Comparison of Gene Expression in Peri-implant Soft Tissue and Oral Mucosal Tissue by Microarray Analysis

Rat peri‐implant soft tissue specifically expressedCXCL2on titanium implant during wound healing

“…23 Taken together, these results suggest that these standard inflammatory markers are not specific markers in PIST. In contrast, all of the PIST-specific inflammatory markers tested here, Ccl3, CEACAM1, Cfi, CXCL2, Csf3, Ifitm1, IL-1β, Sectm1b, and Serping1 which were selected from our previous PIST-OMT comparison database, 16 were expressed at higher levels in PIST than in both control tissues.…”

“…16,24 Ifitm1 is known to modulate immune responses through interferons and is expressed in peri-implant epithelium. 16,25 IL-1β is generally produced from monocytes or macrophages in the early stage of wound healing to activate immune cells and is reported to be expressed in healthy peri-implant tissue. 26,27 IL-1β is also reported to be upregulated in healthy PIST in comparison to the gingiva.…”

“…21 In the systematic review about expression level of standard inflammatory makers in PICF, it was concluded that there is no characteristic expression of inflammatory markers during wound healing. 22 However, the expression of PIST-specific inflammatory markers, defined in our previous study, 16 has not yet been investigated.…”

“…The Gene Expression Omnibus database (accession number: GSE43744), which was previously used to compare PIST and OMT tissues, 16 was used for selecting PIST‐specific inflammatory markers. Nine genes were selected by the fold change value of their microarray signals in PIST (>10‐fold change) and by using Gene Ontology analysis (i.e., genes involved in the inflammatory response): chemokine Ccl3 , CEACAM1 , Cfi , CXCL2 , Csf3 , Ifitm1 , IL‐1β , Sectm1b , and Serping1 .…”

“…We previously used microarray technology to investigate specific gene expression in PIST in order to identify the key factors in PIST's defensive mechanism 16 . This study identified over 700 genes that were expressed in PIST at significantly higher levels than in oral mucosal tissue (OMT).…”