Comparison of in vivo1H MRS of human brain tumours with1H HR-MAS spectroscopy of intact biopsy samples in vitro

Barton, Sarah J.; Howe, Franklyn A.; Tomlins, Andrew M.; Cudlip, Simon; Nicholson, Jeremy K.; Bell, B. Anthony; Griffiths, John R.

doi:10.1007/bf02590529

Cited by 59 publications

(34 citation statements)

References 19 publications

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“…In tumours, Cho et al [18] found a rise in Myo/Cho in schwannomas. Comparing cerebral tumours, various authors observed a diminution of the ratio Myo/Cr with an augmentation of the tumour grade [5, 33, 52, 56]. There have been contradictory results, showing an increase in Myo or Myo/Cr with an augmentation of the tumour grade, but without a significant correlation [19, 20, 30].…”

Section: Discussionmentioning

confidence: 97%

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Monovoxel <sup>1</sup>H Magnetic Resonance Spectroscopy in the Progression of Gliomas

et al. 2007

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Aim: Can monovoxel magnetic resonance spectroscopy (MRS) reliably follow tumour progression in low-grade glioma? Materials and Methods: 21 patients with low-grade glioma underwent at least 3 MRS. Results: For progression from a grade II to grade III tumour, a sensitivity of 57.1% and specificity of 60% were observed, with a positive predictive value (PPV) of 48.8% and a negative predictive value (NPV) of 54.5%. For progression under treatment, we obtained a sensitivity of 57.1% by N-acetylaspartate (NAA)/choline (Cho) and myoinositol/creatine (Cr) and a specificity of 100% by Cho/Cr and lipids, with a PPV of 80% and a NPV of 63.6%. Conclusion: We found that NAA/Cho is the best marker of tumour progression before therapy, with a sensitivity of 53.9%. For the therapeutic response, sensitivity was only 28.2%.

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Section: Discussionmentioning

confidence: 97%

“…The contribution of glycine to the signal is small [52]. In demyelinating diseases, it was found to increase, corresponding to myelin breakup [32].…”

Section: Discussionmentioning

confidence: 99%

Monovoxel <sup>1</sup>H Magnetic Resonance Spectroscopy in the Progression of Gliomas

et al. 2007

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“…Introduction Recent work has suggested that magnetic resonance spectroscopy (MRS) may be useful in evaluating cancerous lesions [1]. MRS data have been obtained from a variety of body organs including the brain [2,3]. In a brain tumour resection, many (> 12) biopsy samples may be taken.…”

Section: Related Contentmentioning

confidence: 99%

Micro-coils for MR spectroscopy by deep silicon etching

Syms¹,

Ahmad²,

Young³

et al. 2005

J. Phys.: Conf. Ser.

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A process for batch fabrication of low-cost microcoils for magnetic resonance spectroscopy is demonstrated. Conductors are fabricated on oxidised silicon substrates by electroplating metals inside a deep photoresist mould, and passivated using an epoxy-based resist. Through-wafer deep reactive ion etching is used to define sample volumes and stencil cuts around each die, and dies are separated by snap-out. Single-coil and multiple-coil sensors are constructed by stacking parts on baseplates fabricated on the same wafer. Single-turn coils have a Q-factor of ≈ 15 at 63.6 MHz, and Helmholtz coils a Q-factor of ≈ 13. Magnetic resonance imaging and spectroscopy are performed, and a SNR of 900 is achieved.

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“…One of the major applications of tissue analysis by HR-MAS NMR spectroscopy is the diagnosis and monitoring of cancer. 16,21,23,24 Tumor samples often contain elevated lipid concentrations compared to healthy controls, 23,24 which are not always detected in cancer cell extracts. 24 Although tissue excision is required, the intra-and extracellular contents of cells are analyzed directly by HR-MAS avoiding the risk of losing important bioanalytical information because of extraction or dilution of this information because of systemic transport.…”

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confidence: 99%

Concentration Profiling in Rat Tissue by High-Resolution Magic-Angle Spinning NMR Spectroscopy: Investigation of a Model Drug

et al. 2005

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The utility of high-resolution magic-angle spinning (HR-MAS) NMR for studying drug delivery in whole tissues was explored by dosing female Sprague-Dawley rats with topical or injectable benzoic acid (BA). In principle, HR-MAS NMR permits the detection of both intra-and extracellular compounds. This is an advantage over the previous detection of topically applied BA using microdialysis coupled to HPLC/UV as microdialysis samples only the extracellular space. Skin and muscle samples were analyzed by 1 H HR-MAS NMR, and BA levels were determined using an external standard solution added to the sample rotor. One to two percent of the BA topical dose was detected in the muscle, showing that BA penetrated through the dermal and subcutaneous layers. Since BA was not detected in the muscle in the microdialysis studies, the NMR spectra revealed the intracellular localization of BA. The amount of BA detected in muscle after subcutaneous injection correlated with the distance from the dosing site. Overall, the results suggest that HR-MAS NMR can distinguish differences in the local concentration of BA varying with tissue type, dosage method, and tissue proximity to the dosing site. The results illustrate the potential of this technique for quantitative analysis of drug delivery and distribution and the challenges to be addressed as the method is refined.Nuclear magnetic resonance (NMR) spectroscopy has played a significant role in the growth of metabonomics, an area of clinical research that aims to measure the total metabolic response of an organism to a stimulus such as a drug or toxicant or a physiological change such as a disease state. 1-3 The effects of the stimulus can be evaluated by measuring levels of specific analytes or through pattern recognition to permit the identification of a fingerprint of efficacy or toxicity. 1-3 Spectral analysis of the individual components that comprise the fingerprint reveals the identity of drug metabolites 4 and disease biomarkers. 5,6 The major advantages of NMR spectroscopy for metabonomics studies over other techniques, such as mass spectrometry, 7,8 are the universal nature of NMR detection, ease of quantitation, and the ability to analyze both biofluids and tissues to understand the local versus systemic effects of a stimulus. 9,10 Such an integrated metabonomics approach is an ongoing focus of this collaborative effort. Although NMR is generally less sensitive than other spectroscopic techniques, recent technological developments have reduced sample mass requirements and experiment times significantly. 11,12Tissue analysis by NMR spectroscopy is possible with a technique known as high-resolution magic-angle spinning (HR-MAS Another potential, but relatively unexplored, application of HR-MAS NMR tissue analysis is for drug delivery and distribution studies. Formulation is a critical part of the drug development process affecting the ADME (absorption, distribution, metabolism, and excretion) properties of a drug. While oral formulation is the preferred route for the ad...

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Comparison of in vivo1H MRS of human brain tumours with1H HR-MAS spectroscopy of intact biopsy samples in vitro

Cited by 59 publications

References 19 publications

Monovoxel <sup>1</sup>H Magnetic Resonance Spectroscopy in the Progression of Gliomas

Monovoxel <sup>1</sup>H Magnetic Resonance Spectroscopy in the Progression of Gliomas

Micro-coils for MR spectroscopy by deep silicon etching

Concentration Profiling in Rat Tissue by High-Resolution Magic-Angle Spinning NMR Spectroscopy: Investigation of a Model Drug

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