Comparison of Infectivity of Strains of Trypanosoma cruzi (Chagas, 1909)

Bice, David E.; Zeledon, Rodrigo

doi:10.2307/3277711

Cited by 78 publications

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“…Studies of this parasite strains conducted since 1950 have shown that T. cruzi can be found in different vectors and reservoirs and that parasites isolated from different regions of the same country behave differently when inoculated into experimental models [36][37] .…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Gómez-Hernández

Rezende-Oliveira

Nascentes

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. Methods: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD -(random amplified polymorphic DNA). Results: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10 6 to 10 7 parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. Conclusions: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Keywords: Trypanosoma cruzi. Mexico. Biological characterization. Genetic characterization. RESUMOIntrodução: Durante muito tempo, foi questionada a importância da doença de Chagas no México onde muitos a consideravam um padecimento exótico. Considerando a grande diversidade genética existente, entre os isolados de Trypanosoma cruzi, a importância da caracterização biológica desses e o escasso número de informações sobre os aspectos clínicos e biológicos da doença de Chagas no México, o objetivo deste trabalho foi realizar a caracterização biológica e molecular de isolados de Trypanosoma cruzi originários de diferentes áreas endêmicas deste país, principalmente do Estado de Jalisco. Métodos: Oito cepas mexicanas de Trypanosoma cruzi foram caracterizadas biologicamente e geneticamente (PCR específica para Trypanosoma cruzi, PCR-multiplex, amplificação do espaço não transcrito dos genes do mini-exon, amplificação das regiões polimórficas do gene do mini-exon, classificação pela amplificação de regiões intergênicas dos genes do spliced leader, RAPD -random amplified polymorphic DNA). Resultados: Foram observados dois tipos de parasitemia: patente com picos máximos de parasitemia entre 4,6x10 6 e 10 7 parasitas/mL e subpatente. Além disso, todos os isolados foram capazes de infectar 100% dos animais. Observou-se tropismo predominante pelo músculo estriado (cardíaco e esquelético). As técnicas de PCR do gene do mini-éxon classificaram as oito cepas como TcI e a técnica de RAPD mostrou variaçã...

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Gómez-Hernández

Rezende-Oliveira

Nascentes

et al. 2011

Rev. Soc. Bras. Med. Trop.

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“…The urogenital organs are regularly parasitized in experimental infections (Bice & Zeledón 1970, Herrera & Urdaneta-Morales 1992, Tavares et al 1994, Lenzi et al 1996, Scorza et al 1996. Blood trypomastigotes have been isolated from the urine of opossums and mice (references in Zeledón 1974).…”

Section: Discussionmentioning

confidence: 99%

Experimental transmission of Trypanosoma cruzi through the genitalia of albino mice

Herrera

Urdaneta‐Morales

2001

Mem. Inst. Oswaldo Cruz

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“…This difference may be due to the abundance of smooth muscle in the first two sites, since muscular tissue constitutes one of the preferential targets for T. cruzi infection (Bice & Zeledon 1970, Andrade & Andrade 1979.…”

Section: Cmentioning

confidence: 99%

Genitourinary changes in hamsters infected and reinfected with Trypanosoma cruzi

Cabrine-Santos

Santos

Lima

et al. 2003

Mem. Inst. Oswaldo Cruz

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Comparison of Infectivity of Strains of Trypanosoma cruzi (Chagas, 1909)

Cited by 78 publications

References 0 publications

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Experimental transmission of Trypanosoma cruzi through the genitalia of albino mice

Genitourinary changes in hamsters infected and reinfected with Trypanosoma cruzi

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