Comparison of <i>in vivo</i> and <i>in vitro</i> digestion on polyphenol composition in lingonberries: Potential impact on colonic health

Brown, Emma M.; Nitecki, S.; Pereira-Caro, Gema; McDougall, Gordon J.; Stewart, Derek; Rowland, Ian; Crozier, Alan; Gill, Chris

doi:10.1002/biof.1173

Cited by 60 publications

(31 citation statements)

References 33 publications

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“…We noted earlier, that many small phenolic molecules can also be generated by GMB-mediated degradation of polyphenol rich dietary components [82][83][84][85][86][87][88][89]. This applies to all 3 phenolic metabolites in our study (phenol sulfate, p-cresol sulfate, HA).…”

Section: Associations With Specific Gut Microbiotasupporting

confidence: 67%

“…On the other hand mixed fecal cultures or certain constituents of the human GMB can, under anaerobic conditions, generate BA or its hydroxylated metabolites from a variety of precursors, including free L-tyrosine or L-phenylalanine [80,81], 4-hydroxyphenylpyruvic acid [81], polyphenol rich dietary components (eg, berries, black tea, bran, fruit juices, wine) [82][83][84][85][86][87][88][89], or pure polyphenols themselves (eg, catechin, epicatechin, proanthocyanidins, quercetin) [90][91][92][93].…”

Section: Phenolic Metabolitesmentioning

confidence: 99%

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Changes in the Serum Metabolome of Patients Treated With Broad-Spectrum Antibiotics

Jaskiw

Obrenovich

Kundrapu

et al. 2020

PAI

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Background: The gut microbiome (GMB) generates numerous small chemicals that can be absorbed by the host and variously biotransformed, incorporated, or excreted. The resulting metabolome can provide information about the state of the GMB, of the host, and of their relationship. Exploiting this information in the service of biomarker development is contingent on knowing the GMB-sensitivity of the individual chemicals comprising the metabolome. In this regard, human studies have lagged far behind animal studies. Accordingly, we tested the hypothesis that serum levels of chemicals unequivocally demonstrated to be GMB-sensitive in rodent models would also be affected in a clinical patient sample treated with broad spectrum antibiotics.Methods: We collected serum samples from 20 hospitalized patients before, during, and after treatment with broad-spectrum antibiotics. We also collected samples from 5 control patients admitted to the hospital but not prescribed antibiotics. We submitted the samples for a non-targeted metabolomic analysis and then focused on chemicals known to be affected both by germ-free status and by antibiotic treatment in the mouse and/or rat.Results: Putative identification was obtained for 499 chemicals in human serum. An aggregate analysis did not show any time x treatment interactions. However, our literature search identified 10 serum chemicals affected both by germ-free status and antibiotic treatment in the mouse or rat. Six of those chemicals were measured in our patient samples and additionally met criteria for inclusion in a focused analysis. Serum levels of 5 chemicals (p-cresol sulfate, phenol sulfate, hippurate, indole propionate, and indoxyl sulfate) declined significantly in our group of antibiotic-treated patients but did not change in our patient control group.Conclusions: Broad-spectrum antibiotic treatment in patients lowered serum levels of selected chemicals previously demonstrated to be GMB-sensitive in rodent models. Interestingly, all those chemicals are known to be uremic solutes that can be derived from aromatic amino acids (L-phenylalanine, L-tyrosine, or L-tryptophan) by anaerobic bacteria, particularly Clostridial species. We conclude that judiciously selected serum chemicals can reliably detect antibiotic-induced suppression of the GMB in man and thus facilitate further metabolome-based biomarker development.

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Section: Associations With Specific Gut Microbiotasupporting

confidence: 67%

Section: Phenolic Metabolitesmentioning

confidence: 99%

Changes in the Serum Metabolome of Patients Treated With Broad-Spectrum Antibiotics

Jaskiw

Obrenovich

Kundrapu

et al. 2020

PAI

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“…More in vitro digestion studies would be useful to understand the stability of seaweed polyphenols as extracts or within the seaweed matrix [155,156]. These studies may be complemented by studies which use ileostomy patient cohorts to determine structural changes to seaweed polyphenols following upper GI digestion in vivo to indicate polyphenol bioaccessibility in the colon [157].…”

Section: Polyphenolsmentioning

confidence: 99%

Prebiotics from Seaweeds: An Ocean of Opportunity?

et al. 2019

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Seaweeds are an underexploited and potentially sustainable crop which offer a rich source of bioactive compounds, including novel complex polysaccharides, polyphenols, fatty acids, and carotenoids. The purported efficacies of these phytochemicals have led to potential functional food and nutraceutical applications which aim to protect against cardiometabolic and inflammatory risk factors associated with non-communicable diseases, such as obesity, type 2 diabetes, metabolic syndrome, cardiovascular disease, inflammatory bowel disease, and some cancers. Concurrent understanding that perturbations of gut microbial composition and metabolic function manifest throughout health and disease has led to dietary strategies, such as prebiotics, which exploit the diet-host-microbe paradigm to modulate the gut microbiota, such that host health is maintained or improved. The prebiotic definition was recently updated to “a substrate that is selectively utilised by host microorganisms conferring a health benefit”, which, given that previous discussion regarding seaweed prebiotics has focused upon saccharolytic fermentation, an opportunity is presented to explore how non-complex polysaccharide components from seaweeds may be metabolised by host microbial populations to benefit host health. Thus, this review provides an innovative approach to consider how the gut microbiota may utilise seaweed phytochemicals, such as polyphenols, polyunsaturated fatty acids, and carotenoids, and provides an updated discussion regarding the catabolism of seaweed-derived complex polysaccharides with potential prebiotic activity. Additional in vitro screening studies and in vivo animal studies are needed to identify potential prebiotics from seaweeds, alongside untargeted metabolomics to decipher microbial-derived metabolites from seaweeds. Furthermore, controlled human intervention studies with health-related end points to elucidate prebiotic efficacy are required.

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“…26 The United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Horticultural Crops Research Unit (HCRU) recommended to standardize Vaccinium vitis-idaea L. fruits by the amounts of the three anthocyanes described above because there are already several cases of adulteration of these valuable plant materials. 32 Brown et al 33 2).…”

Section: Anthocyanesmentioning

confidence: 99%

Vaccinium vitis-idaea L.: Chemical Contents, Pharmacological Activities

et al. 2020

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One of the most known species of the genus Vaccinium (Ericaceae) is Vaccinium vitis-idaea L. or lingonberry. Leaves are included in the State Pharmacopoeia of the Russian Federation (XIV-th edition) and the State Pharmacopoeia of the Republic of Belarus (II-nd edition). The aim of this review is an analysis of data about a chemical content and types of pharmacological activities of Vaccinium vitis-idaea L. to discuss the tendency of future investigations on this plant. The main parts of works describe researches of chemical contents of fruits as medicinal and edible plant material. The majority of researches describe results of in vitro experiments. A significant interest is the study of the neuroprotective activity of the Vaccinium vitis-idaea extracts as well as their anti-cytokine and antiapoptotic properties and metabolic effects. The main biologically active compounds are phenologlycosides, tannins, proanthocyanes, saponins etc. These results will be of great significance for the development of new drugs from this plant and use along with the fruits of other parts of the plant.

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Comparison of in vivo and in vitro digestion on polyphenol composition in lingonberries: Potential impact on colonic health

Cited by 60 publications

References 33 publications

Changes in the Serum Metabolome of Patients Treated With Broad-Spectrum Antibiotics

Changes in the Serum Metabolome of Patients Treated With Broad-Spectrum Antibiotics

Prebiotics from Seaweeds: An Ocean of Opportunity?

Vaccinium vitis-idaea L.: Chemical Contents, Pharmacological Activities

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