Comparison of Immunocytologic Localization of Insulin- Like Growth Factor Binding Protein-4 in Normal and Polycystic Ovary Syndrome Human Ovaries.

Xiang, Peng; Maruo, Takeshi; Samoto, Takashi; Mochizuki, Matsuto

doi:10.1507/endocrj.43.269

Cited by 12 publications

(6 citation statements)

References 40 publications

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“…In human ovaries IGFBP-2 and -4 are known to inhibit the action of IGF-II, which they predominantly bind. IGFBP-2 and -4 can be localized in granulosa and theca cells of atretic follicles, but are negligible in primordial, preantral and antral follicles (29). The follicular fluid of atretic/androgen-dominant follicles contains high levels of IGFBP-2 and -4, subsequently low levels and activity of IGF-II (47)(48)(49).…”

Section: Discussionmentioning

confidence: 99%

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Actions of GnRH antagonists on IGF-II, IGF-binding protein-2 and pregnancy-associated plasma protein-A in human granulosa-lutein cells

Weiss

Krautmacher

Polack

et al. 2003

European Journal of Endocrinology

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Objective: Recently, GnRH antagonists (GnRHants) have been introduced for the prevention of premature LH surges during controlled ovarian hyperstimulation (COH). Here we investigated whether the GnRHants cetrorelix and ganirelix exert effects on the human ovarian IGF system. Since controversy exists on the action of GnRH agonists in the human ovary, we also tested the effect of triptorelin on IGF-II, IGF-binding protein-2 (IGFBP-2) and pregnancy-associated plasma protein-A (PAPP-A) in cultured human granulosa-lutein cells. Design: In vitro cell culture study in a research laboratory of a university hospital. Patients: Cells were obtained from patients treated with different protocols of COH. In addition to gonadotropins they received triptorelin or cetrorelix. Cells were treated with triptorelin, cetrorelix or ganirelix, 1 nmol/l each, for 48 h. IGF-II, IGFBP-2 and PAPP-A were measured by RIA and enzyme immunoassay respectively. Results: GnRHants and triptorelin did not affect IGF-II, IGFBP-2 or PAPP-A. Conclusions: We conclude that GnRHants do not exert any significant effects on the IGF system of granulosa-lutein cells and therefore their introduction into protocols of COH is unlikely to impair ovarian function.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The synthesis of IGF-II in the estrogen-dominant follicle increases dramatically at the time of follicle selection (28). It binds to IGF-binding proteins IGFBP-2 and IGFBP-4 (29). Recent studies noted that in dominant and luteinized follicles levels of IGFBP-4 were low.…”

Section: Introductionmentioning

confidence: 99%

Actions of GnRH antagonists on IGF-II, IGF-binding protein-2 and pregnancy-associated plasma protein-A in human granulosa-lutein cells

Weiss

Krautmacher

Polack

et al. 2003

European Journal of Endocrinology

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“…In addition to this provocative hypothesis, it is also known that IGFBP-4 is found in abundance in patients with PCOS when compared with normo-ovulatory women, being expressed in the granulosa and theca cells. [82][83][84] Taken together, lowered PAPP-A activity and high IGFBP4 concentrations in conditions such as PCOS may result in lower bioavailable IGFs and further lack of synergism of gonadotropins and IGFs. This contribution to an androgenic environment, as observed in PCOS, may also impair ovulation and lead to follicular regression and atresia (Fig.…”

Section: Ovarian Igf Binding Proteins and Proteases: Control Of Bioavmentioning

confidence: 99%

The Interplay of Insulin-Like Growth Factors, Gonadotropins, and Endocrine Disruptors in Ovarian Follicular Development and Function

Kwintkiewicz¹,

Giudice²

2009

Semin Reprod Med

102

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Over the past 20 years, the expression, signaling mechanisms, and roles of members of the insulin-like growth factor (IGF) family (ligands, receptors, binding proteins, and binding protein proteases and their inhibitors) have been elucidated in ovarian follicle function in humans and other species. In vitro studies with human, nonhuman primate, and farm animal granulosa and thecal cells and genetic approaches using mouse knockout models for IGF family members have revealed that IGFs are key intraovarian regulators of follicle growth, selection, atresia, cellular differentiation, and steroidogenesis, oocyte maturation, and cumulus expansion. Some of these actions are synergistic with gonadotropins, although most are not sustainable with IGFs alone and require gonadotropin actions, thereby designating IGFs as "co-gonadotropins." In the human disorder of polycystic ovarian syndrome, characterized by small antral follicle arrest, the IGF system appears to contribute to the observed resistance to follicle-stimulating hormone action at the level of the granulosa compartment and the persistence of an androgen-dominant milieu in the arrested follicles. Interestingly, recent studies demonstrate that endocrine-disrupting chemicals can compromise IGF activity and signaling in the ovarian follicle, affecting follicle development, steroidogenesis, and oocyte quality. The successful development of a healthy oocyte and appropriate granulosa and theca cell steroidogenesis on a cyclic basis are contingent on multiple factors, including a properly functioning intraovarian IGF system. Disruption of even one component of this system can lead to abnormal follicular development and function and compromised reproductive capacity.

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“…In normal human ovaries, IGFBP-4 can be immunolocalized to the granulosa and theca cells of atretic follicles, but is negligible in primordial, preantral and antral follicles (Peng et al 1996). The follicular fluid of atretic/androgendominant follicles contains high levels of IGFBP-2 and IGFBP-4, which are known to inhibit the action of IGF-II (Cataldo & Giudice 1992a, San Roman & Magoffin 1993, Giudice et al 1995.…”

Section: Follicular Growth and Developmentmentioning

confidence: 99%

“…The IGF-Iinduced estradiol production is also modulated by other species of IGFBPs. The abundant expression of IGFBP-4 in granulosa and theca cells of preantral and antral follicles of PCOS ovaries may lead to a decrease in the bioavailability of IGF-I in those follicles (Peng et al 1996). The decrease in IGF-I-mediated stimulation of gonadotropin actions on granulosa and theca cells in preantral and antral follicles may in turn impair the induction of aromatase activity, causing an androgenic microenvironment which is characteristic of atretic follicles and PCOS follicles (Peng et al 1996).…”

Section: Polycystic Ovarian Syndrome (Pcos)mentioning

confidence: 99%

IGFs and IGF-binding proteins in the regulation of human ovarian and endometrial function

Hs¹,

Chard²

1999

Journal of Endocrinology

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Comparison of Immunocytologic Localization of Insulin- Like Growth Factor Binding Protein-4 in Normal and Polycystic Ovary Syndrome Human Ovaries.

Abstract: Abstract.The cytologic localization and cellular levels of insulin-like growth factor binding protein-4

Cited by 12 publications

References 40 publications

Actions of GnRH antagonists on IGF-II, IGF-binding protein-2 and pregnancy-associated plasma protein-A in human granulosa-lutein cells

Actions of GnRH antagonists on IGF-II, IGF-binding protein-2 and pregnancy-associated plasma protein-A in human granulosa-lutein cells

The Interplay of Insulin-Like Growth Factors, Gonadotropins, and Endocrine Disruptors in Ovarian Follicular Development and Function

IGFs and IGF-binding proteins in the regulation of human ovarian and endometrial function

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