Optimization of Cultural Conditions for Antioxidant Exopolysaccharides fromXerocomus badiusGrown in Shrimp Byproduct

Abstract

To optimize the production conditions for exopolysaccharides with higher antioxidant activities from Xerocomus badius cultured in shrimp byproduct medium, Plackett-Burman design, path of steepest ascent, and response surface methodology were explored. Based on the results of Plackett-Burman design and path of steepest ascent, a Box-Behnken design was applied to optimization and the regression models. The optimal cultural condition for high yield and antioxidant activity of the exopolysaccharides was determined to be 10.347% of solid-to-liquid ratio, a 4.322% content of bran powder, and a 1.217% concentration of glacial acetic acid. Culturing with the optimal cultural conditions resulted in an exopolysaccharides yield of 4.588 ± 0.346 g/L and a total antioxidant activity of 2.956 ± 0.105 U/mg. These values are consistent with the values predicted by the corresponding regression models (RSD < 5%).

Optimization of the culture condition of Bacillus mucilaginous using Agaricus bisporus industrial wastewater by Plackett–Burman combined with Box–Behnken response surface method

“…The experimental design included 6 factors and the experimental number selected was N = 12. A, B, C, D, E, and F represents precooked liquid solubility, initial pH, inoculum size, culture temperature, shaking speed, and liquid volume, respectively (Chen et al 2015a , 2017a ; Gao et al 2016 ). Based on the results of the single-factor experiments, each factor was taken to two levels (Table 2 ).…”

“…The steepest ascent method changes the gradient direction of the experimental value into the direction of hill climbing. Based on the effect of each factor, the change step size can be determined, which can be used to quickly and economically approximate the optimal value area (Chen et al 2015a ; Gao et al 2016 ). According to the size of the key factor effect value in the Plackett–Burman experiment, the change distance and direction of climbing can be determined.…”

Response surface optimization of conditions for culturing <i>Azotobacter chroococcum</i> in <i>Agaricus bisporus</i> industrial wastewater

“…C, F, and I were set as blank terms to estimate the experimental error. A, B, D, E, G, and H represented precooked liquid solubility, initial pH, inoculum size, liquid volume, temperature, and shaking speed, respectively (Chen et al, 2015Gao et al, 2016). Based on the results of the single-factor experiments, each factor was taken to one high level (+1) and one low level (-1).…”

“…The steepest ascent method changes the gradient direction of the experimental value into the direction of hill climbing. Based on the effect of each factor, the change step size can be determined, which can be used to quickly and economically approximate the optimal value area (Chen et al, 2015;Gao et al, 2016). According to the size of the key factor effect value in the Plackett-Burman experiment, the change distance and direction of climbing can be determined.…”

Identification and Functional Mechanism of Novel Angiotensin I Converting Enzyme Inhibitory Dipeptides from Xerocomus badius Cultured in Shrimp Processing Waste Medium

“…However, there has been few reports about the ACE inhibitory peptides from liquid fermented products of medicinal fungi, although it has the advantages of lowering the cost, making industrialization and control of product quality easier. In our previous studies, shrimp processing waste was fermented by Xerocomus badius and Angiotensin I converting enzyme (ACE) inhibitory fragments were obtained from the resulting products in the process of recovery chitin and antioxidant polysaccharides [ 20 – 22 ].…”