Comparison of<i>in vitro</i>-cultivation of human mesenchymal stroma/stem cells derived from bone marrow and umbilical cord

Hoffmann, Andrea; Floerkemeier, Thilo; Melzer, Catharina; Hass, Ralf

doi:10.1002/term.2153

Cited by 35 publications

(28 citation statements)

References 143 publications

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“…Mesenchymal stem/stromal cells (MSC) represent an important group of adult stem cells with multidifferentiation potential and immunomodulatory properties, making them an important cell type for cell therapy purposes . Currently, more than 400 studies exploring the therapeutic effects of MSC have been registered on the clinical trial database (http://www.clinicaltrials.gov) to treat diseases such as GvHD, organ transplantation, diabetes, multiple sclerosis, and Crohn .…”

Section: Introductionmentioning

confidence: 99%

Expansion strategies for human mesenchymal stromal cells culture under xeno‐free conditions

Tozetti

Caruso

Mizukami

et al. 2017

Biotechnology Progress

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Choosing the culture system and culture medium used to produce cells are key steps toward a safe, scalable, and cost-effective expansion bioprocess for cell therapy purposes. The use of AB human serum (AB HS) as an alternative xeno-free supplement for mesenchymal stromal cells (MSC) cultivation has increasingly gained relevance due to safety and efficiency aspects. Here we have evaluated different scalable culture systems to produce a meaningful number of umbilical cord matrix-derived MSC (UCM MSC) using AB HS for culture medium supplementation during expansion and cryopreservation to enable a xeno-free bioprocess. UCM MSC were cultured in a scalable planar (compact 10-layer flasks and roller bottles) and 3-D microcarrier-based culture systems (spinner flasks and stirred tank bioreactor). Ten layer flasks and roller bottles enabled the production of 2.6 ± 0.6 × 10 and 1.4 ± 0.3 × 10 cells/cm . UCM MSC-based microcarrier expansion in the stirred conditions has enabled the production of higher cell densities (5.5-23.0 × 10 cells/cm ) when compared to planar systems. Nevertheless, due to the moderate harvesting efficiency attained, (80% for spinner flasks and 46.6% for bioreactor) the total cell number recovered was lower than expected. Cells maintained the functional properties after expansion in all the culture systems evaluated. The cryopreservation of cells (using AB HS) was also successfully carried out. Establishing scalable xeno-free expansion processes represents an important step toward a GMP compliant large-scale production platform for MSC-based clinical applications. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1358-1367, 2017.

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Section: Introductionmentioning

confidence: 99%

Expansion strategies for human mesenchymal stromal cells culture under xeno‐free conditions

Tozetti

Caruso

Mizukami

et al. 2017

Biotechnology Progress

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“…The isolation of MSCs derived from the bone marrow is quite simple because it uses the adhesion of these cells to the plastics. Thanks to this, other cells can be separated by rinsing them out several times [8]. MSC can also be obtained from Wharton jelly after thorough cleansing from the blood.…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

“…The resulting solution is centrifuged at 300 g for 5 minutes and the pellet can be used for cell culture. The whole procedure must be sterile to avoid the possibility of infection [8,9].…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

Application potential and plasticity of human stem cells

Lorenz

Mozdziak

Kempisty

et al. 2019

Medical Journal of Cell Biology

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Significant advances have been achieved in the study of stem cells over recent years. Stem cell isolation, their plasticity, differentiation and pre-clinical and clinical applications have undergone a significant study. The objective of this paper is to review the advances in stem cell isolation methods. There are many types of stem cells in the article. Isolation and subsequent differentiation of among others: Human adipose-derived stem cells, cancer stem cells, neural stem cells and mesenchymal stem cells. The subject of Endometrial mesenchymal stromal cells, whose isolation methods are relatively new, was also raised. Attention was paid to the development of preclinical studies using Dental Pulp Stem Cells in various diseases such as Parkinson’s disease or Alzheimer’s disease. Progress in research on the use of stem cells in the treatment of heart attacks, burns, bone injuries and the use of neural stem cells in animal models as an attempt to treat multiple sclerosis has been described.Running title: Potential and plasticity of stem cells

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“…Umbilical cord-derived stem cells (UC-MSCs) are usually cultured at 37°C in a humified 5% CO 2 atmosphere. However it has been reported that the atmospheric concentration of the oxygen can be relatively toxic for the cells and the hypoxic conditions could be beneficial for their growth [5,22]. Since UC-MSCs are plastic-adherent, they are cultured either in flasks or plates and the culture medium is most commonly supplemented with 10% fetal bovine serum (FBS), however the use of 15% FBS has been described as well [23,24].…”

Section: In Vitro Cultivation Of Umbilical Cordderived Cellsmentioning

confidence: 99%

Overview of methods of isolation, cultivation and genetic profiling on human umbilical cord stem cells

Stefańska

Sibiak

Dompe

et al. 2019

Medical Journal of Cell Biology

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Stem cells possess unique properties, such as self-renewal ability or differentiation capacity into more specialized cells, which makes them particularly relevant for regenerative medicine and cellular therapies. Although embryonic stem cells (ESCs) are capable of differentiation into all cell lineages, their utilization is associated with ethical concerns since they are obtained from embryos. Furthermore, ESCs may form teratomas or cause immune rejection in the clinical setting. Therefore, an effort has been made to utilize stem cells derived from adult tissues, especially mesenchymal stem cells (MSCs). A particularly attractive source of MSCs is the human umbilical cord, which is typically discarded after birth and considered a medical waste, therefore the acquisition of the cells is not associated with any health risk for a patient. Moreover, umbilical cord-derived MSCs do not express MHCII, thus they exhibit reduced immunogenicity. MSCs have been isolated from all compartments of umbilical cord, however the Wharton's jelly-derived MSCs (WJ-M-SCs) are the most clinically utilizable. There are two techniques of UC-MSCs isolation: the enzymatic and explant procedures. The explant method involves cell outgrowth of tissue pieces placed into plastic culture vessel after mechanical splitting, whereas the enzymatic technique involves minced tissue digestion in an enzymatic solution. In vitro culture conditions of the isolated cells are highly variable among the researchers, however the most commonly performed molecular assays are homogenous and include: RT-qPCR, flow cytometry and immunocytochemistry.Running title: Human umbilical cord stem cells isolation, cultivation and genetic profiling

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Comparison ofin vitro-cultivation of human mesenchymal stroma/stem cells derived from bone marrow and umbilical cord

Cited by 35 publications

References 143 publications

Expansion strategies for human mesenchymal stromal cells culture under xeno‐free conditions

Expansion strategies for human mesenchymal stromal cells culture under xeno‐free conditions

Application potential and plasticity of human stem cells

Overview of methods of isolation, cultivation and genetic profiling on human umbilical cord stem cells

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