Comparison of HPLC and multivariate regression methods for hydrocortisone and lidocaine analysis of pharmaceutical preparations

Abstract

A reverse-phase high-performance liquid chromatographic (HPLC) method to determine hydrocortisone acetate, hydrocortisone hemisuccinate and lidocaine is described in this paper. The separation was made in a LichrCART C(18) column using a methanol-NaH(2)PO(4)/Na(2)HPO(4) (0.1 mol L(-1)) (pH=4.5) buffer solution as a mobile phase in isocratic mode (60:40 (v/v)). The mobile phase flow rate and the sample volume injected were 1 mL min(-1) and 20 micro L, respectively. The detection was made with a diode-array detector measuring at the maximum for each compound. Quantification limits ranging from 0.18 to 0.84 micro g L(-1) were obtained when the peak area was measured. The method was applied in pharmaceutical formulations that were compared with those obtained by through multivariate regression spectrophotometry and micellar capillary electrophoresis (MEKC). HPLC results are in accordance with the results obtained by MEKC. The spectrophotometric method was suitable only for synthetic samples.

Recent Advances in the Analysis of Steroid Hormones and Related Drugs

“…The performance of various multivariate chemometric and derivative spectrophotometric methods is compared for examples of formulations containing hydrocortisone and ZnBacitracin 31 as well as dexamethasone and polymyxin B. 32 An interesting feature of another method for the assay of spironolactone and hydrochlorothiazide, based on ratio spectrum derivative spectroscopy, making use of a rapidscanning diode-array spectrophotometer, is that it is adopted to flow-injection analysis. 33 The importance of spectrophotometric/colorimetric methods based on chemical reactions has dramatically decreased in the last years both in the analysis of bulk steroid drugs and formulations made thereof.…”

“…Allylestrenol-α-tocopherol tablets, 102 beclomethasone dipropionate inhalers 103 and other formulations (St), 104 betamethasone, its acetate and other corticosteroids in ointments, 105,106 betamethasone and dexamethasone (delicate separation problem! ), 107,108 betamethasone-diclofenac sodium-cyanocobalamine tablet, 109 ethinylestradiol-norgestrel tablet, 114 ethinylestradiollevonorgestrel and ethinylestradiol-gestodene tablets, 117 finasteride tablet (St), 118 fluocortolone pivalate and hexanoate suppositories, 119 hydrocortisone-oxytetracycline-nystatin in pharmaceutical preparations, 120 hydrocortisone acetate and hemisuccinate-lidocaine in pharmaceutical preparations, 121 hydrocortisone acetate-methyl-and propylparaben topical cream (St), 122 hydrocortisone sodium phosphate gel (St), 123 nestorone implants (St), 124 pancuronium bromide injection (St), 125 prednisolone acetate-tetrahydrozoline hydrochlorideofloxacin ophthalmic preparations, 126 prednisolone sodium phosphate implantable infusion pump (St), 127 progesterone in micellar systems and other formulations, 128 triamcinolone, its acetate and acetonide and several other corticosteroids (St), 129 triamcinolone acetonide dermatological patches, 130 triamcinolone acetonide, methyl-and propylparaben topical cream (St). 131 Carefully validated stability-indicating HPLC methods were used for the determination of impurities and degradants in bulk pipecuronium bromide, 132 budesonide, 133 aqueous spironolactone-β-cyclodextrin solutions, 134 and for the photodegradation kinetic study of danazol.…”

Novel HPLC Analysis of Hydrocortisone in Conventional and Controlled-Release Pharmaceutical Preparations

“…However, modern chromatographic techniques such as high-performance liquid chromatography (HPLC) [8–12] and ultra-performance liquid chromatography (UPLC) [13] are preferentially employed for the determination of hydrocortisone in conventional pharmaceutical products such as tablets, creams, and injections. The different HPLC methods employ different chromatographic conditions including the use of complex and sometimes expensive solvent systems.…”

Validation of a Thin-Layer Chromatography for the Determination of Hydrocortisone Acetate and Lidocaine in a Pharmaceutical Preparation

“…Out of various analytical methods, the multivariate regression spectrophotometry, micellar capillary electrophoresis (MEKC), and also RP-HPLC coupled with electrochemical detector to quantitation of these substances in combined dosage forms were applied. However, all of those studies have focused almost on the hydrocortisone acetate and lidocaine hydrochloride analysis in form of suppositories and ointments [15–17]. Therefore, attempts were made in this work to develop a fast, sensitive, robust, and cost-effective TLC-densitometric method suitable for quantitative analysis of hydrocortisone acetate and lidocaine hydrochloride in the commercial (injection) dosage form.…”