Comparison of Crinipellis perniciosa isolates from Brazil by ERIC repetitive element sequence‐based PCR genomic fingerprinting

Abstract

Fifty isolates of Crinipellis perniciosa originating from Theobroma cacao , Heteropterys acutifolia and Solanum lycocarpum , from six states within Brazil, were characterized through ERIC-PCR, representing the first application of this method for molecular characterization within C. perniciosa . Phenetic analysis of banding patterns revealed a separation of isolates on the basis of host of origin, with T. cacao -derived isolates showing only a 0·2 similarity level to a cluster comprising the isolates from H. acutifolia and S. lycocarpum . Considerable intraspecific variability was observed within C. perniciosa isolates from T. cacao , with distinct groups observed correlating with geographical origin. Given that a number of isolates from T. cacao from the Amazon region grouped with isolates from Bahia state, this work discusses the possibility that current C. perniciosa populations pathogenic on T. cacao in Bahia originated from the Amazon region, rather than from alternative host plants.

The distribution of a transposase sequence in Moniliophthora perniciosa confirms the occurrence of two genotypes in Bahia, Brazil

“…Thus, it was possible to group the isolates from C-biotype from the Bahia state with the isolates from the Brazilian Amazon region. Arruda et al (2003b) also showed that some isolates of M. perniciosa from the Bahia state are genetically related to isolates from Belém-PA, Brazilian Amazon region. In a parallel study, Arruda et al (2003a) has shown that IGS sequences of isolates from southern Bahia are highly similar to sequences of isolates from the Amazon region.…”

“…At the molecular level, the intraspecific variability of M. perniciosa was demonstrated by the restriction fragment length polymorphism (RFLP) pattern and sequencing of the intergenic spacer (IGS) region from the rDNA (Arruda et al, 2003a). Characterization by Enterobacterial Repetitive Intergenic Consensus (ERIC) repetitive element sequence-based PCR from different isolates of M. perniciosa has also allowed the detection of intraspecific variability and the correlation of this variation with the host and geographic origin of isolates (Arruda et al, 2003b). Rincones et al (2003Rincones et al ( , 2006 showed genotypic polymorphisms among different biotypes and among C-biotype isolates from different regions of southern Bahia by electrophoretic karyotype and microsatellite analysis.…”

Genetic diversity of polysporic isolates of Moniliophthora perniciosa (Tricholomataceae)

“…A.H. Gentry (Bignoniaceae) (Evans, 1978;Hedger et al, 1987;Griffith and Hedger, 1994a,b;Arruda et al, 2003).…”

“…In the Malpighiaceae family, H. acutifolia, a common species in the riparian forests in Minas Gerais State, is also a M. perniciosa host (Resende et al, 2000). In 1985, the occurrence of M. perniciosa in Bixa orellana, which belongs to the Bixaceae family, was documented in Pará State (Bastos and Andebrhan, 1986;Arruda et al, 2003).…”

“…The variability characterization of M. perniciosa has been based on morphologic and cultural characteristics, somatic compatibility (SCG), molecular analysis, and biochemistry relationships with Theobroma cocoa and other Theobroma spp (Hedger et al, 1987;McGeary and Wheeler, 1988;Wheeler and Mepsted, 1988;Griffith and Hedger, 1994a;Arruda et al, 2003;Lana et al, 2011). Anastomosis between mycelium from different origins might give rise to a heterokaryon appearance in the saprophytic mycelium, and variability subsequently increases in the next basidiospore generation.…”