Comparison of intradermal and serum testing for allergen-specific IgE using a FcεRIα-based assay in atopic dogs in the UK

Foster, Aiden P; Littlewood, Janet D.; Webb, Paul W.; Wood, J. L. N.; Rogers, Katherine H.; Shaw, Susan E.

doi:10.1016/s0165-2427(03)00052-7

Cited by 57 publications

(60 citation statements)

References 33 publications

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“…Unlike human sensitisations, dogs are more sensitised to D. farinae than to D. pteronyssinus as reported in all European studies (Carlotti and Costargent, 1992;Sture et al, 1995, Saridomichelakis et al, 1999Bensignor and Carlotti, 2002;Foster et al, 2003;Tarpataki et al, 2006), in conformity with our results. The ratio between sensitisations to D. farinae and D. pteronyssinus varies greatly among studies, ranging from 1.06 to 5.37, mainly when sensitisations to D. pteronyssinus are low.…”

Section: Discussionsupporting

confidence: 93%

“…Our results show a significant correlation between D. farinae and D. pteronyssinus, similar to the OD correlation reported by Foster et al (2003) using the same test (ALLERCEPT). Nevertheless, the correlation between these two mites was found to be weaker than that reported in studies of humans (Vidal et al, 1997).…”

Section: Discussionsupporting

confidence: 91%

“…The ratio between sensitisations to D. farinae and D. pteronyssinus varies greatly among studies, ranging from 1.06 to 5.37, mainly when sensitisations to D. pteronyssinus are low. Our results showed a ratio of 2.02, which is markedly lower than that reported by Foster et al (2003) using the same serological test. The explanation for these differences could be the modifications made to the OD cut-off of the test (from 350 EA units to 150 EA units) in recent years, which will have probably increased the sensitivity of the test for both allergens, revealing an increase in positive results for D. pteronyssinus.…”

Section: Discussioncontrasting

confidence: 90%

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Importance of house dust and storage mites in canine atopic dermatitis in the geographic region of Galicia, Spain

Goicoa

Espino

Rodriguez

et al. 2008

Acta Veterinaria Hungarica

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Sensitisation to mites is frequent in atopic dogs. The main mite genus involved in canine atopic dermatitis is Dermatophagoides. The importance of storage mite allergens in dogs has been controversial. The aim of this study was to evaluate the sensitisation rates against storage mites (Lepidoglyphus destructor and Tyrophagus putrescentiae) and house dust mites (Dermatophagoides farinae and D. pteronyssinus) in atopic dogs from Galicia, a highly humid and temperate region of Spain, using a FcεRIα-based immunoglobulin E (IgE) in vitro test. The study was performed on 95 dogs suffering from atopic dermatitis and presenting detectable specific serum IgE levels: 91.6% of the dogs tested positive for storage mites, whereas sensitisation to house dust mites was detected in 87.4%. These results indicate the importance of storage mites in this specific geographic area.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 90%

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Importance of house dust and storage mites in canine atopic dermatitis in the geographic region of Galicia, Spain

Goicoa

Espino

Rodriguez

et al. 2008

Acta Veterinaria Hungarica

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“…Multiple in vitro assays for detection of allergen-specific IgE are available in the veterinary arena [9,12,16,17,20,23,26], yet there is a paucity of useful data describing the assays and comparing the available tests. The best characterized assays for detection of allergen-specific IgE in dogs are a monoclonal antibody cocktail-based ELISA for dog specific IgE [17] and a human high affinity IgE receptor based assay for dog IgE [6,27]. When directly compared, the concordance of results for these 2 assays was demonstrated to be approximately 92% which is similar to the intra-ELISA/inter-laboratory concordance of results [17].…”

mentioning

confidence: 76%

Measurement for Canine IgE Using Canine Recombinant High Affinity IgE Receptor α Chain (FcεRIα)

Tsukui

Sakaguchi

Kurata³

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. To detect allergen-specific IgE in dogs with allergic diseases, we developed a recombinant canine high affinity IgE receptor α chain (FcεRIα)-based IgE detection system. Using the recombinant protein of canine FcεRIα expressed by an Escherichia coli expression system, we could detect house dust mite (Dermatophagoides farinae) allergen-specific IgE in sera from dogs naturally and experimentally sensitized to this allergen with ELISA and western blotting. The IgE binding activity of recombinant canine FcεRIα on ELISA was impaired by heat treatment of these sera. The specificity of this recombinant canine FcεRIα-based IgE detection system was confirmed by inhibition assays with canine IgE. The recombinant canine FcεRIα-based IgE detection system established in this study offers an alternative tool to measure allergen-specific IgE in dogs.

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“…To examine the FcRI expression on cultured cells, the cells were incubated with pooled serum derived from dogs that had been experimentally hypersensitized with Japanese cedar pollen as reported previously [43]. The titer of IgE specific to Japanese cedar pollen antigen in the pooled serum was shown to be more than 2,500 laboratory units by FcRI-based ELISA (Allercept ® Definitive Allergen Panels, Heska, Ft Collins, CO, U.S.A.) [9]. Cells were incubated with 10% of the IgE-rich dog serum and 0.25 g/ml ethidium monoazide (EMA; Molecular Probes, Eugene, OR, U.S.A.) in a wash buffer (PBS containing 2% FBS) for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Cultivation and Characterization of Canine Skin-Derived Mast Cells

Kawarai

Masuda

Ohmori

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. It is essential to develop a technique to culture purified skin-derived mast cells (SMCs) to facilitate immunological research on allergic diseases in dogs. This study was performed to develop an efficient culture system for canine SMCs and to characterize the cells in comparison to canine bone marrow-derived mast cells (BMMCs). Enzymatically digested skin biopsy samples were cultivated in serum-free AIM-V medium supplemented with recombinant canine stem cell factor. Three to five weeks after the initiation of culture, mast cells were collected by a magnetic activated cell separation system using anti-c-Kit antibody. The collected cells were composed of a uniform population showing morphological characteristics of mast cells with a round or oval nucleus and abundant toluidine bluepositive metachromatic granules in the cytoplasm. The results of flow cytometric analysis for the presence of cell membrane c-Kit and Fc epsilon receptor I (FcRI) indicated that approximately 90% of the cells were mast cells. The cytoplasmic granules were positive for both tryptase and chymase. Apparent dose-dependent degranulation was induced by antibody-mediated cross-linking of immunoglobulin E (IgE) bound to the cells. These cytological and immunological characteristics observed in SMCs were mostly similar to those observed in BMMCs; however, IgE-mediated degranulation was significantly lower in SMCs than BMMCs. The culture system for canine SMCs developed in this study would be useful in understanding the pathophysiology and developing anti-allergic therapeutics in canine allergic dermatitis.KEY WORDS: bone marrow, canine, culture, mast cells, skin.J. Vet. Med. Sci. 72(2): 131-140, 2010 Allergic dermatitis in dogs is a complex of inflammatory skin diseases primarily mediated by type I hypersensitivity [8]. Cross-linking of the surface Fc epsilon receptor I (FcRI)-bound immunoglobulin E (IgE) with allergens results in the release of various mediators from mast cell granules, inducing type I hypersensitivity [5,6]. Studies using an experimental allergic model have revealed that cytokines and chemokines released from mast cells induce infiltration of leukocytes, leading to a late-phase reaction [42].In dogs, histopathological examination of the skin lesions of atopic dermatitis (AD) has revealed infiltration of mast cells, Langerhans cells, and lymphocytes in addition to a small number of eosinophils and neutrophils into the lesional skin [14]. The presence of allergen-specific IgEbearing mast cells in the dermis of allergic dogs has been shown [13], and both the number of mast cells and the histamine concentration in the dermis have been shown to correlate with clinical symptoms of AD [28]. As shown in the spontaneously developed AD in dogs, in a canine experimental model of AD, epicutaneous application of an allergen slurry has been shown to generate a penetration of allergen into the dermis, an elevated allergen-specific serum IgE, and localized pruritic dermatitis with typical histological changes by IgE-induced inf...

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Comparison of intradermal and serum testing for allergen-specific IgE using a FcεRIα-based assay in atopic dogs in the UK

Cited by 57 publications

References 33 publications

Importance of house dust and storage mites in canine atopic dermatitis in the geographic region of Galicia, Spain

Importance of house dust and storage mites in canine atopic dermatitis in the geographic region of Galicia, Spain

Measurement for Canine IgE Using Canine Recombinant High Affinity IgE Receptor α Chain (FcεRIα)

Cultivation and Characterization of Canine Skin-Derived Mast Cells

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