Comparison of Histopathology to the Direct Immunofluorescent Antibody Test for the Diagnosis of Infectious Laryngotracheitis in Chickens

Goodwin, Mark; Smeltzer, Martin A.; Brown, John M.; Resurreccion, Reynaldo S.; Dickson, T. G.

doi:10.2307/1591195

Cited by 10 publications

(7 citation statements)

References 8 publications

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“…However, the PCR method is more sensitive and to reduces false positives [17] . On the other hand, FA test was found to be as reliable as histopathological methods [18] . However, it needs for fluorescence microscopy has been reported as a negative aspect [9] .…”

Section: Discussionmentioning

confidence: 95%

Enfeksiyöz Laringotraheitis İle Doğal Enfekte Yumurta Tavuklarında Solunum Kanalı Lezyonları Üzerine İmmunohistokimyasal Çalışmalar

Yavuz¹,

Özdemir²,

Aras³

et al. 2017

Kafkas Univ Vet Fak Derg

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In this study, naturally infected by Gallid Herpesvirus type-1 in laying hens to be diagnosed by pathological and PCR methods. Sixty pieces of hens were collected in coops from Central Anatolia region. After necropsy, routine pathological processes were applied to the trachea/ larynx, sinuses, lungs and air sacs. All organs were also stained by immunoperoxidase method, and PCR methods were applied to formalin fixed paraffin embedded (FFPE) tissues. Immunohistochemically, the positivities were seen in trachea/larynx (78.3%), sinuses (61.6%), lungs (45%) and air sacs (50%). Positive reactions were observed, in mucous and gland epithelia especially located at intracytoplasmic and rarely intranuclear. PCR positivity was observed in the trachea/larynx in 15 (25%) cases, in infraorbital sinus in 11 (18.3%) cases, in lungs in 8 (13.3%) cases and in air sacs in 6 (10%) cases following the tests performed. Following these results, it is easily concluded that histopathology and immunoperoxidase method can usable for diagnosing of the ILT. However, PCR results made by FFPE tissues showed that this method is not adequate to diagnose the ILT alone. Keywords: Histopathology, ILT, Immunohistochemistry, Laying hens, PCR Enfeksiyöz Laringotraheitis İle Doğal Enfekte Yumurta Tavuklarında Solunum Kanalı Lezyonları Üzerine İmmunohistokimyasal Çalışmalar ÖzBu çalışmada Gallid Herpesvirus tip-1 ile doğal enfekte yumurta tavuklarında patolojik yöntemlerle teşhis konularak immunohistokimyasal ve PCR yöntemlerinin teşhiste kullanılabilirliği araştırıldı. Bu amaçla Orta Anadolu'da bulunan bazı illerdeki kümeslerden toplam 60 adet enfekte tavuk toplandı. Yapılan nekropsilerin sonrasında trake, larinks, infraorbital sinus, akciğer ve hava kesesi parçaları alınarak rutin patolojik işlemler uygulandı. Alınan tüm organlar ayrıca indirekt immunperoksidaz yöntemi ile boyandı ve organlara ait formolle fikse edilmiş parafine gömülü (FFPG) dokulara PZR testi yapıldı. İmmunohistokimyasal boyamalar sonucu trake/larinkste %78.3, sinuslarda %61.6, akciğerlerde %45 ve hava keselerinde %50 oranında pozitiflik gözlendi. Pozitif boyanmalar özellikle mukoza ve bez epitellerinde intrasitoplazmik nadiren de intranüklear olarak gözlendi. Aynı zamanda lümene dökülmüş eksudattaki hücre ve sinsityal dev hücrelerinin sitoplazmalarında da boyanmalar tipikti. Yapılan PCR testlerinin ardından trake/larinkste %25, infraorbital sinuslarda %18.3, akciğerlerde %13.3, hava keselerinde %10 oranında pozitiflik gözlendi. Bu sonuçların ardından hastalığın teşhisinde histopatolojik ve immunperoksidaz yönteminin rahatlıkla kullanılabileceği ortaya konmuştur. Ancak, FFPG dokulardan yapılan PCR yönteminin tek başına İLT'yi teşhis etmek için yeterli olmadığı gösterildi.

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Section: Discussionmentioning

confidence: 95%

Enfeksiyöz Laringotraheitis İle Doğal Enfekte Yumurta Tavuklarında Solunum Kanalı Lezyonları Üzerine İmmunohistokimyasal Çalışmalar

Yavuz¹,

Özdemir²,

Aras³

et al. 2017

Kafkas Univ Vet Fak Derg

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“…Additionally, in the present study a few cases of ILT were noted, occasionally in layers, but some authors emphasize that these cases are more frequent (Chacón et al 2007); however, in the cited paper a definitive diagnosis was based on PCR or ELISA. There are some reports from the literature which indicate a highly comparable specific between the histopathology and PCR or immunofluorescence test in the diagnosis of ILT (Goodwin et al 1991, Humberd et al 2002. For many years traditional virus isolation has been considered a gold standard for ILT diagnosis; however, it is a long-term and expensive method for routine diagnostics.…”

Section: Discussionmentioning

confidence: 99%

Histopathology in diagnosis of broiler chicken and layer diseases – review of cases 1999-2010

Dolka¹,

Sapierzyński²,

Bielecki³

et al. 2012

Polish Journal of Veterinary Sciences

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The aim of this study was to estimate the prevalence of histopathological lesions in the different organs in relation to the commercial-type and the age of birds (i.e. broiler chickens and layers). During the period 1999-2010 a total of 189 cases was submitted to the Division of Animal Pathomorphology, Department of Pathology and Veterinary Diagnostics at WULS. Most cases were found in broiler chickens (66.7%). The majority of the histopathological lesions were detected in the liver and lymphoid organs. In of 29% cases of hepatic injury pathognomonic lesions associated with inclusion body hepatitis (IBH) were found. The mean age of birds was 23 days. Among IBH cases proventriculitis (58%) was more often found than gizzard lesions (25.8%). Interestingly, we noted some intranuclear inclusions in the epithelial cells within the proventriculus. A low percentage of histopathological evidence of infectious bursal disease (IBD) was reported in chickens. The gastrointestinal tract was the second most frequent predilection site for histopathological lesions. Histopathological findings within the heart and lungs were less common and were more often seen in the upper respiratory tract. Cases of infectious laryngotracheitis (ILT) were registered in broiler chickens (3.2%, mean age 37 days) and in layers (4.8%; mean age 196 days). Lesions associated with Marek's disease, avian leukosis and fowl pox were recognized only in layers, respectively in 3.2% (mean age 176 days), 1.6% (mean age 205 days) and 1.1% (mean age 196 days) of all cases. Avian encephalomyelitis (AE) was noted only in 0.5% of all cases.

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“…ILTV strains are antigenically homogenous, naturally vary in virulence, from highly virulent strains, causing high morbidity and mortality and low virulence that produce mild-to-unapparent infection (Bauer et al, 1999). Chickens are infected for ILTV through the upper respiratory and ocular routes (Goodwin et al, 1991). Clinical sings include gasping, depression, nasal discharge, conjunctivitis and expectoration of body mucus.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence study of infectious laryngotracheitis virus antibody of commercial layer in Gazipur Districts of Bangladesh

Uddin

Hassan

et al. 2018

Asian J. Med. Biol. Res

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This research work was conducted to detect the prevalence of Infectious Laryngotracheitis (ILT) Virus-specific antibody in chickens from Gazipur district in Bangladesh at Department of Microbiology, Hajee Mohammad Danesh Science & Technology University, Dinajpur and Poultry Care Lab, Garzipur from January to June 2012. A total number of 232 sera sample of commercial layer chicken were collected from 17 different commercial layer farms at different ages. The layers prognosed for sampling had not been previously vaccinated against ILTV. The indirect enzyme linked immunosorbent assay (iELISA) was performed to estimate the Infectious Laryngotracheitis (ILT) Virus-specific antibody. Out of 232 samples, 189 (81.47%) samples were found to positive for Infectious Laryngotracheitis (ILT) Virus-specific antibody. In 17 different commercial farms prevalence based on age were 75%, 87.5%, 87.5%, 90%, 81.25%, 80%, 100%, 70%, 81.25%, 81.25%, 90%, 93.08%, 87.5%, 75%, 75%, 68.75% and 75% in the age limit 08, 09, 11, 12, 13, 14, 15, 16, 17, 17, 19, 21, 25, 31, 35, 44 and 51 weeks respectively and farms showed high level of ILT virus specific antibodies (IgG). This result showed that in 15 weeks of age prevalence was highest position i.e; 100%. The result of this study indicate that there were a high prevalence of Infectious Laryngotracheitis (ILT) Virus circulating at Gazipur district in Bangladesh.Asian J. Med. Biol. Res. March 2018, 4(1): 1-6

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Comparison of Histopathology to the Direct Immunofluorescent Antibody Test for the Diagnosis of Infectious Laryngotracheitis in Chickens

Cited by 10 publications

References 8 publications

Enfeksiyöz Laringotraheitis İle Doğal Enfekte Yumurta Tavuklarında Solunum Kanalı Lezyonları Üzerine İmmunohistokimyasal Çalışmalar

Enfeksiyöz Laringotraheitis İle Doğal Enfekte Yumurta Tavuklarında Solunum Kanalı Lezyonları Üzerine İmmunohistokimyasal Çalışmalar

Histopathology in diagnosis of broiler chicken and layer diseases – review of cases 1999-2010

Seroprevalence study of infectious laryngotracheitis virus antibody of commercial layer in Gazipur Districts of Bangladesh

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