Comparison of Gold CD-R Types as Electrochemical Device and as Platform for Biosensors

Foguel, Marcos Vinicius; Santos, Glauco Pilon dos; Ferreira, A. A.; Magnani, Marina; Mascini, Marcello; Skládal, Petr; Benedetti, Assis Vicente; Yamanaka, Hideko

doi:10.5935/0103-5053.20150308

Cited by 2 publications

(3 citation statements)

References 2 publications

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“…After the toner is removed, the gold electrodes remain [ 70 ]. In 2016, Foguel et al adapted and characterized this method further, by introducing a polytetrafluoroethylene (PTFE) layer to supplant the toner, facilitating the immobilization of various biological molecules [ 71 ]. These groups have used this approach for electrochemistry applications, but the method, thus far, has not been used to integrate electrodes for electrophoretic separations.…”

Section: Integrated Electrodesmentioning

confidence: 99%

Electrode Materials in Microfluidic Systems for the Processing and Separation of DNA: A Mini Review

Birch

Landers

2017

Micromachines

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Since the advent of genetic analysis, electrode materials have played an irreplaceable role due to the easily-exploitable negatively-charged backbone of the DNA structure. Initially, the employment of electrophoretic movement lay only in the separation of DNA fragments of differing length; however, the widening utility of electrokinetic phenomena at the microscale in areas such as fluid transportation and multistep integration led researchers to capitalize further when translating processes to microfluidic or “lab-on-chip” devices. Over the following three decades, the field witnessed a plethora of ways in which the necessary voltages could be transmitted to the sample and reagents with many successes; however, additional demands were then placed on those hoping to bring their microdevices to the market place. A greater emphasis on the cost of all constituent parts along with the increased importance that fluidics be contained hermetically at all times meant groups would become more imaginative when incorporating electrode materials. This review will aim to exactly describe the evolution of how those materials have been employed in DNA-based microfluidic devices. It will focus on how developers began to explore other emerging uses and also discuss how their tactics reflected the progressive demands of their chosen industry.

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Section: Integrated Electrodesmentioning

confidence: 99%

Electrode Materials in Microfluidic Systems for the Processing and Separation of DNA: A Mini Review

Birch

Landers

2017

Micromachines

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“…This work reports the development of an impedimetric immunosensor for the determination of aflatoxin B 1 , through gold CD-trode (electrode obtained from recordable compact disks) surface modification with a self-assembled monolayer (SAM) of lipoic acid activated via EDC (1-ethyl-3-(3-(dimethylamino)-propyl)carbodiimide)/NHS (N-hydroxy succinimide) for the immobilization of anti-aflatoxin B 1 antibody. CD-trodes can be easily obtained by simple treatment of wasted gold CDs to obtain cheap but efficient electrochemical sensors [26,27]. The optimization of the experimental parameters involved in the development of the CD-trode sensor was performed chemometrically, by means of full factorial design.…”

Section: Matrixmentioning

confidence: 99%

“…The electrical contact of the CD-trode was a laminated copper wire fixed and insulated with polytetrafluoroethylene (PTFE) tape. The characterization of the AuCD-trode as an electrochemical transducer was done previously [27]. CD-trodes are disposable, so they were used only once.…”

Section: Construction Of Gold Electrode (Aucd-trode)mentioning

confidence: 99%

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

et al. 2016

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This work describes the investigation of a label-free immunosensor for the detection of aflatoxin B 1 (AFB 1 ). CD-trodes (electrodes obtained from recordable compact disks) were used as low-cost and disposable transducers after modification with a self-assembled monolayer (SAM) of lipoic acid. The anti-aflatoxin B 1 antibody was immobilized via EDC/NHS activation, followed by blocking with bovine serum albumin and immunoassays with AFB 1 . The optimization of analytical parameters and the detection were carried out using electrochemical impedance measurements. Using chemometric tools, the best conditions for the immunosensor development were defined as: anti-AFB 1 antibody at 1:2000 dilution and surface blocking with 0.5% bovine serum albumin, both incubated for 1 h, and antibody-antigen immunoreaction for 30 min. The impedimetric immunosensor showed a linear range from 5 × 10 −9 to 1 × 10 −7 mol·L −1 (1.56-31.2 ng·mL −1 ), limit of detection and limit of quantification, respectively, 3.6 × 10 −10 and 1.1 × 10 −9 mol·L −1 (0.11 and 0.34 ng·mL −1 ). The proposed immunosensor was applied to analyze peanut samples.

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Comparison of Gold CD-R Types as Electrochemical Device and as Platform for Biosensors

Cited by 2 publications

References 2 publications

Electrode Materials in Microfluidic Systems for the Processing and Separation of DNA: A Mini Review

Electrode Materials in Microfluidic Systems for the Processing and Separation of DNA: A Mini Review

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

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