1978
DOI: 10.1016/0091-6749(78)90470-0
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Comparison of immune reactivity to polyvalent monomeric and polymeric ragweed antigens

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1978
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Cited by 43 publications
(8 citation statements)
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“…We have demonstrated that mo lecular weight is inversely proportional to allergenici ty [8], Thus the polymers with molecular weight higher than 20,000,000 daltons should be safer (less allergen ic) than lower molecular weight polymers, and we have now included that material in our preparation and eliminated the Sepharose 4B fractionation step. The change from Sephadex G-200 to Sephacryl S-300 allows fractionation of material at any molecular weight limit from 1,000,000 to 240,000 daltons after using various molecular weight markers to calibrate the column.…”
Section: Discussionmentioning
confidence: 99%
“…We have demonstrated that mo lecular weight is inversely proportional to allergenici ty [8], Thus the polymers with molecular weight higher than 20,000,000 daltons should be safer (less allergen ic) than lower molecular weight polymers, and we have now included that material in our preparation and eliminated the Sepharose 4B fractionation step. The change from Sephadex G-200 to Sephacryl S-300 allows fractionation of material at any molecular weight limit from 1,000,000 to 240,000 daltons after using various molecular weight markers to calibrate the column.…”
Section: Discussionmentioning
confidence: 99%
“…This successful result is attribut able to two reasons: the first is that coupling of a heterologous antigenic enzyme with a nonimmunogenic homologous protein prov ides a way to eliminate or suppress the im mune response and the second is that pro tection of the catalytic site with the corre sponding substrate, L-asparagine in this case, serves to keep the enzyme activity in the process of glutaraldehyde treatment. Formaldehyde or glutaraldehyde polymeri zation has been done on a variety of pollen proteins to reduce their allergenic activities [11,14,18,20],…”
Section: Discussionmentioning
confidence: 99%
“…The gluteraldehyde-polymerized ragweed extract (PRW; kindly supplied by Allergy Laboratories of Ohio, Columbus, Ohio, USA) was made from a starting aqueous ragweed monomer extract con taining antigen 200 /rg antigen E (AgE)/mI, using the method de scribed by Patterson et al [7]. Serial 10-fold dilutions were made for treatment solutions.…”
Section: Antigensmentioning
confidence: 99%