Comparison of gamma-decalactone biosynthesis by yeast Yarrowia lipolytica MTLY40-2p and W29 in batch-cultures

Abstract

Gamma-decalactone is an interesting flavuring compound with an intense oily-peachy aroma. It is commonly used in food and cosmetic industry. Biotechnological methods of its synthesis are based on the processes of fatty acid degradation, mainly ricinoleic acid, which is the main component of castor oil. The aim of this study was to compare the productivity of gamma-decalactone in batch cultures of two strains of Yarrowia lipolytica, wild strain W29 and its derived mutant MTLY40-2p, grown in flasks and a bioreactor. We analyzed the concentration of gammadecalactone in the aqueous and oil phase, the optical density of the medium, the yield of yeast dry matter and the particle size distribution in the particular media. The modified strain had a higher aroma synthesis capacity. This strain grown in flasks produced thrice as much gammadecalactone (5.5 ± 0.16 g/L) as compared to the wild-type strain (1.8 ± 0.03 g/L). During the 7-day biotransformation, the mutant strain did not have the ability to degrade gamma-decalactone, whereas in the biotransformation using the wild-type strain, reutilization was observed after the third day. Regardless of the type of culture, larger amounts of gamma-decalactone were accumulated in the lipid phase. Cultivation in a bioreactor resulted in a lower biomass yield and a lower concentration of lactone compared to flask culture, regardless of the strain used. The MTLY40-2p strain grew in mycelium form and tended to aggregate cells.

Characteristics of bioactive components in fermented Sargassum and Ulva Seaweed using SCOBY as potential anti-diabetic candidates

“…The detected fermentation by-products included 2(5H)-furanone as a flavor component, γ-decalactone as an aromatic compound, and benzaldehyde as a volatile flavor compound. γ-Decalactone was synthesized by yeast Yarrowia lipolytica through oxidative degradation of fatty acids [29]. A similar metabolic process yields γdecalactone in kombucha through the activity of the bacterium Acetobacter indonesiensis [30].…”

Application of Yarrowia lipolytica in fermented beverages

“…γdecalactone is the most interesting of these and the one with the most focus in the literature. It is described as pleasantly oily-peachlike (Braga and Belo, 2016;Małajowicz et al, 2020). It has been found that the main component of castor oil, ricinoleic acid, a C18hydroxylated fatty acid, is one of the most promising substrates for optimised production of γ-decalactone (Braga and Belo, 2013;Braga and Belo, 2015;Braga and Belo, 2016;Małajowicz et al, 2020;Kothari et al, 2022).…”

“…It is described as pleasantly oily-peachlike (Braga and Belo, 2016;Małajowicz et al, 2020). It has been found that the main component of castor oil, ricinoleic acid, a C18hydroxylated fatty acid, is one of the most promising substrates for optimised production of γ-decalactone (Braga and Belo, 2013;Braga and Belo, 2015;Braga and Belo, 2016;Małajowicz et al, 2020;Kothari et al, 2022). More than 400 fatty acids are found in milk, with the diversity originating from which animal it is derived from and what feed is given (Barłowska and Litwińczuk, 2009;Markiewicz-Kęszycka et al, 2013;Djordjevic et al, 2019).…”

The mechanism of the interaction between the formed emulsification and yeast cells in biotransformation system

“…Gamma-decalactone (GDL), a kind of safe natural aroma compound with an oily-peachy-like flavor, 1,2 is in great demand in the field of foods, pharmaceuticals, and cosmetics. Traditionally, natural GDL mainly relies on plant extraction which is severely restricted by region and season, and its market price is extremely high (US$ 6000 kg −1 , about 40 times that of chemical synthetic products) with a problem of insufficient market supply.…”

“…Usually substrates used for biotransformation are ricinoleic acid (RA), castor oil and ricinic acid methyl ester (RAME), which is based on the biotransformation mechanism of degradation of fatty acid. 2 As a result, the substrate, used in the process of biotransformation to prepare GDL, is a fat-soluble substance. The biotransformation is carried out in oil-water phase system which automatically generate emulsifier and form a homogeneous emulsion system ultimately.…”