Comparison of hepatitis C virus serotyping and genotyping in French patients

Castelain, Sandrine; Zawadzki, Patricia; Khorsi, Hafida; Darchis, J P; Capron, Dominique; Sueur, Jean-Marie; Eb, F.; Duverlie, Gilles

doi:10.1016/s0928-0197(96)00266-8

Cited by 7 publications

(5 citation statements)

References 16 publications

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“…Therefore, all these results confirm the ' corrected ' prevalence of HCV-4 in our area to be at least 8n7 %, i.e. twice as high as the previously reported prevalence of HCV-4 observed among the French population (4n5 and 4n1 %) (Castelain et al, 1997 ;Martinot-Peignoux et al, 1999). The characterization of various HCV-4 subtypes and the high prevalence of HCV-4 among patients in our area might be explained (i) by immigration of patients who were most probably infected with HCV-4 in Africa and (ii) by the emergence of two distinct HCV-4 subclades in France among IVDUs.…”

Section: Discussionsupporting

confidence: 91%

“…Since January 1996, anti-HCV-positive serum samples from patients from the Seine-Saint-Denis district of north-east Paris, France, have been prospectively tested against viral peptides that represent the six major HCV genotypes (HCV serotyping 1-6 assay HCO2, Murex) (Leruez-Ville et al, 1998). A high prevalence of HCV-4 infection was observed (10n2 %), which is in contrast to what has been described previously in the French population (Castelain et al, 1997 ;Martinot-Peignoux et al, 1999). To confirm such a high prevalence and to further investigate HCV lineages, we combined phylogenetic in-ference methods with epidemiological information.…”

Section: Introductionmentioning

confidence: 92%

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Phylogenetic analyses confirm the high prevalence of hepatitis C virus (HCV) type 4 in the Seine-Saint-Denis district (France) and indicate seven different HCV-4 subtypes linked to two different epidemiological patterns

Morice

Roulot

Grando

et al. 2001

Journal of General Virology

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 92%

Phylogenetic analyses confirm the high prevalence of hepatitis C virus (HCV) type 4 in the Seine-Saint-Denis district (France) and indicate seven different HCV-4 subtypes linked to two different epidemiological patterns

Morice

Roulot

Grando

et al. 2001

Journal of General Virology

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“…32 33 Serotyping may also be unsuitable for specimens from immunosuppressed patients as they may have insufficient antibody for detection. 32 Concordance between serotyping and genotyping, at least for types 1 to 3, however, was high in our study. It is apparent that for epidemiological purposes hepatitis C types can be established by methods based on serological typing.…”

Section: Discussioncontrasting

confidence: 52%

A national survey of genitourinary medicine clinic attenders provides little evidence of sexual transmission of hepatitis C virus infection

Balogun¹,

Ramsay²,

Parry³

et al. 2003

Sexually Transmitted Infections

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Objective: To determine the prevalence and genetic diversity of hepatitis C virus in genitourinary medicine clinic attenders and to assess the extent of sexual transmission of the virus. Methods: A cross sectional, unlinked, anonymous survey in 14 genitourinary medicine clinics situated in England, Wales, and Northern Ireland. Serum specimens from genitourinary medicine clinic attenders, retained as part of the Unlinked Anonymous Prevalence Monitoring Programme (UAPMP) serum archive, were tested in small pools, for the presence of antibody to hepatitis C virus (anti-HCV). The main outcome measures were prevalence of antibodies to hepatitis C virus and identification of hepatitis C virus genotypes. Results: Testing of 17 586 specimens from 1995 showed an adjusted prevalence of anti-HCV in genitourinary medicine clinic attenders of 1.03% (95% CI: 0.89 to 1.16) overall and 0.65% (95% CI: 0.51 to 0.78) among those who did not report injecting drug use. Prevalence in injecting drug users attending genitourinary medicine clinics was 36.9% in both 1995 and 1996. Heterosexual injecting drug users had a higher prevalence of anti-HCV than homosexual/bisexual injectors. The most common hepatitis C genotypes were types 3a and 1a. There was a high degree of concordance between genotype and serotype. Conclusions: The low prevalence of anti-HCV in genitourinary medicine clinic attenders who deny injecting drugs suggests that the majority of hepatitis C infections have been acquired in adult life, mostly by injecting drug use, and that the hepatitis C virus is rarely transmitted sexually. The use of needle exchanges may explain the relatively low prevalence observed in the injecting drug users.

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“…It is likely that in many of the specimens HCV RNA degraded to undetectable levels. Serotyping has been shown to correlate well with genotyping [33,34] and even though it is unable to differentiate between subtypes, it is able to detect type specific antibodies when HCV RNA is unde tectable. Serotype analysis showed that types 1 and 3 were the most prevalent in each of the three years studied.…”

Section: Discussionmentioning

confidence: 99%