Group B Streptococcus detection in pregnant women via culture and PCR methods

Wollheim, Cláudia; Sperhacke, Rosa Dea; Fontana, Sabrina Kahler Ribeiro; Vanni, Andréa Cristina; Kato, Sérgio Kakuta; Araujo, Patricia; Barth, Afonso Luís; Madi, José Mauro

doi:10.1590/0037-8682-0454-2016

Cited by 27 publications

(26 citation statements)

References 20 publications

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“…Although the revised CDC guidelines [1] recommend universal culture-based GBS screening between 35 and 37 weeks of pregnancy, the use of molecular methods as an additional rapid method of detection is encouraged. In concordance to our data, increased GBS detection rates by PCR assays compared to culture have been previously reported [15,23] with comparable results [5,6,8,24] or with lower colonization rates than the ones observed in this study [25]. However, previous data showed comparable performance of PCR method to culture [14].…”

Section: Discussionsupporting

confidence: 93%

Antenatal group B streptococcus detection in pregnant women: culture or PCR?

Gerolymatos

Karlovasiti

Sianou

et al. 2018

J Infect Dev Ctries

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Introduction: Group B streptococcus (GBS) is an important cause of neonatal infections. Maternal GBS colonization screening and intrapartum antimicrobial prophylaxis of colonized women can prevent neonatal diseases. The aim of this study was to assess the prevalence of GBS colonization in pregnant and non-pregnant women and to compare the performance of a polymerase chain reaction (PCR) assay with the established as gold standard technique, culture method, used for the detection of this microorganism. Methodology: Vaginal and rectal samples collected from 857 pregnant and 370 non-pregnant women were examined through cultures, while the samples collected from 452 pregnant women between 35 and 37 weeks of gestation were assayed by culture and PCR method targeting the cfb gene. Results: GBS colonization was present in both pregnant and non-pregnant women. The colonization rate was similar in non-pregnant and first trimester pregnant women and then increased from first to the third trimester of pregnancy. GBS cultures for vaginal and rectal samples were positive in 13.2% and 14.3% in non-pregnant women, while in pregnant women 13.2% and 13.7% in the first trimester, and 15.0% and 16.5% in the second trimester, respectively. In third trimester pregnant women, compared to culture method, PCR identified a significantly increased number of GBS positive vaginal (18.4% vs 22.6%, p = 0.0006) and rectal (18.1% vs 21.2%, p = 0.01) samples. Conclusions: GBS colonization rate was higher in the third trimester. PCR proved to be a rapid and useful GBS screening method allowing a shorter detection time, while identifying more colonized women than culture.

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Section: Discussionsupporting

confidence: 93%

Antenatal group B streptococcus detection in pregnant women: culture or PCR?

Gerolymatos

Karlovasiti

Sianou

et al. 2018

J Infect Dev Ctries

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“…Culture-based methods remain the gold standard for GBS detection; however, numerous studies have suggested molecular methods, such as PCR, are more sensitive and specific compared to culture and represent a point-of-care detection option (El Helali et al 2009;de-Paris et al 2011;Khalil et al 2017;Wollheim et al 2017;Relich et al 2018). Cost-effectiveness is another consideration when evaluating screening methods, particularly in GBS where universal screening is implemented (Kaambwa et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Detection of group B Streptococcus during antenatal screening in Western Australia: a comparison of culture and molecular methods

Furfaro

Chang

Payne

2019

J of Applied Microbiology

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Aim Global screening strategies for Group B Streptococcus (GBS) include risk‐ or culture‐based methods to guide intrapartum prophylaxis. In Western Australia (WA), antenatal culture‐based screening is routine; however, numerous culture methods exist, in addition to molecular methods. We aimed to assess the comparability of research and diagnostic screening approaches. Methods and Results Vaginal and rectal swabs were self‐collected by pregnant women (n = 531) from King Edward Memorial Hospital, WA, in parallel to routine screening (35–37 weeks of gestation). Research methods involved culture (Strep B Carrot Broth™ and StrepB CHROMagar™) and molecular methods (real‐time PCR) and were compared to routine diagnostic screening (Lim Broth and Granada agar). Overall, GBS detection was comparable between research and diagnostic approaches (3–5% discrepancy, kappa = 0·76). Specificity/sensitivity of Carrot Broth™ was 100%/89%, while that of CHROMagar™ was 73%/100%, respectively. Direct PCR was unable to detect GBS in ~18% of specimens which were culture positive; however, it exhibited 100% specificity. Conclusions This clinical evaluation of GBS screening methods provides support for current practice. Significance and Impact of the Study Although CHROM was highly sensitive, further testing is recommended due to a high false‐positive rate. Molecular assays are useful for rapid detection; however, low‐titre samples may require additional enrichment prior to molecular analysis to improve sensitivity.

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“…The CDC guidelines also cite other laboratory tests for GBS detection, including PCR methods. PCR-based approaches are gaining a promising role in GBS detection, largely due to their higher sensitivity [6,13,18]. A European consensus statement noted that failure to treat GBS-positive mothers could lead to serious adverse neonatal outcomes.…”

Section: Discussionmentioning

confidence: 99%

Group B Streptococcus detection in pregnant women: comparison of real-time PCR assay, culture, and the Xpert GBS rapid test

Vieira¹,

Perez²,

Machado³

et al. 2019

Preprint

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Background Group B Streptococcus (GBS) is one of the most important causative agents of neonatal sepsis. As administration of prophylactic antibiotics during labor can prevent GBS infection, routine screening for this bacterium in prenatal care before the onset of labor is recommended. However, many women present in labor without having undergone such testing during antenatal care, and the turnaround time of detection methods is insufficient for results to be obtained before delivery. Methods Vaginal and anorectal specimens were collected from 270 pregnant women. Each sample was tested by Xpert GBS, PCR, and culture for GBS detection. Results The overall prevalence of maternal GBS colonization was 30.7% according to Xpert GBS, 51.1% according to PCR, and 14.3% according to cultures. Considering the PCR method as the reference, the Xpert GBS had a sensitivity of 53% and specificity of 93%. Positive Xpert GBS results were associated with marital status (married or cohabitating) and with prematurity as a cause of neonatal hospitalization. Positive cultures were associated with ischemic–hypoxic encephalopathy requiring therapeutic hypothermia. Conclusions Combined enrichment/PCR and the Xpert GBS rapid test found a high prevalence of GBS colonization. The Xpert GBS technique gives faster results and could be useful for evaluating mothers who present without antenatal GBS screening results and are at risk of preterm labor, thus allowing institution of prophylactic antibiotic therapy. Trial Registration: The study was approved by the institutional Research Ethics Committee (CAAE: 59688316.0.0000.5327)

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Group B Streptococcus detection in pregnant women via culture and PCR methods

Cited by 27 publications

References 20 publications

Antenatal group B streptococcus detection in pregnant women: culture or PCR?

Antenatal group B streptococcus detection in pregnant women: culture or PCR?

Detection of group B Streptococcus during antenatal screening in Western Australia: a comparison of culture and molecular methods

Group B Streptococcus detection in pregnant women: comparison of real-time PCR assay, culture, and the Xpert GBS rapid test

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