Comparison of in vitro and in situ plankton production determinations

Robinson, Carol; Tilstone, Gavin H.; Rees, Andrew P.; Smyth, Tim; Fishwick, James; Tarran, Glen A.; Luz, Boaz; Barkan, Eugeni; David, Efrat

doi:10.3354/ame01250

Cited by 47 publications

(78 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Lastly, there are no health hazards associated with using 18 O because it is a stable isotope. One disadvantage of this method is that gross oxygen production determined by the 18 O method evaluates total oxygen production and it is unclear if this is directly linked to (Bender et al, 1999;Robinson et al, 2009). Indeed, four different metabolic pathways mainly involve oxygen consumption in the light, respiration through the cytochrome oxidase pathway, respiration by the alternative oxidase pathway, photorespiration and the Mehler reaction (Robinson et al, 2009).…”

Section: An Overview Of Methods To Measure Marine Phytoplanktonic Primentioning

confidence: 99%

“…Whereas the majority of published studies evaluate the primary production of planktonic community using a single method, a number of reports using two or more techniques have now become available. Different approaches to measure the volumetric primary production have been compared in a series of papers examining the rates delivered by different methods for specific locations, cruises or sampling events (Williams et al, 1996;Marra, 2002;Corno et al, 2005;Juranek and Quay, 2005;Gazeau et al, 2007;González et al, 2008;Robinson et al, 2009). Most of these comparisons revealed differences among methods, varying in magnitude, attributable to differences in the specific components of primary production addressed by each method as well as to their inherent assumptions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparing marine primary production estimates through different methods and development of conversion equations

et al. 2014

View full text Add to dashboard Cite

Numerous studies have compared the rates of primary production using various techniques at specific locations and times. However, these comparisons are local and cannot be used to compare or scale rates of primary production using different methods across ocean basins or seasonal time scales. Here, we quantify the range in rates of primary production derived using different techniques and provide equations that allow conversions of estimates between different methods. We do so on the basis of a compilation of data on volumetric estimates of primary production rates concurrently estimated with at least two different methods. We observed that the comparison of estimates of marine phytoplankton primary production derived from different methods reveals very large variations between methods. The highest primary production estimates are derived using the 18 O method, which may provide the best and more generally applicable estimate of gross primary production (GPP). The regression equations presented in this work provide the best available approach to convert data across methods and therefore integrate and synthesize available and future data derived using different methods.

show abstract

Section: An Overview Of Methods To Measure Marine Phytoplanktonic Primentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparing marine primary production estimates through different methods and development of conversion equations

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Robinson et al (2009) observed 18 O-GOP : 14 C-PP ratios . 5 at a diatomdominated station in the Celtic Sea.…”

mentioning

confidence: 96%

“…Thus, the recommendation from this and similar recent studies (Juranek and Quay 2005;Robinson et al 2009;Quay et al 2010) is to adopt a multipronged approach to PP and NCP measurement utilizing a suite of methods, each with their own biases, that in combination should better constrain biological productivity rates in the coastal ocean.…”

mentioning

confidence: 99%

Biological production rates off the Southern California coast estimated from triple O₂ isotopes and O₂ : Ar gas ratios

Munro

Quay

Juranek

et al. 2013

Limnology & Oceanography

View full text Add to dashboard Cite

The isotopic composition of dissolved O 2 ( 17 D) and the biological O 2 saturation from O 2 : Ar ratios were measured in the surface ocean during six cruises off the coast of southern California from November 2005 to August 2008 to determine rates of gross oxygen production (GOP), net oxygen production (NOP), and the NOP : GOP ratio (a measure of potential export efficiency). 17 D-GOP estimates were consistently greater than on-deck incubation-based 14 C-primary production ( 14 C-PP) by a factor of 5.6 6 0.4 and greater than satellite PP estimates by a factor of 3.5 6 0.3 (mmol O 2 : mmol C). The 17 D-GOP to 14 C-PP factor was twice the expected factor of 2.7 determined from comparisons of incubation-based 18 O-GOP and 14 C-PP. The annual mean NOP : GOP ratio was 0.16 6 0.06, suggesting a potential export efficiency that is surprisingly similar to the open ocean using comparable methods.

show abstract

“…It is known that a considerable fraction of primary production may be lost directly as dissolved organic carbon in nutrientstressed conditions (Ogawa and Tanoue, 2003). Recently, a paper comparing 8 different methods of measuring primary production highlighted the role of dissolved organic matter, which may lead to experimental underestimates of 14 C NPP especially in the case of nutrient-stressed cells (Robinson et al, 2009). Our results indicate that considering this fraction when comparing with 14 C in situ primary production estimates considerably improves the results.…”

Section: Doc and Primary Productionmentioning

confidence: 99%

Skill assessment of the PELAGOS global ocean biogeochemistry model over the period 1980–2000

Vichi

Masina

2009

Biogeosciences

View full text Add to dashboard Cite

Abstract. Global Ocean Biogeochemistry General Circulation Models are useful tools to study biogeochemical processes at global and large scales under current climate and future scenario conditions. The credibility of future estimates is however dependent on the model skill in capturing the observed multi-annual variability of firstly the mean bulk biogeochemical properties, and secondly the rates at which organic matter is processed within the food web. For this double purpose, the results of a multi-annual simulation of the global ocean biogeochemical model PELAGOS have been objectively compared with multi-variate observations from the last 20 years of the 20th century, both considering bulk variables and carbon production/consumption rates. Simulated net primary production (NPP) is comparable with satellite-derived estimates at the global scale and when compared with an independent data-set of in situ observations in the equatorial Pacific. The usage of objective skill indicators allowed us to demonstrate the importance of comparing like with like when considering carbon transformation processes. NPP scores improve substantially when in situ data are compared with modeled NPP which takes into account the excretion of freshly-produced dissolved organic carbon (DOC). It is thus recommended that DOC measurements be performed during in situ NPP measurements to quantify the actual production of organic carbon in the surface ocean. The chlorophyll bias in the Southern Ocean that affects this model as well as several others is linked to the inadequate representation of the mixed layer seasonal cycle in the region. A sensitivity experiment confirms that the artificial increase of mixed layer depths towards the observed values substantially reduces the bias. Our assessment resultsCorrespondence to: M. Vichi (vichi@bo.ingv.it) qualify the model for studies of carbon transformation in the surface ocean and metabolic balances. Within the limits of the model assumption and known biases, PELAGOS indicates a net heterotrophic balance especially in the more oligotrophic regions of the Atlantic during the boreal winter period. However, at the annual time scale and over the global ocean, the model suggests that the surface ocean is close to a weakly positive autotrophic balance in accordance with recent experimental findings and geochemical considerations.

show abstract

Comparison of in vitro and in situ plankton production determinations

Cited by 47 publications

References 89 publications

Comparing marine primary production estimates through different methods and development of conversion equations

Comparing marine primary production estimates through different methods and development of conversion equations

Biological production rates off the Southern California coast estimated from triple O₂ isotopes and O₂ : Ar gas ratios

Skill assessment of the PELAGOS global ocean biogeochemistry model over the period 1980–2000

Contact Info

Product

Resources

About

Comparison of in vitro and in situ plankton production determinations

Cited by 47 publications

References 89 publications

Comparing marine primary production estimates through different methods and development of conversion equations

Comparing marine primary production estimates through different methods and development of conversion equations

Biological production rates off the Southern California coast estimated from triple O2 isotopes and O2 : Ar gas ratios

Skill assessment of the PELAGOS global ocean biogeochemistry model over the period 1980–2000

Contact Info

Product

Resources

About

Biological production rates off the Southern California coast estimated from triple O₂ isotopes and O₂ : Ar gas ratios