Comparison of Lactic Acid Bacteria Fermentation with Acid Treatments for Chitosan Production from Shrimp Waste

Phuvasate, Sureerat; Su, Yi‐Cheng

doi:10.1080/10498850.2010.504324

Cited by 12 publications

(3 citation statements)

References 20 publications

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“…The combination of Plackett-Burman, path of steepest ascent, and Box-Behnken design was effective and reliable in selecting the statistically significant factors and optimal levels of those factors for the highest yield and T-AO activity of crude EPS in this study. The shrimp byproduct medium contains large amounts of nutritive components [ 26 ] and has been fermented by lactic acid bacteria to recover chitosan and chitin [ 27 , 28 ]. However, the shrimp byproduct is subalkaline [ 27 ]; therefore, glacial acetic acid (about 1%) was needed to begin fermentation.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Cultural Conditions for Antioxidant Exopolysaccharides fromXerocomus badiusGrown in Shrimp Byproduct

Gao

Yan

Liu

et al. 2016

BioMed Research International

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To optimize the production conditions for exopolysaccharides with higher antioxidant activities from Xerocomus badius cultured in shrimp byproduct medium, Plackett-Burman design, path of steepest ascent, and response surface methodology were explored. Based on the results of Plackett-Burman design and path of steepest ascent, a Box-Behnken design was applied to optimization and the regression models. The optimal cultural condition for high yield and antioxidant activity of the exopolysaccharides was determined to be 10.347% of solid-to-liquid ratio, a 4.322% content of bran powder, and a 1.217% concentration of glacial acetic acid. Culturing with the optimal cultural conditions resulted in an exopolysaccharides yield of 4.588 ± 0.346 g/L and a total antioxidant activity of 2.956 ± 0.105 U/mg. These values are consistent with the values predicted by the corresponding regression models (RSD < 5%).

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Section: Resultsmentioning

confidence: 99%

Optimization of Cultural Conditions for Antioxidant Exopolysaccharides fromXerocomus badiusGrown in Shrimp Byproduct

Gao

Yan

Liu

et al. 2016

BioMed Research International

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“…Morphologically distinct colonies were sub-cultured and maintained on LSP agar plates. Strains of Bacillus subtilis , Pseudomonas fluorescens , Trichoderma harzianum , and Lactobacillus acidophilus were previously studied for crustacean shell waste degradation ( Sini et al, 2007 ; Phuvasate and Su, 2010 ; Das et al, 2012 ; Lee and Kim, 2015 ). These microbes (revived from laboratory stock cultures; B. subtilis NRS 231, P. fluorescens 271, T. harzianum AB 63-3 and L. acidophilus Scav) were screened for lobster shell degradation activity along with the soil isolates.…”

Section: Methodsmentioning

confidence: 99%

Microbial Degradation of Lobster Shells to Extract Chitin Derivatives for Plant Disease Management

et al. 2017

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Biodegradation of lobster shells by chitinolytic microorganisms are an environment safe approach to utilize lobster processing wastes for chitin derivation. In this study, we report degradation activities of two microbes, “S223” and “S224” isolated from soil samples that had the highest rate of deproteinization, demineralization and chitinolysis among ten microorganisms screened. Isolates S223 and S224 had 27.3 and 103.8 protease units mg-1 protein and 12.3 and 11.2 μg ml-1 of calcium in their samples, respectively, after 1 week of incubation with raw lobster shells. Further, S223 contained 23.8 μg ml-1 of N-Acetylglucosamine on day 3, while S224 had 27.3 μg ml-1 on day 7 of incubation with chitin. Morphological observations and 16S rDNA sequencing suggested both the isolates were Streptomyces. The culture conditions were optimized for efficient degradation of lobster shells and chitinase (∼30 kDa) was purified from crude extract by affinity chromatography. The digested lobster shell extracts induced disease resistance in Arabidopsis by induction of defense related genes (PR1 > 500-fold, PDF1.2 > 40-fold) upon Pseudomonas syringae and Botrytis cinerea infection. The study suggests that soil microbes aid in sustainable bioconversion of lobster shells and extraction of chitin derivatives that could be applied in plant protection.

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“…Shrimp industry generates large amounts of shrimp shells which account for about 40% of the shrimp weight. They are rich in protein, chitin, lipids, and carotenoid (Phuvasate & Su, 2010).…”

Section: Introductionmentioning

confidence: 99%

Chitin extraction from shrimp wastes by single step fermentation with Lactobacillus acidophilus FTDC3871 using response surface methodology

Tan

Abbasiliasi

Lee

et al. 2020

J. Food Process. Preserv.

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This study aimed to produce chitin and chitosan from shrimp wastes using lactic acid bacteria (LAB). From four strains of LAB and their mixture, Lactobacillus acidophilus FTDC3871 was selected due to higher proliferation and lactic acid production. The fermentation parameters influencing on chitin and chitosan yields such as glucose concentration (w/w), inoculum size (v/w), fermentation time (h), and water content (mL) were optimized using response surface methodology (RSM) with central composite design (CCD). The optimum levels for glucose concentration, inoculum size and water content were 15% (w/w), 5% (v/w), and 100 ml after 72 hr of incubation at 37°C. These conditions led to 90.8% of demineralization and 76% of deproteinization which resulted in 57.7% of chitin yield. The highest concentrations of carotenoid extracted and lactic acid accumulated from hydrolysate were 183.9 μg/g and 21.3 g/L, respectively. Results showed potential application of L. acidophilus FTDC3871 in chitin extraction from shrimp waste. Practical applications In this study, fermenting of shrimp wastes with lactic acid bacteria (LAB) was explored to solve the problems in conventional purification processes. This method seems promising, since protein, chitin, and carotenoid could be obtained simultaneously and eco-friendly. Since the efficiency of fermentation process depends on factors such as fermenting strains, inoculum size, carbon source concentration, initial pH and pH evolution during fermentation, and fermentation duration, therefore, a suitable LAB strain was selected to improve the fermentation process by optimizing above factors which influence on chitin and chitosan yields using a statistical approach, response surface methodology (RSM). How to cite this article: Tan JS, Abbasiliasi S, Lee CK, Phapugrangkul P. Chitin extraction from shrimp wastes by single step fermentation with Lactobacillus acidophilus FTDC3871 using response surface methodology. J Food

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Comparison of Lactic Acid Bacteria Fermentation with Acid Treatments for Chitosan Production from Shrimp Waste

Cited by 12 publications

References 20 publications

Optimization of Cultural Conditions for Antioxidant Exopolysaccharides fromXerocomus badiusGrown in Shrimp Byproduct

Optimization of Cultural Conditions for Antioxidant Exopolysaccharides fromXerocomus badiusGrown in Shrimp Byproduct

Microbial Degradation of Lobster Shells to Extract Chitin Derivatives for Plant Disease Management

Chitin extraction from shrimp wastes by single step fermentation with Lactobacillus acidophilus FTDC3871 using response surface methodology

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