Methods for Characterization of the Seed Proteins in Cereals and Legumes

Stegemann, Hermann; Pietsch, G.

doi:10.1007/978-94-009-6801-1_3

Cited by 11 publications

(4 citation statements)

References 107 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Total protein quantification was conducted using the official method described by [59], which is based on the quantification of total nitrogen content in the sample as described by Kjedahl (Method 981.10) using the conversion factor of total nitrogen to protein content determined by [60] in quinoa, equivalent to a value of 5.39. Extraction of albumin and globulin protein fractions was conducted according to the protocols described by [61,62], with some modifications [17].…”

Section: Field Establishment and Experimental Designmentioning

confidence: 99%

Production Efficiency and Total Protein Yield in Quinoa Grown under Water Stress

et al. 2021

View full text Add to dashboard Cite

The increasing water scarcity affects the agricultural sector, and it is a significant constraining factor for crop production in many areas of the world. Water resource management and use related to crop productivity is the most important factor in many crops. Since consumer demands healthy food, the nutritive quality and the active ingredient need to be considered within the productive issue. The objective of this study was to determine water technical efficiency related to seed yield and seed protein content and composition in quinoa (Chenopodium quinoa Willd.) under water stress using data envelopment analysis (DEA). The study was conducted in Chillan, Chile in two growing seasons. As water availability increased, seed yield, globulin, and albumin yield increased, particularly in the genotype Cahuil. The higher average efficiency levels for the DEA were 46.7% and 39.2% in Cahuil in both seasons at 20% available water (AW). The highest average efficiency of globulin yield was recorded in the same genotype (Cahuil). The highest multi-product technical efficiency levels in all input and output included in this study were observed in Cahuil, Regalona, and Morado under water scarcity in both seasons. In future studies related to crop management, DEA provides a good framework for estimating efficiency under restricted factors and multi-product results.

show abstract

Section: Field Establishment and Experimental Designmentioning

confidence: 99%

Production Efficiency and Total Protein Yield in Quinoa Grown under Water Stress

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Defatting of the flour samples was considered appropriate because lipids are known to interfere with hydrophylic extraction procedures (Stegemann & Pietsch, 1983). However, defatting the starting material can also affect the subsequent extraction of the proteins (Byers et ai., 1983).…”

Section: Analysis Of Floursmentioning

confidence: 99%

“…Many studies have quantified protein extraction by either single extractions or the sequential use of different solvents (Byers, Miflin & Smith, 1983). However, a large number of variables such as sample size, de-fatting (delipidating), different sequential solvents, the number of extractions and their timing, sample to solvent ratio, temperature and intensity of extraction were reported to have an effect on protein extraction (Stegemann & Pietsch, 1983;Lasztity, 1984), complicating the choice of an appropriate fractionation technique.…”

Section: Introductionmentioning

confidence: 99%

Comparison of protein extraction procedures to assess the effect of quantitative protein composition on the bread-making quality of South African winter wheat cultivars

Lill

Wentzel

Smith³

et al. 1993

South African Journal of Plant and Soil

View full text Add to dashboard Cite

A suitable technique for the extraction of wheat flour protein fractions was pursued to assess the effect of quantitative protein composition on the bread-making quality of winter wheat cultivars on a large scale. Nine procedures involving the use of different sequential solvent extractions were quantitatively compared. The distribution of protein components in the different solubility classes was checked by means of sodium dodecyl sulphate polyacrylamide gel electrophoresis. T,he most effective procedure to fractionate the four major protein groups sequentially was: albumin (water): globulin (0.5 M NaCI + 0.05 mM Na-EDTA), gliadin [55%To validate this procedure, linear correlations between quality parameters and protein extracted for all localities and cultivars were complemented by principal component and best subset regression analysis. Albumin and globulin were weakly correlated with quality attributes whereas gliadin and glutenin were highly positively correlated. The contribution of relative glutenin content to dough strength was also confirmed. Since these results complied with present concepts on the relationships between protein fractions and functional properties, it was concluded that the technique could be applied for the protein fractionation of South African winter wheat cultivars. am die effek van kwantitatiewe prote·iensamestelling op broodbakkwaliteit op groot skaal te ondersoek, is die wenslikheid van verskeie prote·ienekstraksieprosedures nagegaan. Nege prosedures, wat uit verskillende ekstraheermiddelkombinasies bestaan het, is kwantitatief met mekaar vergelyk. Die verspreiding van prote·ienkomponente binne oplosbaarheidsklasse is met behulp van natrium dodesiel sulfaat poliakrielamiedgelelektroforese nagegaan. Die effektiefste prosedure om die vier prominente prote"ien fraksies opeenvolgend te skei, was: albumien (water), globulien (0.5 M NaCI + 0.05 mM Na-EDTA), gliadien [55% (v/v) propan-2-01] en glutenien [0.0125 M Na2B4~' 0.043 M NaOH, 0.5% (m/v) SDS, 1 % (v/v) ~-mercaptoethanol] .Om die toepassing van die tegniek te toets, is die verband tussen kwantitatiewe prote·iensamestelling en kwaliteitseienskappe met liniere korrelasies, hoofkomponent-en beste substel regressie analise oor lokaliteite en cultivars bereken. Albumien en globulien is swak met kwaliteitseienskappe gekorreleer, terwyl gliadien en glutenien hoogs beduidend gekorreleer is. Die bydrae van glutenien-inhoud tot deegsterkte is ook bevestig. Hierdie resultate kom ooreen met bevindinge rakende die verband tussen prote·ienfraksies en funksionele eienskappe. Die tegniek is dus vir die fraksionering van Suid Afrikaanse winterkoringprote"ien geskik.

show abstract

“…Protein fractionation by SDS-PAGE (Stegemann and Pietsch 1983) is relatively rapid and inexpensive compared to isozyme and some DNA analyses, especially when conducted with precast minigels. In contrast, two-dimensional electrophoresis will often reveal an astounding number of different seed proteins simultaneously, but it is relatively slow and demands considerable technical skill and experience.…”

Section: Proteinsmentioning

confidence: 99%

Genetic Markers and Plant Genetic Resource Management

Bretting,

Widrlechner

1993

HortSci

View full text Add to dashboard Cite

When suitably deployed, genetic markers facilitate all phases of plant genetic resource management, from acquisition through enhancement. This paper will review the kinds of plant genetic resources and genetic markers, analytical methods for marker data, and specific applications of genetic markers to plant genetic resource management, such as (i) assessing a collection's “gaps” and redundancy; sampling strategies; characterizing newly acquired germplasm; maintaining “trueness to type”; monitoring genetic shifts; monitoring germplasm viability and health; developing optimal utilization strategies from genetic marker data; exploiting associations among horticulturally meritorious traits and genetic markers. Finally, general conclusions and forecasts regarding future prospects for applying genetic markers to these tasks will be presented.

show abstract

Methods for Characterization of the Seed Proteins in Cereals and Legumes

Cited by 11 publications

References 107 publications

Production Efficiency and Total Protein Yield in Quinoa Grown under Water Stress

Production Efficiency and Total Protein Yield in Quinoa Grown under Water Stress

Comparison of protein extraction procedures to assess the effect of quantitative protein composition on the bread-making quality of South African winter wheat cultivars

Genetic Markers and Plant Genetic Resource Management

Contact Info

Product

Resources

About