Comparison of IgE values as determined by different solid phase radioimmunoassay methods

Johansson, S. G. O.; Berglund, Asta; Kjellman, N.‐I. M.

doi:10.1111/j.1365-2222.1976.tb01417.x

Cited by 82 publications

(19 citation statements)

References 11 publications

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“…Nevertheless, we sus pect that technical difficulties were the main causes of these discrepant results. The high IgE values previ ously found with RIST in some fluids, such as CSF, AH and tears, and the lack of correlation with serum IgE should have raised suspicion since these results suggested a local IgE production even in normal sub jects, all the more so as there were early suspicions that RIST gave falsely high values in urine and saliva [Stokes et al, 1973], and later in serum when low con centrations of IgE were to be assayed [Polmar et al, 1973;Johansson et al, 1976].…”

Section: Discussionmentioning

confidence: 99%

A Reappraisal of IgE Levels in Various Human Secretions by Particle Counting Immunoassay Combined with Pepsin Digestion

Magnusson¹,

Masson²

1985

Int Arch Allergy Immunol

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We have assayed IgE in various human secretions by particle-counting immunoassay (PACIA). The introduction of a pepsin digestion step of the sample prior to the assay not only eliminates possible interfering factors but also releases from IgE the protease-resistant FcXXX_ε fragment. Since this fragment could represent the residual form of IgE in certain secretions rich in proteases it should be a suitable calibrator for the assay of IgE in secretions. The concentrations of IgE that we found in most fluids (urine, saliva, tears, milk, aqueous humour, cerebrospinal fluid, bronchoalveolar washings, intestinal fluids and faecal extracts) were up to 100 times lower than those reported by others who have used the radioimmunosorbent test (RIST). We suspect that the high values found with RIST are related to the susceptibility of this test to various interfering factors and not to a local production of IgE in most secretions of normal individuals.

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Section: Discussionmentioning

confidence: 99%

A Reappraisal of IgE Levels in Various Human Secretions by Particle Counting Immunoassay Combined with Pepsin Digestion

Magnusson¹,

Masson²

1985

Int Arch Allergy Immunol

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“…5) Recent allergen exposure. 6) The method used; low IgE levels being more accurately determined by the PRIST than by the RIST method (20).…”

Section: Ige Determnationmentioning

confidence: 99%

Clinical Investigation of Allergic Rhinitis and Allied Conditions

Mygind

1979

Allergy

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“…All infants were born in our hospital and 95% of them came from a flemish suburban populations. The radioimmunological Phadebas IgE Prist dosage method (Pharmacia Diagnostics AB, Uppsala, Sweden) was used [11]. The test was callibrated for a detection limit of 0.1 U/ml.…”

Section: Methodsmentioning

confidence: 99%

Influences of neonatal serum IgE concentration, family history and diet on the incidence of cow's milk allergy

Vandenplas

Sacré

1986

Eur J Pediatr

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Serum IgE concentration was measured on the 5th day of life in 943 infants. All infants were included in a 3 month follow-up study. The frequency of cow's milk allergy was studied according to either family history, IgE level, or both. Feeding (mother's milk or formula feeding) was taken into account. Manifestations suggestive of food allergy were hardly observed in breast-fed babies. In the formula-fed group a positive family history correlated with a 40% incidence of allergic manifestations, compared to a 13% incidence (P less than 0.001) in the group with negative family history. A high IgE level (IgE greater than 1.3 U/ml) indicated a 43% risk of developing allergic manifestations in formula-fed babies as compared to 15% (P less than 0.001) in the group with normal results of a screening test. Frequency of allergic manifestations in a subgroup with a negative family history and a high IgE level (38%) was equal to the frequency in the subgroup with a positive family history and negative screening test results (IgE less than 1.3 U/ml)(36%). The incidence in the subgroup with both positive screening test results and a positive family history was 49%. None of these differences were significant. The frequency in the subgroup with both parameters negative was 8% (P less than 0.001 to 3 other subgroups). Our results indicate that the family history seems to correlate as well with the incidence of allergic manifestations as the neonatal serum IgE concentration.(ABSTRACT TRUNCATED AT 250 WORDS)

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Comparison of IgE values as determined by different solid phase radioimmunoassay methods

Cited by 82 publications

References 11 publications

A Reappraisal of IgE Levels in Various Human Secretions by Particle Counting Immunoassay Combined with Pepsin Digestion

A Reappraisal of IgE Levels in Various Human Secretions by Particle Counting Immunoassay Combined with Pepsin Digestion

Clinical Investigation of Allergic Rhinitis and Allied Conditions

Influences of neonatal serum IgE concentration, family history and diet on the incidence of cow's milk allergy

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