Comparison of Genotypes and Antibiotic Resistances of Campylobacter jejuni and Campylobacter coli on Chicken Retail Meat and at Slaughter

Kittl, Sonja; Korczak, Bożena; Niederer, Lilian; Baumgärtner, A.; Buettner, S.; Overesch, Gudrun; Kuhnert, Peter

doi:10.1128/aem.00493-13

Cited by 21 publications

(24 citation statements)

References 14 publications

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“…In this study, analysis of the genetic similarities between isolates revealed similar results to those previously reported, i.e. suggesting that C. coli isolates were less diverse, based on the AFLP profiles, that C. jejuni isolates [ 39 ], probably due to C. coli showing more host specificity than C. jejuni [ 40 ].…”

Section: Discussionsupporting

confidence: 87%

Virulence characteristics of hcp + Campylobacter jejuni and Campylobacter coli isolates from retail chicken

et al. 2015

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BackgroundRecently the Type VI secretion system (T6SS), which can play a significant role in bacterial survival and pathogenesis, was reported in Campylobacter spp., having the hcp gene as a key component.MethodsCampylobacteriosis is associated with the consumption of infected chicken meat. Our study aimed to explore the presence of T6SS in C. jejuni (n = 59) and C. coli (n = 57) isolates, from retail raw chicken and to investigate their pathogenic potential. The hcp gene was used as an indicator for the T6SS presence.ResultsUsing multiplex PCR we have identified a significantly higher prevalence of hcp in C. coli isolates (56.1%) than in C. jejuni (28.8%) and AFLP analysis of the isolates showed a high degree of genetic similarity between the isolates carrying the hcp gene. Genome sequencing data showed that 84.3% of the C. coli and 93.7% of the C. jejuni isolates had all 13 T6SS open reading frames. Moreover, the virulence characteristics of hcp + isolates, including motility and the ability to invade human intestinal epithelial cells in vitro, were significantly greater than in the control strain C. jejuni 12502; a human isolate which is hcp positive.ConclusionOverall, it was discovered that hcp+C. coli and C. jejuni isolated from retail chicken isolates posses genetic and phenotypic properties associated with enhanced virulence. However, since human infections with C. coli are significantly less frequent than those of C. jejuni, the relationship between virulence factors and pathogenesis requires further study.

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Section: Discussionsupporting

confidence: 87%

Virulence characteristics of hcp + Campylobacter jejuni and Campylobacter coli isolates from retail chicken

et al. 2015

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“…Our analyses indicated 31% of cattle strains being resistant to quinolones, and only 1% resistant to macrolides (represented by only one C. coli strain). Similar rates of resistance were found in Switzerland for Campylobacter isolated from other animal species like chicken, pig and dog [29][30][31]37]. Antibiotic resistance is more pronounced in human isolates whereas macrolide resistance is virtually absent [5].…”

Section: Discussionsupporting

confidence: 71%

Genotypes and antibiotic resistance of bovineCampylobacterand their contribution to human campylobacteriosis

et al. 2014

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Campylobacter jejuni and Campylobacter coli are the most important bacterial causes of human gastroenteritis. Chicken has been recognized as a major source for human infection, whereas cattle might also contribute to a lesser extent. However, there is a paucity of information available regarding Campylobacter in Swiss cattle and their role for human campylobacteriosis. To gain more information on genotypes and antibiotic resistance of bovine C. jejuni and C. coli and on their contribution to human disease, 97 cattle isolates were analysed. Multilocus sequence typing (MLST) and flaB typing were applied and the gyrA and 23S rRNA genes were screened for point mutations responsible for quinolone and macrolide resistance, respectively. A total of 37 sequence types (STs) and 44 flaB types were identified, including two sequence types and five flaB types not previously described. Most common sequence types were ST21 (21%), ST61 (12%) and ST48 (11%). Only one isolate was macrolide resistant while 31% (n = 30) were quinolone resistant. Source attribution indicated chicken as the main source of human infection with cattle being second. In conclusion, cattle should not be underestimated as a potential source of human campylobacteriosis.

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“…The results of a previous study by Padungtod et al (2006) on the level of resistance in isolates from chicken cecal contents and chicken meat also showed similar resistance between these two sectors. Another study, conducted in Switzerland by Kittl et al (2013), demonstrated considerable overlap in the AMR level of Campylobacter isolates collected from chickens in abattoirs in comparison with isolates obtained from chicken meat at retail. In addition, Idris et al (2006) found that fluoroquinolone resistance in Campylobacter can spread through an integrated commercial poultry production system from parental flocks to their progeny as both groups were colonized by the same strain of resistant C. coli.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance of Campylobacter in Broiler Chicken Along the Food Chain in Canada

Dramé

Leclair

Parmley

et al. 2020

Foodborne Pathogens and Disease

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Antimicrobial resistance (AMR) is a major public health threat worldwide. The main objective of this study was to compare AMR in Campylobacter from broiler chickens raised on Canadian farms and their products in different geographical regions of Canada. To do this, antimicrobial susceptibility results from isolates of Campylobacter recovered from a national microbiological baseline study conducted in federally registered establishments and in the retail marketplace were analyzed. Among 1460 isolates tested, 774 (53%) were resistant to at least one antimicrobial, with a predominance of three profiles: tetracycline (39%), quinolonetetracycline (6.6%), and quinolones only (3.5%). The results showed no significant difference in the frequency of resistant profiles (p ‡ 0.05) among the isolates originating from different points in the food processing chain at slaughterhouses and in retail establishments. This suggests that AMR observed in Campylobacter isolates from raw chicken at retail originated further upstream in the system. A difference in the frequency of certain resistance profiles was observed between the regions of Canada. For instance, in British Columbia, there was more resistance to quinolones, while in Ontario and Quebec, Campylobacter isolates were more resistant to tetracyclines, macrolides, ketolides, and lincosamides. Comparison of AMR data from this study with those from the Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS) did not show any significant difference and provides evidence that CIPARS produces nationally representative resistance results.

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Comparison of Genotypes and Antibiotic Resistances of Campylobacter jejuni and Campylobacter coli on Chicken Retail Meat and at Slaughter

Cited by 21 publications

References 14 publications

Virulence characteristics of hcp + Campylobacter jejuni and Campylobacter coli isolates from retail chicken

Virulence characteristics of hcp + Campylobacter jejuni and Campylobacter coli isolates from retail chicken

Genotypes and antibiotic resistance of bovineCampylobacterand their contribution to human campylobacteriosis

Antimicrobial Resistance of Campylobacter in Broiler Chicken Along the Food Chain in Canada

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