Comparison of Gliadin and Glutenin Subunits in the Triticinae by SDS-Polyacrylamide Gel Electrophoresis

Polymorphism in seed endosperm proteins (gliadins and glutenins) of Turkish cultivated einkorn wheat [Triticum monococcumssp.monococcum] landraces

“…When we compared the results obtained from gliadin electrophoresis in the present study with previous studies; we obtained 82 different gliadin patterns, which were in agreement with 80 (in 109 T. monoccum L. accessions) by Metakovsky and Baboev (1992) and 224 (72 for T. urartu in 150 accessions, 100 for T. boeticum in 160 accessions, and 52 for T. monoccum in 74 accessions) by Ciaffi et al (1997) and are higher than the gliadin patterns obtained by others; 10 (five for each of T. monococcum and A. squarrosa) by Preston et al (1975), 13 (four different allelic variants for T. boeoticum and nine for T. urartu) by Ciaffi et al (1992), 56 (in 490 T. tauschii accessions) by William et al (1993). These differences might be existed due to different species, and the number of samples used as representatives of accessions or populations in other studies.…”

“…For HMW-glutenin, we observed seven glutenin subunits, which is less than previous studies; 14 Glu D t 1 subunit (490 T. tauschii accessions) by William et al (1993), 40 subunits for einkorn wheat (26 for T. monococcum and 14 T. boeoticum) and 12 alleles for Glu-A1 locus (seven in T. urartu and five in T. boeoticum) by Ciaffi et al (1992) and higher than five HMW-glutenin subunits for each T. monococcum and A. squarrosa by Preston et al (1975). However we observed a genetic diversity high according to HMW-glutenin subunits in contrast to previous studies (Waines and Payne 1987;Rodríguez-Quijano et al 1997).…”

“…The results show that the genetic diversity found in gliadin subunits (H t = 0.17) is lower compared to previous studies (Preston et al 1975;Waines and Payne 1987;Ciaffi et al 1992Ciaffi et al , 1997Metakovsky and Baboev 1992;Sofalian and Varizadeh 2009;Hu et al 2011). However, the genetic diversity of gliadins are still higher than the results obtained from isoenzymes studies (Smith-Huerta et al 1989;Peókircioçlu et al 1998;Hedge et al 2000;Karcicio et al 2003), but less polymorphic than Mendlinger and Zohary (1995).…”

“…Preston et al (1975) compared gliadin and glutenin compositions of wheat (Triticum aestium), einkorn (Triticum monococcum), A. squarrosa, and S. cereale using the SDS-PAGE method. Metakovsky and Baboev (1992) reported the polymorphism and inheritance of gliadin polypeptides in Triticum monococcum L. Ciaffi et al (1992) represented through the evaluation of a large collection of wild wheat progenitors consisting of diploid (Triticum boeticum Boiss and Triticum urartu Thum.)…”

Effect of low-dose irradiation on soybean protein solubility, trypsin inhibitor activity, and protein patterns separated by polyacrylamide gel electrophoresis

“…The irradiated and the control materials were milled in an electric mill (Janke and Kunkel Micro-Fein Muhle Culalli) to pass through a 1.5-mm sieve. The seed meal was defatted with a mixture of chloroform-benzene (Preston et al, 1975). The results were calculated as the average of three samples in each case.…”