Comparison of group B streptococci colonization in vaginal and rectal specimens by culture method and polymerase chain reaction technique

Bidgani, Shahrokh; Navidifar, Tahereh; Najafian, Mahin; Amin, Mansour

doi:10.1016/j.jcma.2015.06.021

Cited by 27 publications

(22 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since PCR detects only bacterial genes, not viable bacteria colonies, this can enhance the PPV of the test. In our study, the PPV was 86.8%, which was markedly higher than those observed in similar studies conducted by De Paris et al 18 (59%) and Bidgani 28 (52.0-68.0%).…”

Section: Tablecontrasting

confidence: 67%

“…Ideal testing should also detect GBS at birth as accurately and quickly as possible, even when births are premature, as maternal colonization can be transitory, chronic, or intermittent. Both traditional and real-time PCR techniques have been widely investigated in this context 8,18,[26][27][28] . In this study, both the sensitivity and NPV of the molecular method were 100%, respectively.…”

Section: Tablementioning

confidence: 99%

See 1 more Smart Citation

Group B Streptococcus detection in pregnant women via culture and PCR methods

Wollheim

Sperhacke

Fontana

et al. 2017

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Introduction: Group B Streptococcus (GBS), a source of neonatal infection, colonizes the gastrointestinal and genitourinary tracts of pregnant women. Routine screening for maternal GBS in late pregnancy and consequent intrapartum antibiotic prophylaxis have reduced the incidence of early-onset GBS neonatal infection. The aim of this study was to evaluate the performance of PCR, compared to culture (gold standard), in GBS colonization screening of pregnant women, and to establish the prevalence of GBS colonization among this population. Methods: Vaginal introitus and perianal samples were collected from 204 pregnant women, between the 35 th and 37 th weeks of pregnancy, at the Obstetrics and Gynecology Unit of the University of Caxias do Sul General Hospital between June 2008 and September 2009. All samples were cultured after enrichment in a selective medium and then assayed by culture and PCR methods. Results: The culture and PCR methods yielded detection rates of vaginal/perianal GBS colonization of 22.5% and 26%, respectively (sensitivity 100%; specificity 95.6%; positive and negative predictive values 86.8% and 100%, respectively). A higher prevalence of GBS colonization was detected in the combined vaginal and perianal samples by both culture and PCR assay analyses. Conclusions: PCR is a faster and more efficient method for GBS screening, allowing for optimal identification of women who should receive intrapartum antibiotic prophylaxis to prevent newborn infection.

show abstract

Section: Tablecontrasting

confidence: 67%

Section: Tablementioning

confidence: 99%

Group B Streptococcus detection in pregnant women via culture and PCR methods

Wollheim

Sperhacke

Fontana

et al. 2017

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

show abstract

“…Streptococci are spherical and grampositive bacteria, which are the natural flora of the pharynx, skin, and intestines of humans, and if they enter the blood or tissues, cause disease (1). Streptococcus agalactia (S. agalactia), the second group of streptococcal bacteria was recognized as a cause of breast infection in cows since 1970 (2).…”

Section: Introductionmentioning

confidence: 99%

The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR method

Daramroodi

Keshavarzi

Raissi

2018

JBRMS

View full text Add to dashboard Cite

Introduction: Group B Streptococcus (GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. The aim of this study was investigating colony PCR and culture methods to detection of GBS in pregnant women. Materials and methods: Hundred pregnant women, at the 35 th and 37 th weeks of pregnancy, were selected from the Obstetrics and Gynecology Unit of the Moatazedi and Shahid Chamran Hospitals in Kermanshah province. Specimens were collected from vaginal introitus and investigated by selective culture and colony PCR methods. Then, antibiotic resistance tests were performed according to the latest guidelines of Clinical and Laboratory Standards Institute (CLSI). Results: Prevalence of GBS colonization was shown to be 5% and 6% by the culture and colony PCR methods, respectively. Also, resistance rate to erythromycin, penicillin, vancomycin, and the clindamycin were determined to be 50%, 16.66%, 16.66% and 33.33 %, respectively. Moreover, the highest resistance was for erythromycin and the appropriate antibiotics were penicillin and then vancomycin. Conclusion: A higher prevalence of GBS colonization in pregnant women in the Kermanshah city of Iran was detected using colony PCR method compared to culture method.

show abstract

“…In addition, Amin et al 8 found that the positive rate for GBS was 41.6% and 43.8% from rectal and vaginal samples, respectively, using PCR methods. 8 Although the authors did not perform statistical analysis to compare the differences between the culture and PCR methods, the detection rate seemed to be higher when a PCR test was applied. The authors provided an explanation for the low detection rate of the culture method.…”

mentioning

confidence: 99%

“…Unfortunately, the policy of the government in Taiwan only permits us to use one method (culture) and one anatomic site (low vagina) to finish the GBS screening. In order to continue to support the overall strategy of improving global health in Taiwan, such as an annual Papanicolaou smear, bi-annual mammography, 11,12 and GBS screening for pregnant women, 1,8 we encourage more domestic studies to provide better evidence to support the policy of the Taiwanese government.…”

mentioning

confidence: 99%