Comparison of in vitro biocompatibility of NanoBone® and BioOss® for human osteoblasts

Liu, Qin; Douglas, Timothy; Zamponi, Christiane; Becker, Stephan; Sherry, Eugene; Sivananthan, Sureshan; Warnke, Frauke; Wiltfang, Joerg; Warnke, Patrick H.

doi:10.1111/j.1600-0501.2010.02100.x

Cited by 43 publications

(31 citation statements)

References 9 publications

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“…This is in agreement with the previously published literature where it has been shown that human osteosarcoma cell lines, osteoblasts [16], bone marrow derived mesenchymal stem cells [32], and human tooth germ cells [33] are able to adhere and grow on MTA and similarly osteoblasts [34], mesenchymal stem cells from peripheral blood [35], or mesenchymal stem cells from bone marrow [36] can grow on Bio-Oss. Moreover, in the present study, we were able to directly compare the three materials and demonstrate for the first time that dental stem cells seemed to prefer dentin chips and MTA as opposed to Bio-Oss.…”

Section: Discussionsupporting

confidence: 92%

Dental Stem Cell Migration on Pulp Ceiling Cavities Filled with MTA, Dentin Chips, or Bio-Oss

Lymperi

Taraslia

Tsatsoulis

et al. 2015

BioMed Research International

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MTA, Bio-Oss, and dentin chips have been successfully used in endodontics. The aim of this study was to assess the adhesion and migration of dental stem cells on human pulp ceiling cavities filled with these endodontic materials in an experimental model, which mimics the clinical conditions of regenerative endodontics. Cavities were formed, by a homemade mold, on untouched third molars, filled with endodontic materials, and observed with electron microscopy. Cells were seeded on cavities' surface and their morphology and number were analysed. The phenomenon of tropism was assessed in a migration assay. All three materials demonstrated appropriate microstructures for cell attachment. Cells grew on all reagents, but they showed a differential morphology. Moreover, variations were observed when comparing cells numbers on cavity's filling versus the surrounding dentine disc. The highest number of cells was recorded on dentin chips whereas the opposite was true for Bio-Oss. This was confirmed in the migration assay where a statistically significant lower number of cells migrated towards Bio-Oss as compared to MTA and dentin chips. This study highlights that MTA and dentin chips have a greater potential compared to Bio-Oss regarding the attraction of dental stem cells and are good candidates for bioengineered pulp regeneration.

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Section: Discussionsupporting

confidence: 92%

Dental Stem Cell Migration on Pulp Ceiling Cavities Filled with MTA, Dentin Chips, or Bio-Oss

Lymperi

Taraslia

Tsatsoulis

et al. 2015

BioMed Research International

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“…Unlikely to our study, where no difference in the cell number between materials was found in the study by Warnke et al significantly lower number of cells in the presence of Bio Oss was recorded. Furthermore, comparing the adhesion and quantity of human osteoblasts cultured on “golden standard” Bio Oss and synthetic bone substitute—Nano Bone for 7 days, more cells with cytoplasmic extensions were observed in the presence of Nano Bone . Hence, there are many scaffolds including the ones used in our study which exhibit good biocompatibility and could replace materials currently used in tissue engineering.…”

Section: Discussionmentioning

confidence: 83%

Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells

Karadžić

Vučić

Jokanovic

et al. 2014

J. Biomed. Mater. Res.

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The aim of this study was to examine the differential capacity of isolated dental pulp stem cells (SHED) cultured onto four different scaffold materials. The differential potential of isolated SHED was examined on the following scaffolds: porous hydroxyapatite (pHAP) alone or combined with three polymers [polylactic-co-glycolic acid (PLGA), alginate, and ethylene vinylacetate / ethylene vinylversatate (EVA/EVV)]. SHED were isolated by "outgrowth" method and characterized by the flow cytometry. Viability of cells grown with scaffolds was assessed by MTT and LDH assays. No significant cytotoxic effect of any of the tested materials was shown. Staining with alizarin red and estimated alkaline phosphatase activity to identify differentiation, demonstrated osteoblastic phenotype of SHED and newly deposited and mineralized extra cellular matrix (ECM) in presence of all tested scaffolds. The developed ECM seen at scanning electronic micrographs additionally confirmed the osteogenic differentiation and biocompatibility between cells and materials. In summary, all studied biomaterials are suitable carriers for proliferation and osteoblastic differentiation of dental pulp mesenchymal stem cells in vitro.

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“…Based on the findings of others, NHA1 supported and promoted proliferation of human osteoblasts slightly better than DBBM (Liu et al. ). Ghanaati et al.…”

Section: Discussionmentioning

confidence: 90%

Bone formation in mono cortical mandibular critical size defects after augmentation with two synthetic nanostructured and one xenogenous hydroxyapatite bone substitute – in vivo animal study

Dau

Kämmerer

Henkel

et al. 2015

Clinical Oral Implants Res

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Comparison of in vitro biocompatibility of NanoBone® and BioOss® for human osteoblasts

Cited by 43 publications

References 9 publications

Dental Stem Cell Migration on Pulp Ceiling Cavities Filled with MTA, Dentin Chips, or Bio-Oss

Dental Stem Cell Migration on Pulp Ceiling Cavities Filled with MTA, Dentin Chips, or Bio-Oss

Effects of novel hydroxyapatite-based 3D biomaterials on proliferation and osteoblastic differentiation of mesenchymal stem cells

Bone formation in mono cortical mandibular critical size defects after augmentation with two synthetic nanostructured and one xenogenous hydroxyapatite bone substitute – in vivo animal study

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