Comparison of immunoreactivities in 4-HNE and superoxide dismutases in the cervical and the lumbar spinal cord between adult and aged dogs

Ahn, Ji Hyeon; Choi, Jung Hoon; Kim, Jin Sang; Lee, Hyun Joo; Lee, Choong Hyun; Yoo, Ki‐Yeon; Hwang, In Koo; Lee, Yun Lyul; Shin, Hyung‐Cheul; Won, Moo‐Ho

doi:10.1016/j.exger.2011.03.001

Cited by 15 publications

(18 citation statements)

References 40 publications

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“…In both adult and aged groups, we found that the staining pattern and number of NeuN (a marker for neurons) immunoreactive neurons were similar in both groups [27][28][29].…”

Section: Neun Immunoreactivitymentioning

confidence: 78%

“…Similarly, in other organs such as the heart, the amount of caspase-dependent (120-kDa) form of aII-spectrin cleavage is 17% higher in 36-month hearts than in the 6-month hearts of F344XBN rats [2]. Recently, we did not observe any significant changes in the number of NeuN-positive neurons in the gray matter of the spinal cord between adult and aged dogs [27][28][29]. However, it was reported that microtubule-associated protein 2 (MAP2), a cytoskeleton, was age-dependently decreased in the medial prefrontal cortex in the aging model such as senescence-accelerated mouse prone 10 strain and MAP2 immunoreactive and levels were decreased in the spinal cord of the aged dog [12,39].…”

Section: Discussionmentioning

confidence: 94%

“…These animals, which have given three paper [27][28][29], did not show any clinical nor any other signs in neural disorders. The handling and the care of the animals conformed to the guidelines established in order to comply with current international laws and policies (NIH Guide for the Care and Use of Laboratory Animals, NIH Publication No.…”

Section: Experimental Animalsmentioning

confidence: 88%

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Increased Immunoreactivities of Cleaved αII-Spectrin and Cleaved Caspase-3 in the Aged Dog Spinal Cord

et al. 2011

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The activation of caspase-3 is considered to be a reliable marker for apoptotic cell death, and a 120-kDa fragment of αII-spectrin is generated by caspase-3 mediated cleavage of this structural protein. In the present study, we compared cleaved αII-spectrin (120-kDa) and cleaved caspase-3-immunoreactive cells and their protein levels in the cervical (C₅-C₆) and lumbar (L₃-L₄) levels of the spinal cord in adult (1-2 year-old) and aged (10-12 year-old) dogs (German shepherds). Weak cleaved αII-spectrin and cleaved caspase-3 immunoreactivity was found in neurons of the adult group; however, their immunoreactivity was distinctively increased in the neuronal cytoplasm in the aged group compared to those in the adult group, although the distribution pattern of their neurons was similar between the adult and age group. In addition, cleaved αII-spectrin and cleaved caspase-3 levels in the aged spinal cord were markedly increased compared to those in the adult group. These findings suggest that the increases of cleaved αII-spectrin and cleaved caspase-3 immunoreactivity may be related to aging of the spinal cord in dogs.

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“…In both adult and aged groups, we found that the staining pattern and number of NeuN (a marker for neurons) immunoreactive neurons were similar in both groups [27][28][29].…”

Section: Neun Immunoreactivitymentioning

confidence: 78%

Section: Discussionmentioning

confidence: 94%

Section: Experimental Animalsmentioning

confidence: 88%

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Increased Immunoreactivities of Cleaved αII-Spectrin and Cleaved Caspase-3 in the Aged Dog Spinal Cord

et al. 2011

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“…of canine senescence and oncogenesis Ahn et al (2011) found 4-HNE, SOD1 and SOD2 to be present in the cervical and lumbar spinal cords of 10 to 12-year-old but not two to threeyear-old dogs. They reported that the numbers of 4-HNE-, SOD1-and SOD2-immunoreactive cells was significantly higher in both the cervical and lumbar spinal cord of the older dogs and suggested a role for these proteins in generating DNA damage in neurons.…”

Section: The Biochemical and Molecular Basismentioning

confidence: 99%

“…Because of its numerous physiologic and genetic similarities to the human, the domestic dog (Canis familiaris) is an important model for the aging process and neoplasm studies (Ahn et al 2011;. The clinical and biological similarities between naturally occurring human and canine neoplastic processes are difficult to reproduce in other animal models.…”

Section: Theories Of Body Senescence Vs Neoplastic Transformationmentioning

confidence: 99%

The immunological, biochemical and molecular bases of canine senescence and carcinogenesis: a review

Woźna¹,

Kempisty²,

Piotrowska³

et al. 2012

Vet. Med. - Czech

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ABSTRACT:Senescence is a complex set of processes involving several biochemical, molecular and metabolomic changes, including also many disturbances in the immunological system. There are many factors, described as intrinsic and extrinsic (environmental), that may lead to advanced body senescence. In this review, several of the biochemical as well as molecular factors involved in senescence are described. The importance of immunological deficiencies as well as changes in the immunological response after induction of senescence is also highlighted. Furthermore, the molecular basis of canine carcinogenesis in relation to interleukin expression and activation as well as the role of CD leukocyte common antigen in the identification of cancer development and progression, are also described. Keywords: senescence; carcinogenesis; canine List of abbreviations4-HNE = 4-hydroxynonenal, Aβ1-42 = β-amyloid form, AD = Alzheimer disease, alpha(1)AG = alpha 1 acid glycoprotein, bcl-2 = B-cell leukemia 2, BER = base-excision repair, BRCA1 = breast cancer susceptibility gene-1, CD = cluster of differentiation, coating the surface of B lymphocytes and T lymphocytes, CTVT = canine transmissible venereal tumour, FOXP3 = forkhead box P3, scurfin, IFN-gamma = interferon-gamma, IL = interleukin, IVL = intravascular lymphoma, MGT's = mammary gland tumours, MHC = major histocompatibility complex, NFTs = neurofibrillary tangles, NHL = non-Hodgkin's lymphoma, NK = natural killer, OGG1 = 8-oxoguanine glycosylase 1, OSA = osteosarcoma, PAC-1 = first procaspase activating compound, PBLs = peripheral blood lymphocytes, pIL = interleukin plasmids, SLC1A3 = solute carrier family 1 (glial high affinity glutamate transporter), member 3, SOD = superoxide dismutase, TCR = T-cell receptor, TCRBCL = T-cell rich B-cell lymphoma, TGF-beta = transforming growth factor beta, TIL's = tumour-infiltrating lymphocytes, Tregs = T regulatory cells, VEGF = vascular endothelial growth factor

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Early IV-injected human dermis-derived mesenchymal stem cells after transient global cerebral ischemia do not pass through damaged blood-brain barrier

Ahn

Chen

Park

et al. 2018

J Tissue Eng Regen Med

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There is lack of researches on effects of intravenously injected mesenchymal stem cells (MSCs) against transient cerebral ischemia (TCI). We investigated the disruption of the neurovascular unit (NVU), which comprises the blood-brain barrier and examined entry of human dermis-derived MSCs (hDMSCs) into the damaged hippocampal CA1 area in a gerbil model of TCI and their subsequent effects on neuroprotection and cognitive function. Impairments of neurons and blood-brain barrier were examined by immunohistochemistry, electron microscopy, and Evans blue and immunoglobulin G leakage. Neuronal death was observed in pyramidal neurons 5-day postischemia. NVU were structurally damaged; in particular, astrocyte end-feet were severely damaged from 2-day post-TCI and immunoglobulin G leaked out of the CA1 area 2 days after 5 min of TCI; however, Evans blue extravasation was not observed. On the basis of the results of NVU damages, ischemic gerbils received PKH2-transfected hDMSCs 3 times at early times (3 hr, 2, and 5 days) after TCI, and fluorescence imaging was used to detect hDMSCs in the tissue. PKH2-transfected hDMSCs were not found in the CA1 from immediate time to 8 days after injection, although they were detected in the liver. Furthermore, hDMSCs transplantation did not protect CA1 pyramidal neurons and did not improve cognitive impairment. Intravenously transplanted hDMSCs did not migrate to the damaged CA1 area induced by TCI. These findings suggest no neuroprotection and cognitive improvement by intravenous hDMSCs transplantation after 5 min of TCI.

show abstract

Comparison of immunoreactivities in 4-HNE and superoxide dismutases in the cervical and the lumbar spinal cord between adult and aged dogs

Cited by 15 publications

References 40 publications

Increased Immunoreactivities of Cleaved αII-Spectrin and Cleaved Caspase-3 in the Aged Dog Spinal Cord

Increased Immunoreactivities of Cleaved αII-Spectrin and Cleaved Caspase-3 in the Aged Dog Spinal Cord

The immunological, biochemical and molecular bases of canine senescence and carcinogenesis: a review

Early IV-injected human dermis-derived mesenchymal stem cells after transient global cerebral ischemia do not pass through damaged blood-brain barrier

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