contents among Lawsonia inermis plant parts and neurite outgrowth accelerators from branches

Cited by 24 publications

References 21 publications

A Review on Lawsonia Inermis: A Potential Medicinal Plant

A Review on Lawsonia Inermis: A Potential Medicinal Plant

Effects of Naphthazarin (DHNQ) Combined with Lawsone (NQ-2-OH) or 1,4-Naphthoquinone (NQ) on the Auxin-Induced Growth of Zea mays L. Coleoptile Segments

Anti-invasive Activity of Lawsonia inermis Branch and Its Potential Target Protein

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